Effects of artificially introduced Enterococcus faecalis strains in experimental necrotizing enterocolitis

Patrick T. Delaplain, Brandon A. Bell, Jin Wang, Mubina Isani, Emily Zhang, Christopher P. Gayer, Anatoly V. Grishin, Henri R. Ford

Research output: Contribution to journalArticle

Abstract

Enterococcus faecalis is a ubiquitous intestinal symbiont and common early colonizer of the neonatal gut. Although colonization with E. faecalis has been previously associated with decreased pathology of necrotizing enterocolitis (NEC), these bacteria have been also implicated as opportunistic pathogens. Here we characterized 21 strains of E. faecalis, naturally occurring in 4-day-old rats, for potentially pathogenic properties and ability to colonize the neonatal gut. The strains differed in hemolysis, gelatin liquefaction, antibiotic resistance, biofilm formation, and ability to activate the pro-inflammatory transcription factor NF-κB in cultured enterocytes. Only 3 strains, BB70, 224, and BB24 appreciably colonized the neonatal intestine on day 4 after artificial introduction with the first feeding. The best colonizer, strain BB70, effectively displaced E. faecalis of maternal origin. Whereas BB70 and BB24 significantly increased NEC pathology, strain 224 significantly protected from NEC. Our results show that different strains of E. faecalis may be pathogenic or protective in experimental NEC.

Original languageEnglish (US)
Article numbere0216762
JournalPloS one
Volume14
Issue number11
DOIs
StatePublished - Jan 1 2019

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enterocolitis
Necrotizing Enterocolitis
Enterococcus faecalis
Pathology
Biofilms
Pathogens
Liquefaction
Gelatin
Rats
Bacteria
Transcription Factors
digestive system
Enterocytes
Anti-Bacterial Agents
Microbial Drug Resistance
Hemolysis
enterocytes
hemolysis
Intestines
gelatin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Effects of artificially introduced Enterococcus faecalis strains in experimental necrotizing enterocolitis. / Delaplain, Patrick T.; Bell, Brandon A.; Wang, Jin; Isani, Mubina; Zhang, Emily; Gayer, Christopher P.; Grishin, Anatoly V.; Ford, Henri R.

In: PloS one, Vol. 14, No. 11, e0216762, 01.01.2019.

Research output: Contribution to journalArticle

Delaplain, PT, Bell, BA, Wang, J, Isani, M, Zhang, E, Gayer, CP, Grishin, AV & Ford, HR 2019, 'Effects of artificially introduced Enterococcus faecalis strains in experimental necrotizing enterocolitis', PloS one, vol. 14, no. 11, e0216762. https://doi.org/10.1371/journal.pone.0216762
Delaplain, Patrick T. ; Bell, Brandon A. ; Wang, Jin ; Isani, Mubina ; Zhang, Emily ; Gayer, Christopher P. ; Grishin, Anatoly V. ; Ford, Henri R. / Effects of artificially introduced Enterococcus faecalis strains in experimental necrotizing enterocolitis. In: PloS one. 2019 ; Vol. 14, No. 11.
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