Effects of albuterol enantiomers on ciliary beat frequency in ovine tracheal epithelial cells

Jeffrey I. Frohock, Corrine Wijkstrom-Frei, Matthias A Salathe

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Adrenergic agonists stimulate ciliary beat frequency (CBF), an integral part of mucociliary clearance. To evaluate the differential effects of albuterol enantiomers and their racemic mixture on ciliary function, CBF and intracellular calcium were measured at room temperature from single ovine airway epithelial cells with use of digital videomicroscopy. Baseline CBF was 7.2 ± 0.2 (SE) Hz (n = 80 measurements). R-albuterol (10 μM to 1 mM) stimulated CBF in a dose-dependent manner to maximally 24.4 ± 5.4% above baseline. Racemic albuterol stimulated CBF to maximally 12.8 ± 3.6% above baseline, a significantly lower increase compared with R-albuterol alone, despite identical R-enantiomer amounts in both groups. Simultaneous recordings of intracellular calcium concentration and CBF from single cells indicated that the CBF increase in response to R-albuterol was mediated through β-receptors and stimulation of protein kinase A, in a calcium-dependent and -independent fashion. S-albuterol had a negligible effect on CBF and did not change intracellular calcium. Together, these results suggest that R-albuterol is more efficacious than racemic albuterol in stimulating CBF. Thus S-albuterol may interfere with the ability of R-albuterol to increase CBF.

Original languageEnglish
Pages (from-to)2396-2402
Number of pages7
JournalJournal of Applied Physiology
Volume92
Issue number6
StatePublished - Jul 2 2002

Fingerprint

Albuterol
Sheep
Epithelial Cells
Calcium
Mucociliary Clearance
Video Microscopy
Adrenergic Agonists
Cyclic AMP-Dependent Protein Kinases
Temperature

Keywords

  • Airway
  • Beta-agonists
  • Calcium
  • Signaling

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Frohock, J. I., Wijkstrom-Frei, C., & Salathe, M. A. (2002). Effects of albuterol enantiomers on ciliary beat frequency in ovine tracheal epithelial cells. Journal of Applied Physiology, 92(6), 2396-2402.

Effects of albuterol enantiomers on ciliary beat frequency in ovine tracheal epithelial cells. / Frohock, Jeffrey I.; Wijkstrom-Frei, Corrine; Salathe, Matthias A.

In: Journal of Applied Physiology, Vol. 92, No. 6, 02.07.2002, p. 2396-2402.

Research output: Contribution to journalArticle

Frohock, JI, Wijkstrom-Frei, C & Salathe, MA 2002, 'Effects of albuterol enantiomers on ciliary beat frequency in ovine tracheal epithelial cells', Journal of Applied Physiology, vol. 92, no. 6, pp. 2396-2402.
Frohock, Jeffrey I. ; Wijkstrom-Frei, Corrine ; Salathe, Matthias A. / Effects of albuterol enantiomers on ciliary beat frequency in ovine tracheal epithelial cells. In: Journal of Applied Physiology. 2002 ; Vol. 92, No. 6. pp. 2396-2402.
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