Effects of aging on proliferation and E47 transcription factor activity induced by different stimuli in murine splenic B cells

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Abstract

In the present paper, we have investigated the effects of aging on the expression and function of the E2A-encoded transcription factor E47 in splenic B lymphocytes, unactivated or activated with different stimuli (LPS, anti-CD40, anti-IgM, alone or in combination with IL-4). Results indicate that unstimulated splenic B cells show very low E47 protein levels as well as E47 DNA-binding activity and that, upon B cell activation, E47 expression and DNA-binding activity are strongly induced in young and, to a significantly lesser extent, in old mice. The level of E47 protein expression in stimulated splenic B cells was found significantly higher in young than in old mice, suggesting that DNA-binding activity correlates with protein expression. These results altogether suggest that the reduced expression of the transcriptional regulator E47 could help explain the reduced B cell functions in aging mice.

Original languageEnglish
Pages (from-to)361-369
Number of pages9
JournalMechanisms of Ageing and Development
Volume124
Issue number4
DOIs
StatePublished - Apr 1 2003

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B-Lymphocytes
Transcription Factors
Aging of materials
Cells
DNA
Proteins
Lymphocytes
Interleukin-4
Chemical activation

Keywords

  • Aging
  • B lymphocytes
  • E47 transcription factor
  • Mouse
  • Spleen

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

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abstract = "In the present paper, we have investigated the effects of aging on the expression and function of the E2A-encoded transcription factor E47 in splenic B lymphocytes, unactivated or activated with different stimuli (LPS, anti-CD40, anti-IgM, alone or in combination with IL-4). Results indicate that unstimulated splenic B cells show very low E47 protein levels as well as E47 DNA-binding activity and that, upon B cell activation, E47 expression and DNA-binding activity are strongly induced in young and, to a significantly lesser extent, in old mice. The level of E47 protein expression in stimulated splenic B cells was found significantly higher in young than in old mice, suggesting that DNA-binding activity correlates with protein expression. These results altogether suggest that the reduced expression of the transcriptional regulator E47 could help explain the reduced B cell functions in aging mice.",
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AU - Nguyen, Diep

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AU - Blomberg, Bonnie B

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