Abstract
In the present paper, we have investigated the effects of aging on the expression and function of the E2A-encoded transcription factor E47 in splenic B lymphocytes, unactivated or activated with different stimuli (LPS, anti-CD40, anti-IgM, alone or in combination with IL-4). Results indicate that unstimulated splenic B cells show very low E47 protein levels as well as E47 DNA-binding activity and that, upon B cell activation, E47 expression and DNA-binding activity are strongly induced in young and, to a significantly lesser extent, in old mice. The level of E47 protein expression in stimulated splenic B cells was found significantly higher in young than in old mice, suggesting that DNA-binding activity correlates with protein expression. These results altogether suggest that the reduced expression of the transcriptional regulator E47 could help explain the reduced B cell functions in aging mice.
| Original language | English |
|---|---|
| Pages (from-to) | 361-369 |
| Number of pages | 9 |
| Journal | Mechanisms of Ageing and Development |
| Volume | 124 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1 2003 |
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Keywords
- Aging
- B lymphocytes
- E47 transcription factor
- Mouse
- Spleen
ASJC Scopus subject areas
- Aging
- Biochemistry
- Developmental Biology
- Developmental Neuroscience
Cite this
Effects of aging on proliferation and E47 transcription factor activity induced by different stimuli in murine splenic B cells. / Frasca, Daniela; Nguyen, Diep; Riley, Richard L; Blomberg, Bonnie B.
In: Mechanisms of Ageing and Development, Vol. 124, No. 4, 01.04.2003, p. 361-369.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Effects of aging on proliferation and E47 transcription factor activity induced by different stimuli in murine splenic B cells
AU - Frasca, Daniela
AU - Nguyen, Diep
AU - Riley, Richard L
AU - Blomberg, Bonnie B
PY - 2003/4/1
Y1 - 2003/4/1
N2 - In the present paper, we have investigated the effects of aging on the expression and function of the E2A-encoded transcription factor E47 in splenic B lymphocytes, unactivated or activated with different stimuli (LPS, anti-CD40, anti-IgM, alone or in combination with IL-4). Results indicate that unstimulated splenic B cells show very low E47 protein levels as well as E47 DNA-binding activity and that, upon B cell activation, E47 expression and DNA-binding activity are strongly induced in young and, to a significantly lesser extent, in old mice. The level of E47 protein expression in stimulated splenic B cells was found significantly higher in young than in old mice, suggesting that DNA-binding activity correlates with protein expression. These results altogether suggest that the reduced expression of the transcriptional regulator E47 could help explain the reduced B cell functions in aging mice.
AB - In the present paper, we have investigated the effects of aging on the expression and function of the E2A-encoded transcription factor E47 in splenic B lymphocytes, unactivated or activated with different stimuli (LPS, anti-CD40, anti-IgM, alone or in combination with IL-4). Results indicate that unstimulated splenic B cells show very low E47 protein levels as well as E47 DNA-binding activity and that, upon B cell activation, E47 expression and DNA-binding activity are strongly induced in young and, to a significantly lesser extent, in old mice. The level of E47 protein expression in stimulated splenic B cells was found significantly higher in young than in old mice, suggesting that DNA-binding activity correlates with protein expression. These results altogether suggest that the reduced expression of the transcriptional regulator E47 could help explain the reduced B cell functions in aging mice.
KW - Aging
KW - B lymphocytes
KW - E47 transcription factor
KW - Mouse
KW - Spleen
UR - http://www.scopus.com/inward/record.url?scp=0037396302&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037396302&partnerID=8YFLogxK
U2 - 10.1016/S0047-6374(03)00009-5
DO - 10.1016/S0047-6374(03)00009-5
M3 - Article
C2 - 12714241
AN - SCOPUS:0037396302
VL - 124
SP - 361
EP - 369
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
SN - 0047-6374
IS - 4
ER -