Abstract
In the present paper, we have investigated the effects of aging on the expression and function of the E2A-encoded transcription factor E47 in splenic B lymphocytes, unactivated or activated with different stimuli (LPS, anti-CD40, anti-IgM, alone or in combination with IL-4). Results indicate that unstimulated splenic B cells show very low E47 protein levels as well as E47 DNA-binding activity and that, upon B cell activation, E47 expression and DNA-binding activity are strongly induced in young and, to a significantly lesser extent, in old mice. The level of E47 protein expression in stimulated splenic B cells was found significantly higher in young than in old mice, suggesting that DNA-binding activity correlates with protein expression. These results altogether suggest that the reduced expression of the transcriptional regulator E47 could help explain the reduced B cell functions in aging mice.
Original language | English (US) |
---|---|
Pages (from-to) | 361-369 |
Number of pages | 9 |
Journal | Mechanisms of Ageing and Development |
Volume | 124 |
Issue number | 4 |
DOIs | |
State | Published - Apr 1 2003 |
Keywords
- Aging
- B lymphocytes
- E47 transcription factor
- Mouse
- Spleen
ASJC Scopus subject areas
- Aging
- Biochemistry
- Developmental Biology
- Developmental Neuroscience