Effects of aging on DNA-binding activity of the E47 transcription factor in splenic B cells

Daniela Frasca; Diep Nguyen; Richard L. Riley; Bonnie B. Blomberg

doi:10.1016/j.mad.2003.11.004

Effects of aging on DNA-binding activity of the E47 transcription factor in splenic B cells

Daniela Frasca, Diep Nguyen, Richard L. Riley, Bonnie B. Blomberg

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

In the present paper, we have investigated the DNA-binding activity of the E2A-encoded transcription factor E47 in LPS-activated splenic B cells from young and old BALB/c mice. E47 is a key regulator of B cell differentiation and function: it binds to the E-box site, found in the regulatory regions of several B cell-specific genes (immunoglobulin (Ig), mb-1, and λ5), promotes cell survival of early pre-B cells, helps to initiate Ig rearrangements, promotes class switch and is perhaps involved in somatic mutation in mature splenic and lymph node B cells. Results show that LPS-stimulated splenic B cells from old mice display decreased E47 DNA-binding activity as compared to young mice and that in splenic activated B cells only E47 homodimers bind DNA.

Original language	English (US)
Pages (from-to)	111-112
Number of pages	2
Journal	Mechanisms of Ageing and Development
Volume	125
Issue number	2
DOIs	https://doi.org/10.1016/j.mad.2003.11.004
State	Published - Feb 2004

Keywords

Aging
E47 transcription factor
Splenic B cells

ASJC Scopus subject areas

Aging
Biochemistry
Developmental Biology
Developmental Neuroscience

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title = "Effects of aging on DNA-binding activity of the E47 transcription factor in splenic B cells",

abstract = "In the present paper, we have investigated the DNA-binding activity of the E2A-encoded transcription factor E47 in LPS-activated splenic B cells from young and old BALB/c mice. E47 is a key regulator of B cell differentiation and function: it binds to the E-box site, found in the regulatory regions of several B cell-specific genes (immunoglobulin (Ig), mb-1, and λ5), promotes cell survival of early pre-B cells, helps to initiate Ig rearrangements, promotes class switch and is perhaps involved in somatic mutation in mature splenic and lymph node B cells. Results show that LPS-stimulated splenic B cells from old mice display decreased E47 DNA-binding activity as compared to young mice and that in splenic activated B cells only E47 homodimers bind DNA.",

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AU - Nguyen, Diep

AU - Riley, Richard L.

AU - Blomberg, Bonnie B.

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AB - In the present paper, we have investigated the DNA-binding activity of the E2A-encoded transcription factor E47 in LPS-activated splenic B cells from young and old BALB/c mice. E47 is a key regulator of B cell differentiation and function: it binds to the E-box site, found in the regulatory regions of several B cell-specific genes (immunoglobulin (Ig), mb-1, and λ5), promotes cell survival of early pre-B cells, helps to initiate Ig rearrangements, promotes class switch and is perhaps involved in somatic mutation in mature splenic and lymph node B cells. Results show that LPS-stimulated splenic B cells from old mice display decreased E47 DNA-binding activity as compared to young mice and that in splenic activated B cells only E47 homodimers bind DNA.

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