Abstract
In the present paper, we have investigated the DNA-binding activity of the E2A-encoded transcription factor E47 in LPS-activated splenic B cells from young and old BALB/c mice. E47 is a key regulator of B cell differentiation and function: it binds to the E-box site, found in the regulatory regions of several B cell-specific genes (immunoglobulin (Ig), mb-1, and λ5), promotes cell survival of early pre-B cells, helps to initiate Ig rearrangements, promotes class switch and is perhaps involved in somatic mutation in mature splenic and lymph node B cells. Results show that LPS-stimulated splenic B cells from old mice display decreased E47 DNA-binding activity as compared to young mice and that in splenic activated B cells only E47 homodimers bind DNA.
| Original language | English |
|---|---|
| Pages (from-to) | 111-112 |
| Number of pages | 2 |
| Journal | Mechanisms of Ageing and Development |
| Volume | 125 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2004 |
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Keywords
- Aging
- E47 transcription factor
- Splenic B cells
ASJC Scopus subject areas
- Aging
- Biochemistry
- Developmental Biology
- Developmental Neuroscience
Cite this
Effects of aging on DNA-binding activity of the E47 transcription factor in splenic B cells. / Frasca, Daniela; Nguyen, Diep; Riley, Richard L; Blomberg, Bonnie B.
In: Mechanisms of Ageing and Development, Vol. 125, No. 2, 01.02.2004, p. 111-112.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of aging on DNA-binding activity of the E47 transcription factor in splenic B cells
AU - Frasca, Daniela
AU - Nguyen, Diep
AU - Riley, Richard L
AU - Blomberg, Bonnie B
PY - 2004/2/1
Y1 - 2004/2/1
N2 - In the present paper, we have investigated the DNA-binding activity of the E2A-encoded transcription factor E47 in LPS-activated splenic B cells from young and old BALB/c mice. E47 is a key regulator of B cell differentiation and function: it binds to the E-box site, found in the regulatory regions of several B cell-specific genes (immunoglobulin (Ig), mb-1, and λ5), promotes cell survival of early pre-B cells, helps to initiate Ig rearrangements, promotes class switch and is perhaps involved in somatic mutation in mature splenic and lymph node B cells. Results show that LPS-stimulated splenic B cells from old mice display decreased E47 DNA-binding activity as compared to young mice and that in splenic activated B cells only E47 homodimers bind DNA.
AB - In the present paper, we have investigated the DNA-binding activity of the E2A-encoded transcription factor E47 in LPS-activated splenic B cells from young and old BALB/c mice. E47 is a key regulator of B cell differentiation and function: it binds to the E-box site, found in the regulatory regions of several B cell-specific genes (immunoglobulin (Ig), mb-1, and λ5), promotes cell survival of early pre-B cells, helps to initiate Ig rearrangements, promotes class switch and is perhaps involved in somatic mutation in mature splenic and lymph node B cells. Results show that LPS-stimulated splenic B cells from old mice display decreased E47 DNA-binding activity as compared to young mice and that in splenic activated B cells only E47 homodimers bind DNA.
KW - Aging
KW - E47 transcription factor
KW - Splenic B cells
UR - http://www.scopus.com/inward/record.url?scp=0842281257&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0842281257&partnerID=8YFLogxK
U2 - 10.1016/j.mad.2003.11.004
DO - 10.1016/j.mad.2003.11.004
M3 - Article
C2 - 15037012
AN - SCOPUS:0842281257
VL - 125
SP - 111
EP - 112
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
SN - 0047-6374
IS - 2
ER -