Effects of aging on DNA-binding activity of the E47 transcription factor in splenic B cells

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Abstract

In the present paper, we have investigated the DNA-binding activity of the E2A-encoded transcription factor E47 in LPS-activated splenic B cells from young and old BALB/c mice. E47 is a key regulator of B cell differentiation and function: it binds to the E-box site, found in the regulatory regions of several B cell-specific genes (immunoglobulin (Ig), mb-1, and λ5), promotes cell survival of early pre-B cells, helps to initiate Ig rearrangements, promotes class switch and is perhaps involved in somatic mutation in mature splenic and lymph node B cells. Results show that LPS-stimulated splenic B cells from old mice display decreased E47 DNA-binding activity as compared to young mice and that in splenic activated B cells only E47 homodimers bind DNA.

Original languageEnglish
Pages (from-to)111-112
Number of pages2
JournalMechanisms of Ageing and Development
Volume125
Issue number2
DOIs
StatePublished - Feb 1 2004

Fingerprint

B-Lymphocytes
Transcription Factors
Aging of materials
Cells
DNA
E-Box Elements
Immunoglobulins
Immunoglobulin Genes
B-Lymphoid Precursor Cells
Nucleic Acid Regulatory Sequences
Cell Differentiation
Cell Survival
Lymph Nodes
Mutation
Genes
Switches

Keywords

  • Aging
  • E47 transcription factor
  • Splenic B cells

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

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abstract = "In the present paper, we have investigated the DNA-binding activity of the E2A-encoded transcription factor E47 in LPS-activated splenic B cells from young and old BALB/c mice. E47 is a key regulator of B cell differentiation and function: it binds to the E-box site, found in the regulatory regions of several B cell-specific genes (immunoglobulin (Ig), mb-1, and λ5), promotes cell survival of early pre-B cells, helps to initiate Ig rearrangements, promotes class switch and is perhaps involved in somatic mutation in mature splenic and lymph node B cells. Results show that LPS-stimulated splenic B cells from old mice display decreased E47 DNA-binding activity as compared to young mice and that in splenic activated B cells only E47 homodimers bind DNA.",
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AU - Nguyen, Diep

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AU - Blomberg, Bonnie B

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AB - In the present paper, we have investigated the DNA-binding activity of the E2A-encoded transcription factor E47 in LPS-activated splenic B cells from young and old BALB/c mice. E47 is a key regulator of B cell differentiation and function: it binds to the E-box site, found in the regulatory regions of several B cell-specific genes (immunoglobulin (Ig), mb-1, and λ5), promotes cell survival of early pre-B cells, helps to initiate Ig rearrangements, promotes class switch and is perhaps involved in somatic mutation in mature splenic and lymph node B cells. Results show that LPS-stimulated splenic B cells from old mice display decreased E47 DNA-binding activity as compared to young mice and that in splenic activated B cells only E47 homodimers bind DNA.

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