Abstract
In the present paper, we have investigated the DNA-binding activity of the E2A-encoded transcription factor E47 in LPS-activated splenic B cells from young and old BALB/c mice. E47 is a key regulator of B cell differentiation and function: it binds to the E-box site, found in the regulatory regions of several B cell-specific genes (immunoglobulin (Ig), mb-1, and λ5), promotes cell survival of early pre-B cells, helps to initiate Ig rearrangements, promotes class switch and is perhaps involved in somatic mutation in mature splenic and lymph node B cells. Results show that LPS-stimulated splenic B cells from old mice display decreased E47 DNA-binding activity as compared to young mice and that in splenic activated B cells only E47 homodimers bind DNA.
Original language | English (US) |
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Pages (from-to) | 111-112 |
Number of pages | 2 |
Journal | Mechanisms of Ageing and Development |
Volume | 125 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2004 |
Keywords
- Aging
- E47 transcription factor
- Splenic B cells
ASJC Scopus subject areas
- Aging
- Biochemistry
- Developmental Biology
- Developmental Neuroscience