Abstract
Primary cultures from neonatal rat hearts consist of morphologically distinguishable cardiac muscle and non-muscle cells. The relative fluorescence intensity and nucleolar effects of Adriamycin (ADR) have been studied in these different cell types by fluorescence and phase-contrast microscopy. In cultures exposed to ADR (10 μg/ml) for 30 min, or continuously for 24 h, the intensity of drug-specific fluorescence was significantly greater in the nuclei of muscle, as compared to non-muscle cells. Consistent with these differences in cytofluorescence, nucleolar fragmentation was observed in muscle cells 24 h after a 30-min exposure to ADR, whereas non-muscle cell nucleoli remained intact. With N-trifluoroacetyladriamycin-14-valerate (AD 32), a potent ADR analog, fluorescence was observed in the cytoplasm of both muscle and non-muscle cells; no difference in the intensity of fluorescence between these two cell types was detected. Based on these observations, we believe that drug-induced nucleolar fragmentation in cardiac muscle cells results from higher ADR levels than those achieved in non-muscle cells, and that these differences may have relevance with respect to anthracycline-induced cardiotoxicity in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 913-924 |
| Number of pages | 12 |
| Journal | Journal of Molecular and Cellular Cardiology |
| Volume | 13 |
| Issue number | 10 |
| DOIs | |
| State | Published - Jan 1 1981 |
| Externally published | Yes |
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Keywords
- AD 32
- Adriamycin
- Cardiotoxicity
ASJC Scopus subject areas
- Molecular Biology
- Cardiology and Cardiovascular Medicine
Cite this
Effects of Adriamycin on rat heart cells in culture : Increased accumulation and nucleoli fragmentation in cardiac muscle v. non-muscle cells. / Lampidis, Theodore; Johnson, Lincoln V.; Israel, Mervyn.
In: Journal of Molecular and Cellular Cardiology, Vol. 13, No. 10, 01.01.1981, p. 913-924.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of Adriamycin on rat heart cells in culture
T2 - Increased accumulation and nucleoli fragmentation in cardiac muscle v. non-muscle cells
AU - Lampidis, Theodore
AU - Johnson, Lincoln V.
AU - Israel, Mervyn
PY - 1981/1/1
Y1 - 1981/1/1
N2 - Primary cultures from neonatal rat hearts consist of morphologically distinguishable cardiac muscle and non-muscle cells. The relative fluorescence intensity and nucleolar effects of Adriamycin (ADR) have been studied in these different cell types by fluorescence and phase-contrast microscopy. In cultures exposed to ADR (10 μg/ml) for 30 min, or continuously for 24 h, the intensity of drug-specific fluorescence was significantly greater in the nuclei of muscle, as compared to non-muscle cells. Consistent with these differences in cytofluorescence, nucleolar fragmentation was observed in muscle cells 24 h after a 30-min exposure to ADR, whereas non-muscle cell nucleoli remained intact. With N-trifluoroacetyladriamycin-14-valerate (AD 32), a potent ADR analog, fluorescence was observed in the cytoplasm of both muscle and non-muscle cells; no difference in the intensity of fluorescence between these two cell types was detected. Based on these observations, we believe that drug-induced nucleolar fragmentation in cardiac muscle cells results from higher ADR levels than those achieved in non-muscle cells, and that these differences may have relevance with respect to anthracycline-induced cardiotoxicity in vivo.
AB - Primary cultures from neonatal rat hearts consist of morphologically distinguishable cardiac muscle and non-muscle cells. The relative fluorescence intensity and nucleolar effects of Adriamycin (ADR) have been studied in these different cell types by fluorescence and phase-contrast microscopy. In cultures exposed to ADR (10 μg/ml) for 30 min, or continuously for 24 h, the intensity of drug-specific fluorescence was significantly greater in the nuclei of muscle, as compared to non-muscle cells. Consistent with these differences in cytofluorescence, nucleolar fragmentation was observed in muscle cells 24 h after a 30-min exposure to ADR, whereas non-muscle cell nucleoli remained intact. With N-trifluoroacetyladriamycin-14-valerate (AD 32), a potent ADR analog, fluorescence was observed in the cytoplasm of both muscle and non-muscle cells; no difference in the intensity of fluorescence between these two cell types was detected. Based on these observations, we believe that drug-induced nucleolar fragmentation in cardiac muscle cells results from higher ADR levels than those achieved in non-muscle cells, and that these differences may have relevance with respect to anthracycline-induced cardiotoxicity in vivo.
KW - AD 32
KW - Adriamycin
KW - Cardiotoxicity
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U2 - 10.1016/0022-2828(81)90290-X
DO - 10.1016/0022-2828(81)90290-X
M3 - Article
VL - 13
SP - 913
EP - 924
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
SN - 0022-2828
IS - 10
ER -