Effects of a topical aqueous oxygen emulsion on collagen deposition and angiogenesis in a porcine deep partial-thickness wound model

Jie Li, Jose Ollague Sierra, Linjian Zhu, Ling Tang, Kirah Rahill, Bassim El-Sabawi, Wen Liu-Mares, Patricia M. Mertz, Stephen C Davis

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A porcine deep partial-thickness wound model was used to evaluate the effects of a newly developed topical aqueous oxygen emulsion (TOE) on wound repair. The wounds were treated with TOE, which contains super-saturated oxygen or vehicle control. Semiquantitative immunofluorescent staining was performed to examine protein production for type I and type III collagen and vascular endothelial growth factor (VEGF). Immunofluorescent staining revealed higher protein levels of type I and type III collagen and VEGF in the TOE treatment group. Histological analysis also revealed improved angiogenesis and granulation tissue formation with topical TOE treatment and was consistent with the protein expression. In addition, the histology examination demonstrated faster epithelialization in wounds treated with TOE. The study suggests that sustained high levels of oxygen released by TOE may promote the process of wound repair through increasing collagen deposition and angiogenesis as well as stimulating epithelialization.

Original languageEnglish
Pages (from-to)674-676
Number of pages3
JournalExperimental Dermatology
Volume22
Issue number10
DOIs
StatePublished - Oct 1 2013

Fingerprint

Emulsions
Swine
Collagen
Oxygen
Wounds and Injuries
Collagen Type III
Collagen Type I
Vascular Endothelial Growth Factor A
Repair
Staining and Labeling
Re-Epithelialization
Proteins
Histology
Granulation
Granulation Tissue
Tissue
Therapeutics

Keywords

  • Collagen
  • Granulation tissue formation
  • Partial-thickness wound
  • Topic aqueous oxygen emulsion
  • VEGF

ASJC Scopus subject areas

  • Dermatology
  • Molecular Biology
  • Biochemistry

Cite this

Effects of a topical aqueous oxygen emulsion on collagen deposition and angiogenesis in a porcine deep partial-thickness wound model. / Li, Jie; Ollague Sierra, Jose; Zhu, Linjian; Tang, Ling; Rahill, Kirah; El-Sabawi, Bassim; Liu-Mares, Wen; Mertz, Patricia M.; Davis, Stephen C.

In: Experimental Dermatology, Vol. 22, No. 10, 01.10.2013, p. 674-676.

Research output: Contribution to journalArticle

Li, Jie ; Ollague Sierra, Jose ; Zhu, Linjian ; Tang, Ling ; Rahill, Kirah ; El-Sabawi, Bassim ; Liu-Mares, Wen ; Mertz, Patricia M. ; Davis, Stephen C. / Effects of a topical aqueous oxygen emulsion on collagen deposition and angiogenesis in a porcine deep partial-thickness wound model. In: Experimental Dermatology. 2013 ; Vol. 22, No. 10. pp. 674-676.
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