Effects of a Chinese herbal medicine, Keishi-bukuryogan, on the gonadal system of rats

Sakamoto Shinobu, Kudo Hideki, Kawasaki Tohru, Kuwa Katsuhiko, Noriyuki Kasahara, Sassa Shuji, Okamoto Ryohei

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Keishi-bukuryo-gan (TJ-25) is a traditional Chinese herbal remedy containing five components: bark of Cinnamomum cassia, root of Poeerato lactiflora, seed of Prunus persica or P. persiba var. davidiana, carpophores of Porta cocos and root bark of Paeonia suffruticosa. This preparation has been used in the treatment of gynecological disorders such as hypermenorrhea, dysmenorrhea and infertility. In the present study, the effects of TJ-25 on plasma levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and estradiol (E2), and on uterine wet weight and thymidine kinase (TK) activity were documented in immature rats. Long-term daily oral administration of TJ-25 (300 mg/kg) for 14 days decreased plasma levels of LH, FSH and E2 by 94%, 67% and 50%, respectively, compared to controls. Uterine wet weight and TK activity were reduced to 65% and 64% that of controls, respectively. Short-term effects of TJ-25 on E2 were also examined. Thirty hours after administration of E2 (1.0 μg/kg) alone, uterine wet weight and TK activity were elevated 2.4- and 21-fold, respectively, over controls. However, simultaneous administration of TJ-25 (three consecutive doses, every 12 h) with E2 reduced E2-induced increases in uterine wet weight and TK activity by 29% and 39%, respectively. Treatment with TJ-25 also enhanced LH-RH-induced increases in plasma LH and FSH levels 1.2- and 2.5-fold, respectively, as compared with controls. The results obtained in the present study indicate that TJ-25 may act as a LH-RH antagonist and/or as a weak anti-estrogen.

Original languageEnglish (US)
Pages (from-to)151-158
Number of pages8
JournalJournal of Ethnopharmacology
Volume23
Issue number2-3
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Herbal Medicine
Luteinizing Hormone
Thymidine Kinase
Follicle Stimulating Hormone
Weights and Measures
Cinnamomum aromaticum
Paeonia
Hormone Antagonists
Menorrhagia
Dysmenorrhea
Cocos
keishibukuryogan
Infertility
Oral Administration
Estradiol
Seeds
Estrogens
Therapeutics

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effects of a Chinese herbal medicine, Keishi-bukuryogan, on the gonadal system of rats. / Shinobu, Sakamoto; Hideki, Kudo; Tohru, Kawasaki; Katsuhiko, Kuwa; Kasahara, Noriyuki; Shuji, Sassa; Ryohei, Okamoto.

In: Journal of Ethnopharmacology, Vol. 23, No. 2-3, 1988, p. 151-158.

Research output: Contribution to journalArticle

Shinobu, Sakamoto ; Hideki, Kudo ; Tohru, Kawasaki ; Katsuhiko, Kuwa ; Kasahara, Noriyuki ; Shuji, Sassa ; Ryohei, Okamoto. / Effects of a Chinese herbal medicine, Keishi-bukuryogan, on the gonadal system of rats. In: Journal of Ethnopharmacology. 1988 ; Vol. 23, No. 2-3. pp. 151-158.
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abstract = "Keishi-bukuryo-gan (TJ-25) is a traditional Chinese herbal remedy containing five components: bark of Cinnamomum cassia, root of Poeerato lactiflora, seed of Prunus persica or P. persiba var. davidiana, carpophores of Porta cocos and root bark of Paeonia suffruticosa. This preparation has been used in the treatment of gynecological disorders such as hypermenorrhea, dysmenorrhea and infertility. In the present study, the effects of TJ-25 on plasma levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and estradiol (E2), and on uterine wet weight and thymidine kinase (TK) activity were documented in immature rats. Long-term daily oral administration of TJ-25 (300 mg/kg) for 14 days decreased plasma levels of LH, FSH and E2 by 94{\%}, 67{\%} and 50{\%}, respectively, compared to controls. Uterine wet weight and TK activity were reduced to 65{\%} and 64{\%} that of controls, respectively. Short-term effects of TJ-25 on E2 were also examined. Thirty hours after administration of E2 (1.0 μg/kg) alone, uterine wet weight and TK activity were elevated 2.4- and 21-fold, respectively, over controls. However, simultaneous administration of TJ-25 (three consecutive doses, every 12 h) with E2 reduced E2-induced increases in uterine wet weight and TK activity by 29{\%} and 39{\%}, respectively. Treatment with TJ-25 also enhanced LH-RH-induced increases in plasma LH and FSH levels 1.2- and 2.5-fold, respectively, as compared with controls. The results obtained in the present study indicate that TJ-25 may act as a LH-RH antagonist and/or as a weak anti-estrogen.",
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T1 - Effects of a Chinese herbal medicine, Keishi-bukuryogan, on the gonadal system of rats

AU - Shinobu, Sakamoto

AU - Hideki, Kudo

AU - Tohru, Kawasaki

AU - Katsuhiko, Kuwa

AU - Kasahara, Noriyuki

AU - Shuji, Sassa

AU - Ryohei, Okamoto

PY - 1988

Y1 - 1988

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