Effects of 0.5 ppm ozone on glycoprotein secretion, ion and water fluxes in sheep trachea

R. J. Phipps, S. M. Denas, M. W. Sielczak, Adam Wanner

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Abstract

We studied the effects of ozone (O3) exposure on airway mucus secretion. Sheep were exposed in vivo to 0.5 ppm O3, 4 h/day for 2 days (acute, n = 6), 6 wks (chronic, n = 6) or 6 wks + 1 wk recovery (chronic + recovery, n = 6). Secretion of glycoproteins (radiolabeled with 35SO4 and [3H]threonine), and transepithelial fluxes of Cl-, Na+ and water were subsequently measured in tracheal tissues in vitro, and were compared with values from control, unexposed sheep (n = 8). Acute O3 exposure increased basal secretion of sulfated glycoproteins (P < 0.05), but had no effect on ion fluxes. Chronic exposure reduced basal glycoprotein secretion, but increased net Cl- secretion. Under open-circuit conditions, chronic exposure also induced net water secretion (P < 0.05). With 7 days recovery, basal glycoprotein secretion (predominantly sulfated) was greatly increased above control, while the increased net secretion of Cl- and of water persisted (P < 0.05). Histology of the airways indicated that acute exposure induced moderate hypertrophy of submucosal glands in the lower trachea (P < 0.05), while chronic exposure (with and without recovery) induced a large hypertrophy of submucosal glands in both upper and lower trachea (P < 0.05). Without recovery, however, the gland cells were devoid of secretory material, whereas with recovery they were full of secretory material. This suggests that the decreased glycoprotein secretion with chronic exposure alone resulted from incomplete replenishment of intracellular stores after 6 wks of stimulation. We conclude that both short- and long-term O3 exposure causes airway-mucus hypersecretion.

Original languageEnglish
Pages (from-to)918-927
Number of pages10
JournalJournal of Applied Physiology
Volume60
Issue number3
StatePublished - Jan 1 1986

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Ozone
Trachea
Sheep
Glycoproteins
Ions
Water
Mucus
Hypertrophy
P-Glycoproteins
Threonine
Histology

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Effects of 0.5 ppm ozone on glycoprotein secretion, ion and water fluxes in sheep trachea. / Phipps, R. J.; Denas, S. M.; Sielczak, M. W.; Wanner, Adam.

In: Journal of Applied Physiology, Vol. 60, No. 3, 01.01.1986, p. 918-927.

Research output: Contribution to journalArticle

Phipps, R. J. ; Denas, S. M. ; Sielczak, M. W. ; Wanner, Adam. / Effects of 0.5 ppm ozone on glycoprotein secretion, ion and water fluxes in sheep trachea. In: Journal of Applied Physiology. 1986 ; Vol. 60, No. 3. pp. 918-927.
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