Effects of γ-interferon and tumor necrosis factor α on thyroid cells: induction of class II antigen and inhibition of growth stimulation

M. Zakarija, F. J. Hornicek, S. Levis, J. M. McKenzie

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

A functioning rat thyroid cell line (FRTL5) was used to study interactions of thyrotropin (TSH) and various cytokines on expression of class I and II major histocompatibility complex (MHC) antigens and on growth stimulation. Only γ-interferon (γ-IFN) affected MHC antigen expression, i.e., to enhance class I. that was constitutive, and to induce class II. A concomitant, but probably not directly related, effect of γ-IFN was to diminish growth stimulation, as effected by TSH and other activators of adenylate cyclase and measured by DNA increase and enhanced incorporation of [3H]thymidine into DNA. Stimulation of growth by tetradecanoylphorbol ester was also decreased by γ-IFN. These effects of γ-IFN were mimicked to some degree by tumor necrosis factor but there was major synergism between the two cytokines. Enhanced accumulation of cAMP by TSH and other agents was not diminished in these experiments. Flow cytometry analysis showed that inhibition of growth stimulation involved blocking of the passage of cells from the g 0 1 phase to the S phase. The data may have relevance to goiter size in autoimmune thyroid disease.

Original languageEnglish (US)
Pages (from-to)129-136
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume58
Issue number2-3
DOIs
StatePublished - Aug 1988

Keywords

  • Class II antigen
  • Interferon-γ
  • Thyroid
  • Thyrotrophin
  • Tumor necrosis factor α

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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