Effective use of frozen donor peripheral blood mononuclear cells for human immunodeficiency virus type 1 isolation from vertically infected pediatric patients

M. O. Paul; S. Tetali; S. Pahwa

doi:10.1128/jcm.32.5.1379-1382.1994

Effective use of frozen donor peripheral blood mononuclear cells for human immunodeficiency virus type 1 isolation from vertically infected pediatric patients

M. O. Paul, S. Tetali, S. Pahwa

Research output: Contribution to journal › Comment/debate › peer-review

3 Scopus citations

Abstract

In this study, we examined variables related to human immunodeficiency virus (HIV) isolation utilizing samples from 51 HIV-infected (153 plasma and 122 peripheral blood mononuclear cell [PBMC] samples) and 57 uninfected (182 plasma and 163 PBMC samples) infants. Our chief observation was that cryopreservation of donor PBMCs does not significantly alter their sensitivity or specificity for isolation of HIV from patient PBMCs or plasma.

Original language	English (US)
Pages (from-to)	1379-1382
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	32
Issue number	5
DOIs	https://doi.org/10.1128/jcm.32.5.1379-1382.1994
State	Published - 1994
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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abstract = "In this study, we examined variables related to human immunodeficiency virus (HIV) isolation utilizing samples from 51 HIV-infected (153 plasma and 122 peripheral blood mononuclear cell [PBMC] samples) and 57 uninfected (182 plasma and 163 PBMC samples) infants. Our chief observation was that cryopreservation of donor PBMCs does not significantly alter their sensitivity or specificity for isolation of HIV from patient PBMCs or plasma.",

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AU - Paul, M. O.

AU - Tetali, S.

AU - Pahwa, S.

PY - 1994

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N2 - In this study, we examined variables related to human immunodeficiency virus (HIV) isolation utilizing samples from 51 HIV-infected (153 plasma and 122 peripheral blood mononuclear cell [PBMC] samples) and 57 uninfected (182 plasma and 163 PBMC samples) infants. Our chief observation was that cryopreservation of donor PBMCs does not significantly alter their sensitivity or specificity for isolation of HIV from patient PBMCs or plasma.

AB - In this study, we examined variables related to human immunodeficiency virus (HIV) isolation utilizing samples from 51 HIV-infected (153 plasma and 122 peripheral blood mononuclear cell [PBMC] samples) and 57 uninfected (182 plasma and 163 PBMC samples) infants. Our chief observation was that cryopreservation of donor PBMCs does not significantly alter their sensitivity or specificity for isolation of HIV from patient PBMCs or plasma.

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U2 - 10.1128/jcm.32.5.1379-1382.1994

DO - 10.1128/jcm.32.5.1379-1382.1994

M3 - Comment/debate

C2 - 8051275

AN - SCOPUS:0028289407

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JO - Journal of Clinical Microbiology

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