Effective small interfering RNAs and phosphorothioate antisense DNAs have different preferences for target sites in the luciferase mRNAs

Yunhe Xu, Hong Yan Zhang, Dorit Thormeyer, Ola Larsson, Quan Du, Joacim Elmén, Claes Wahlestedt, Zicai Liang

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

Antisense DNA target sites can be selected by the accessibility of the mRNA target. It remains unknown whether a mRNA site that is accessible to an antisense DNA is also a good candidate target site for a siRNA. Here, we reported a parallel analysis of 12 pairs of antisense DNAs and siRNA duplexes for their potency to inhibit reporter luciferase activity in mammalian cells, both of the antisense DNA and siRNA agents in a pair being directed to same site in the mRNA. Five siRNAs and two antisense DNAs turned out to be effective, but the sites targeted by those effective siRNAs and antisense DNAs did not overlap. Our results indicated that effective antisense DNAs and siRNAs have different preferences for target sites in the mRNA.

Original languageEnglish (US)
Pages (from-to)712-717
Number of pages6
JournalBiochemical and biophysical research communications
Volume306
Issue number3
DOIs
StatePublished - Jul 4 2003
Externally publishedYes

Keywords

  • Antisense DNAs
  • mRNA
  • Small interfering RNAs

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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