Effect on growth and cell cycle kinetics of estradiol and tamoxifen on MCF-7 human breast cancer cells grown in vitro and in nude mice

N. Brunner, D. Bronzert, L. L. Vindelov, K. Rygaard, M. Spang-Thomsen, Marc E Lippman

Research output: Contribution to journalArticle

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Abstract

The effects of estradiol and tamoxifen (TAM) on the estrogen-dependent human breast cancer cell line MCF-7 grown in vitro and in nude mice were compared. The effect on growth was determined by cell number in vitro and by tumor growth curves in nude mice. The effects on the cell cycle kinetics were determined by repeated flow cytometric DNA analyses in vitro and in vivo and by the technique of labeled mitosis in nude mouse-grown tumors. Under in vitro conditions, estradiol induced a pronounced increase in S-phase fraction and cell number. TAM inhibited growth of MCF-7 cells with a concomitant increase in the G1 phase from 60% to 75%. In nude mice, MCF-7 only formed tumors in estradiol-supplemented mice. No differences were observed in growth and cell kinetics between 0.1 and 1.0 mg of estradiol. Daily i.p. injections of TAM resulted in tumor growth inhibition with shrinkage of tumors. The flow cytometric DNA analysis and percentage of labeled mitosis investigations revealed no significant differences in the proliferation kinetics of TAM-treated and control tumors. Calculating the cell loss factor demonstrated an increase from 69% in control tumors to 107% in TAM-treated tumors. These experiments have shown that the cell kinetic effect of TAM is different when MCF-7 cells are grown in vitro versus in vivo. In contrast to the in vitro data, the in vivo data indicate that the growth-inhibitory effect of TAM is not mediated through a perturbation of the cell cycle.

Original languageEnglish
Pages (from-to)1515-1520
Number of pages6
JournalCancer Research
Volume49
Issue number6
StatePublished - Jan 1 1989
Externally publishedYes

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Tamoxifen
Nude Mice
Estradiol
Cell Cycle
Breast Neoplasms
Growth
Neoplasms
MCF-7 Cells
Mitosis
Cell Count
In Vitro Techniques
DNA
G1 Phase
S Phase
Estrogens
Cell Line
Injections

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Brunner, N., Bronzert, D., Vindelov, L. L., Rygaard, K., Spang-Thomsen, M., & Lippman, M. E. (1989). Effect on growth and cell cycle kinetics of estradiol and tamoxifen on MCF-7 human breast cancer cells grown in vitro and in nude mice. Cancer Research, 49(6), 1515-1520.

Effect on growth and cell cycle kinetics of estradiol and tamoxifen on MCF-7 human breast cancer cells grown in vitro and in nude mice. / Brunner, N.; Bronzert, D.; Vindelov, L. L.; Rygaard, K.; Spang-Thomsen, M.; Lippman, Marc E.

In: Cancer Research, Vol. 49, No. 6, 01.01.1989, p. 1515-1520.

Research output: Contribution to journalArticle

Brunner, N, Bronzert, D, Vindelov, LL, Rygaard, K, Spang-Thomsen, M & Lippman, ME 1989, 'Effect on growth and cell cycle kinetics of estradiol and tamoxifen on MCF-7 human breast cancer cells grown in vitro and in nude mice', Cancer Research, vol. 49, no. 6, pp. 1515-1520.
Brunner N, Bronzert D, Vindelov LL, Rygaard K, Spang-Thomsen M, Lippman ME. Effect on growth and cell cycle kinetics of estradiol and tamoxifen on MCF-7 human breast cancer cells grown in vitro and in nude mice. Cancer Research. 1989 Jan 1;49(6):1515-1520.
Brunner, N. ; Bronzert, D. ; Vindelov, L. L. ; Rygaard, K. ; Spang-Thomsen, M. ; Lippman, Marc E. / Effect on growth and cell cycle kinetics of estradiol and tamoxifen on MCF-7 human breast cancer cells grown in vitro and in nude mice. In: Cancer Research. 1989 ; Vol. 49, No. 6. pp. 1515-1520.
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