Effect of v-ras(H) oncogene transfection on estrogen-independent tumorigenicity of estrogen-dependent human breast cancer cells

A. Kasid, C. Knabbe, Marc E Lippman

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Spontaneous or therapeutically induced progression of hormone-dependent human breast cancer to a form not amenable to endocrine treatment has been frequently recorded in clinical settings. In an experimental model system, we have changed the estrogen-dependent tumorigenicity of a human breast cancer cell line, MCF-7, to an independent state by stably introducing a model oncogene, v-ras(H), into this cell line by means of DNA transfection. We now show that the oncogene-transfected hormone-independent MCF-7 cells may secrete diffusible tumorigenic factors that not only support their own tumor growth in vivo, but are also humorally active in partially triggering the tumor growth of wild type previously nontumorigenic MCF-7 cells, even when the wild type cells are implanted at a distant anatomical site in the same animal. Estrogen-independent tumor formation by MCF-7 cells was also induced in 50% of animals given injection by continuous administration of conditioned media from MCF-7-ras cells. However, the wild type tumors had limited tumor growth. Tumors were verified as adenocarcinomas and by Southern blotting were shown to be derived from the cells injected. In an in vitro coculture assay, a 5- to 7-fold enhancement in anchorage-independent growth of MCF-7 cells was observed in the presence of MCF-7-ras feeder cell layer. These data suggest that v-ras(H)-induced estrogen-independent tumorigenicity of human breast cancer cells occurs by secretion of mitogens which may function in an endocrine manner.

Original languageEnglish
Pages (from-to)5733-5738
Number of pages6
JournalCancer Research
Volume47
Issue number21
StatePublished - Dec 1 1987
Externally publishedYes

Fingerprint

ras Genes
MCF-7 Cells
Transfection
Estrogens
Breast Neoplasms
Neoplasms
Growth
Hormones
Feeder Cells
Cell Line
Conditioned Culture Medium
Coculture Techniques
Southern Blotting
Mitogens
Oncogenes
Adenocarcinoma
Theoretical Models
Injections
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Effect of v-ras(H) oncogene transfection on estrogen-independent tumorigenicity of estrogen-dependent human breast cancer cells. / Kasid, A.; Knabbe, C.; Lippman, Marc E.

In: Cancer Research, Vol. 47, No. 21, 01.12.1987, p. 5733-5738.

Research output: Contribution to journalArticle

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