Effect of Trp53 gene deficiency on brain injury after neonatal hypoxia-ischemia

Ana A. Baburamani, Kristina S. Sobotka, Regina Vontell, Carina Mallard, Veena G. Supramaniam, Claire Thornton, Henrik Hagberg

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Hypoxia-ischemia (HI) can result in permanent life-long injuries such as motor and cognitive deficits. In response to cellular stressors such as hypoxia, tumor suppressor protein p53 is activated, potently initiating apoptosis and promoting Bax-dependent mitochondrial outer membrane permeabilization. The aim of this study was to investigate the effect of Trp53 genetic inhibition on injury development in the immature brain following HI. HI (50 min or 60 min) was induced at postnatal day 9 (PND9) in Trp53 heterozygote (het) and wild type (WT) mice. Utilizing Cre- LoxP technology, CaMK2a-Cre mice were bred with Trp53-Lox mice, resulting in knockdown of Trp53 in CaMK2a neurons. HI was induced at PND12 (50 min) and PND28 (40 min). Extent of brain injury was assessed 7 days following HI. Following 50 min HI at PND9, Trp53 het mice showed protection in the posterior hippocampus and thalamus. No difference was seen between WT or Trp53 het mice following a severe, 60 min HI. Cre-Lox mice that were subjected to HI at PND12 showed no difference in injury, however we determined that neuronal specific CaMK2a-Cre recombinase activity was strongly expressed by PND28. Concomitantly, Trp53 was reduced at 6 weeks of age in KO-Lox Trp53 mice. Cre-Lox mice subjected to HI at PND28 showed no significant difference in brain injury. These data suggest that p53 has a limited contribution to the development of injury in the immature/juvenile brain following HI. Further studies are required to determine the effect of p53 on downstream targets.

Original languageEnglish (US)
Pages (from-to)12081-12092
Number of pages12
Issue number7
StatePublished - 2017
Externally publishedYes


  • Brain injury
  • Cell death
  • Hypoxia-ischemia
  • Mitochondria
  • p53

ASJC Scopus subject areas

  • Oncology


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