Effect of topical fluoroquinolones on the expression of matrix metalloproteinases in the cornea

Victor E. Reviglio, Melinda A. Hakim, Jae K. Song, Terrence O'Brien

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: Matrix metalloproteinases play an important role in extracellular matrix deposition and degradation. Based on previous clinical observations of corneal perforations during topical fluoroquinolone treatment, we decided to evaluate the comparative effects of various fluoroquinolone eye drops on the expression of matrix metalloproteinases (MMPs) in cornea. Methods: Eighty female Lewis rats were divided into two experimental groups: intact and wounded corneal epithelium. Uniform corneal epithelial defects were created in the right eye with application of 75% alcohol in the center of the tissue for 6 seconds. The treatment groups were tested as follows: 1) Tear drops: carboxymethylcellulose sodium 0.5% (Refresh, Allergan); 2) Ciprofloxacin 0.3% (Ciloxan, Alcon); 3) Ofloxacin 0.3%(Ocuflox, Allergan); 4) Levofloxacin 0.5%(Quixin, Santen). Eye drops were administered 6 times a day for 48 hours. Rats were sacrificed at 48 hours. Immunohistochemical analysis and zymography were conducted using antibodies specific to MMPs-1, 2, 8 and 9. Results: MMP-1, MMP-2, MMP-8 and MMP-9 expression were detected at 48 hrs in undebrided corneal epithelium groups treated with the topical fluoroquinolones. No statistical difference was observed in quantitative expression of MMPs among ciprofloxacin 0.3%, ofloxacin 0.3%, levofloxacin 0.5%. When the artificial tear group and the fluoroquinolone groups with corneal epithelial defect were compared, increased expression of MMPs was observed as a result of the wound healing process. However, the fluoroquinolone treated group exhibited high statistically significantly levels of MMPs expression. Conclusions: Our study provides preliminary evidence that topical application of fluoroquinolone drugs can induce the expression of MMP-1, MMP-2, MMP-8 and MMP-9 in the undebrided corneal epithelium compared to artificial tear eye drops.

Original languageEnglish
Article number1
Pages (from-to)1-8
Number of pages8
JournalBMC Ophthalmology
Volume3
DOIs
StatePublished - Oct 6 2003
Externally publishedYes

Fingerprint

Fluoroquinolones
Matrix Metalloproteinases
Cornea
Corneal Epithelium
Matrix Metalloproteinase 1
Levofloxacin
Ophthalmic Solutions
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Matrix Metalloproteinase 8
Ofloxacin
Ciprofloxacin
Artificial Eye
Corneal Perforation
Carboxymethylcellulose Sodium
Tears
Wound Healing
Extracellular Matrix
Alcohols
Antibodies

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effect of topical fluoroquinolones on the expression of matrix metalloproteinases in the cornea. / Reviglio, Victor E.; Hakim, Melinda A.; Song, Jae K.; O'Brien, Terrence.

In: BMC Ophthalmology, Vol. 3, 1, 06.10.2003, p. 1-8.

Research output: Contribution to journalArticle

Reviglio, Victor E. ; Hakim, Melinda A. ; Song, Jae K. ; O'Brien, Terrence. / Effect of topical fluoroquinolones on the expression of matrix metalloproteinases in the cornea. In: BMC Ophthalmology. 2003 ; Vol. 3. pp. 1-8.
@article{bb735183ff864b7b8030f10d6e67c8e1,
title = "Effect of topical fluoroquinolones on the expression of matrix metalloproteinases in the cornea",
abstract = "Background: Matrix metalloproteinases play an important role in extracellular matrix deposition and degradation. Based on previous clinical observations of corneal perforations during topical fluoroquinolone treatment, we decided to evaluate the comparative effects of various fluoroquinolone eye drops on the expression of matrix metalloproteinases (MMPs) in cornea. Methods: Eighty female Lewis rats were divided into two experimental groups: intact and wounded corneal epithelium. Uniform corneal epithelial defects were created in the right eye with application of 75{\%} alcohol in the center of the tissue for 6 seconds. The treatment groups were tested as follows: 1) Tear drops: carboxymethylcellulose sodium 0.5{\%} (Refresh, Allergan); 2) Ciprofloxacin 0.3{\%} (Ciloxan, Alcon); 3) Ofloxacin 0.3{\%}(Ocuflox, Allergan); 4) Levofloxacin 0.5{\%}(Quixin, Santen). Eye drops were administered 6 times a day for 48 hours. Rats were sacrificed at 48 hours. Immunohistochemical analysis and zymography were conducted using antibodies specific to MMPs-1, 2, 8 and 9. Results: MMP-1, MMP-2, MMP-8 and MMP-9 expression were detected at 48 hrs in undebrided corneal epithelium groups treated with the topical fluoroquinolones. No statistical difference was observed in quantitative expression of MMPs among ciprofloxacin 0.3{\%}, ofloxacin 0.3{\%}, levofloxacin 0.5{\%}. When the artificial tear group and the fluoroquinolone groups with corneal epithelial defect were compared, increased expression of MMPs was observed as a result of the wound healing process. However, the fluoroquinolone treated group exhibited high statistically significantly levels of MMPs expression. Conclusions: Our study provides preliminary evidence that topical application of fluoroquinolone drugs can induce the expression of MMP-1, MMP-2, MMP-8 and MMP-9 in the undebrided corneal epithelium compared to artificial tear eye drops.",
author = "Reviglio, {Victor E.} and Hakim, {Melinda A.} and Song, {Jae K.} and Terrence O'Brien",
year = "2003",
month = "10",
day = "6",
doi = "10.1186/1471-2415-3-1",
language = "English",
volume = "3",
pages = "1--8",
journal = "BMC Ophthalmology",
issn = "1471-2415",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Effect of topical fluoroquinolones on the expression of matrix metalloproteinases in the cornea

AU - Reviglio, Victor E.

AU - Hakim, Melinda A.

AU - Song, Jae K.

AU - O'Brien, Terrence

PY - 2003/10/6

Y1 - 2003/10/6

N2 - Background: Matrix metalloproteinases play an important role in extracellular matrix deposition and degradation. Based on previous clinical observations of corneal perforations during topical fluoroquinolone treatment, we decided to evaluate the comparative effects of various fluoroquinolone eye drops on the expression of matrix metalloproteinases (MMPs) in cornea. Methods: Eighty female Lewis rats were divided into two experimental groups: intact and wounded corneal epithelium. Uniform corneal epithelial defects were created in the right eye with application of 75% alcohol in the center of the tissue for 6 seconds. The treatment groups were tested as follows: 1) Tear drops: carboxymethylcellulose sodium 0.5% (Refresh, Allergan); 2) Ciprofloxacin 0.3% (Ciloxan, Alcon); 3) Ofloxacin 0.3%(Ocuflox, Allergan); 4) Levofloxacin 0.5%(Quixin, Santen). Eye drops were administered 6 times a day for 48 hours. Rats were sacrificed at 48 hours. Immunohistochemical analysis and zymography were conducted using antibodies specific to MMPs-1, 2, 8 and 9. Results: MMP-1, MMP-2, MMP-8 and MMP-9 expression were detected at 48 hrs in undebrided corneal epithelium groups treated with the topical fluoroquinolones. No statistical difference was observed in quantitative expression of MMPs among ciprofloxacin 0.3%, ofloxacin 0.3%, levofloxacin 0.5%. When the artificial tear group and the fluoroquinolone groups with corneal epithelial defect were compared, increased expression of MMPs was observed as a result of the wound healing process. However, the fluoroquinolone treated group exhibited high statistically significantly levels of MMPs expression. Conclusions: Our study provides preliminary evidence that topical application of fluoroquinolone drugs can induce the expression of MMP-1, MMP-2, MMP-8 and MMP-9 in the undebrided corneal epithelium compared to artificial tear eye drops.

AB - Background: Matrix metalloproteinases play an important role in extracellular matrix deposition and degradation. Based on previous clinical observations of corneal perforations during topical fluoroquinolone treatment, we decided to evaluate the comparative effects of various fluoroquinolone eye drops on the expression of matrix metalloproteinases (MMPs) in cornea. Methods: Eighty female Lewis rats were divided into two experimental groups: intact and wounded corneal epithelium. Uniform corneal epithelial defects were created in the right eye with application of 75% alcohol in the center of the tissue for 6 seconds. The treatment groups were tested as follows: 1) Tear drops: carboxymethylcellulose sodium 0.5% (Refresh, Allergan); 2) Ciprofloxacin 0.3% (Ciloxan, Alcon); 3) Ofloxacin 0.3%(Ocuflox, Allergan); 4) Levofloxacin 0.5%(Quixin, Santen). Eye drops were administered 6 times a day for 48 hours. Rats were sacrificed at 48 hours. Immunohistochemical analysis and zymography were conducted using antibodies specific to MMPs-1, 2, 8 and 9. Results: MMP-1, MMP-2, MMP-8 and MMP-9 expression were detected at 48 hrs in undebrided corneal epithelium groups treated with the topical fluoroquinolones. No statistical difference was observed in quantitative expression of MMPs among ciprofloxacin 0.3%, ofloxacin 0.3%, levofloxacin 0.5%. When the artificial tear group and the fluoroquinolone groups with corneal epithelial defect were compared, increased expression of MMPs was observed as a result of the wound healing process. However, the fluoroquinolone treated group exhibited high statistically significantly levels of MMPs expression. Conclusions: Our study provides preliminary evidence that topical application of fluoroquinolone drugs can induce the expression of MMP-1, MMP-2, MMP-8 and MMP-9 in the undebrided corneal epithelium compared to artificial tear eye drops.

UR - http://www.scopus.com/inward/record.url?scp=2942756147&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942756147&partnerID=8YFLogxK

U2 - 10.1186/1471-2415-3-1

DO - 10.1186/1471-2415-3-1

M3 - Article

VL - 3

SP - 1

EP - 8

JO - BMC Ophthalmology

JF - BMC Ophthalmology

SN - 1471-2415

M1 - 1

ER -