Effect of thyroid status on α- and β-catecholamine responsiveness of hamster adipocytes

J. Adolfo Garćia-Sáinz, Irene Litosch, Brian B. Hoffman, Robert J. Lefkowitz, John N. Fain

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


It has been suggested that part of the increased β-catecholamine responsiveness in hyperthyroid animals is due to a decrease in α-catecholamine action. The present results indicate that neither hyperthyroidism nor hypothyroidism altered the α2-adrenergic inhibition of adenylate cyclase or the α1-adrenergic stimulation of phosphatidylinositol turnover in adipocytes from the white adipose tissue of hamster. No effect of hyperthyroidism was found on the Kd of [3H]dihydroegocryptine or the number of binding sites in membranes prepared from hamster adipocyte tissue. The stimulation of cyclic AMP due to β-catecholamines was enhanced in adipocytes from hyperthyroid hamster, as was lipolysis. However, in adipocytes from hyperthyroid hamster the maximal stimulation of cyclic AMP due to isoproterenol, ACTH or epinephrine plus yohimbine, as seen in the presence of adenosine deaminase and theophylline, was less than in adipocytes from euthyroid hamsters. The activation of adenylate cyclase by isoproterenol was the same in membranes from hyperthyroid as compared to those from euthyroid hamsters in the absence or presence of guanine nucleotides. These data suggest that thyroid status has little effect on α-catecholamine action but enhances the activation of lipolysis by β-catecholamine agonists.

Original languageEnglish (US)
Pages (from-to)334-341
Number of pages8
JournalBBA - General Subjects
Issue number3
StatePublished - Dec 18 1981
Externally publishedYes


  • (Hamster adipocyte)
  • Catecholamine responsiveness
  • Thyroid state

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Medicine(all)


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