Effect of the 1,450 nm diode non-ablative laser on collagen expression in an artificial skin model

Keyvan Nouri; Yan Ping Zhang; Lauren Singer; Linjian Zhu; Ran Huo; Carlos Ricotti; Srdjan Prodanovich; Jie Li

doi:10.1002/lsm.20189

Effect of the 1,450 nm diode non-ablative laser on collagen expression in an artificial skin model

Keyvan Nouri, Yan Ping Zhang, Lauren Singer, Linjian Zhu, Ran Huo, Carlos Ricotti, Srdjan Prodanovich, Jie Li

Dermatology & Cutaneous Surgery

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Background and Objective: The 1,450-nm Smooth-beam™ Laser is a diode laser equipped with a cryogen cooling spray. Primary objectives were to evaluate the effects of this non-ablative laser on Apligraf® (bioengineered skin-substitute) and to document its use as a model for non-ablative procedures. We also measured the effects of laser fluence levels on collagen and elastin expression. Study Design/Materials and Methods: Three sheets of Apligraf® were used for this study. Each received six separate laser applications at 4J, 6J, 8J, 10J, 12J, and 14J. The sheets were then incubated with 10% CO ₂ at 37°C and samples were collected and analyzed 3 days later, using RT-PCR and immunofluorescent staining. Results: Collagen III expressions significantly increased in both mRNA and protein levels at approximately 12J. Conclusions: There appears to be a threshold effect where there is very little increased collagen III mRNA and protein expression until the laser fluence reaches around 12J.

Original language	English (US)
Pages (from-to)	97-102
Number of pages	6
Journal	Lasers in Surgery and Medicine
Volume	37
Issue number	1
DOIs	https://doi.org/10.1002/lsm.20189
State	Published - Jul 1 2005

Keywords

Apligraf®
Collagen expression
Non-ablative laser

ASJC Scopus subject areas

Surgery

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10.1002/lsm.20189

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title = "Effect of the 1,450 nm diode non-ablative laser on collagen expression in an artificial skin model",

abstract = "Background and Objective: The 1,450-nm Smooth-beam{\texttrademark} Laser is a diode laser equipped with a cryogen cooling spray. Primary objectives were to evaluate the effects of this non-ablative laser on Apligraf{\textregistered} (bioengineered skin-substitute) and to document its use as a model for non-ablative procedures. We also measured the effects of laser fluence levels on collagen and elastin expression. Study Design/Materials and Methods: Three sheets of Apligraf{\textregistered} were used for this study. Each received six separate laser applications at 4J, 6J, 8J, 10J, 12J, and 14J. The sheets were then incubated with 10% CO 2 at 37°C and samples were collected and analyzed 3 days later, using RT-PCR and immunofluorescent staining. Results: Collagen III expressions significantly increased in both mRNA and protein levels at approximately 12J. Conclusions: There appears to be a threshold effect where there is very little increased collagen III mRNA and protein expression until the laser fluence reaches around 12J.",

keywords = "Apligraf{\textregistered}, Collagen expression, Non-ablative laser",

author = "Keyvan Nouri and Zhang, {Yan Ping} and Lauren Singer and Linjian Zhu and Ran Huo and Carlos Ricotti and Srdjan Prodanovich and Jie Li",

year = "2005",

month = jul,

day = "1",

doi = "10.1002/lsm.20189",

language = "English (US)",

volume = "37",

pages = "97--102",

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T1 - Effect of the 1,450 nm diode non-ablative laser on collagen expression in an artificial skin model

AU - Nouri, Keyvan

AU - Zhang, Yan Ping

AU - Singer, Lauren

AU - Zhu, Linjian

AU - Huo, Ran

AU - Ricotti, Carlos

AU - Prodanovich, Srdjan

AU - Li, Jie

PY - 2005/7/1

Y1 - 2005/7/1

N2 - Background and Objective: The 1,450-nm Smooth-beam™ Laser is a diode laser equipped with a cryogen cooling spray. Primary objectives were to evaluate the effects of this non-ablative laser on Apligraf® (bioengineered skin-substitute) and to document its use as a model for non-ablative procedures. We also measured the effects of laser fluence levels on collagen and elastin expression. Study Design/Materials and Methods: Three sheets of Apligraf® were used for this study. Each received six separate laser applications at 4J, 6J, 8J, 10J, 12J, and 14J. The sheets were then incubated with 10% CO 2 at 37°C and samples were collected and analyzed 3 days later, using RT-PCR and immunofluorescent staining. Results: Collagen III expressions significantly increased in both mRNA and protein levels at approximately 12J. Conclusions: There appears to be a threshold effect where there is very little increased collagen III mRNA and protein expression until the laser fluence reaches around 12J.

AB - Background and Objective: The 1,450-nm Smooth-beam™ Laser is a diode laser equipped with a cryogen cooling spray. Primary objectives were to evaluate the effects of this non-ablative laser on Apligraf® (bioengineered skin-substitute) and to document its use as a model for non-ablative procedures. We also measured the effects of laser fluence levels on collagen and elastin expression. Study Design/Materials and Methods: Three sheets of Apligraf® were used for this study. Each received six separate laser applications at 4J, 6J, 8J, 10J, 12J, and 14J. The sheets were then incubated with 10% CO 2 at 37°C and samples were collected and analyzed 3 days later, using RT-PCR and immunofluorescent staining. Results: Collagen III expressions significantly increased in both mRNA and protein levels at approximately 12J. Conclusions: There appears to be a threshold effect where there is very little increased collagen III mRNA and protein expression until the laser fluence reaches around 12J.

KW - Apligraf®

KW - Collagen expression

KW - Non-ablative laser

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Effect of the 1,450 nm diode non-ablative laser on collagen expression in an artificial skin model

Abstract

Keywords

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