Effect of the 1,450 nm diode non-ablative laser on collagen expression in an artificial skin model

Keyvan Nouri, Yan Ping Zhang, Lauren Singer, Linjian Zhu, Ran Huo, Carlos Ricotti, Srdjan Prodanovich, Jie Li

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Background and Objective: The 1,450-nm Smooth-beam™ Laser is a diode laser equipped with a cryogen cooling spray. Primary objectives were to evaluate the effects of this non-ablative laser on Apligraf® (bioengineered skin-substitute) and to document its use as a model for non-ablative procedures. We also measured the effects of laser fluence levels on collagen and elastin expression. Study Design/Materials and Methods: Three sheets of Apligraf® were used for this study. Each received six separate laser applications at 4J, 6J, 8J, 10J, 12J, and 14J. The sheets were then incubated with 10% CO 2 at 37°C and samples were collected and analyzed 3 days later, using RT-PCR and immunofluorescent staining. Results: Collagen III expressions significantly increased in both mRNA and protein levels at approximately 12J. Conclusions: There appears to be a threshold effect where there is very little increased collagen III mRNA and protein expression until the laser fluence reaches around 12J.

Original languageEnglish (US)
Pages (from-to)97-102
Number of pages6
JournalLasers in Surgery and Medicine
Issue number1
StatePublished - Jul 2005


  • Apligraf®
  • Collagen expression
  • Non-ablative laser

ASJC Scopus subject areas

  • Surgery


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