Abstract
Background and Objective: The 1,450-nm Smooth-beam™ Laser is a diode laser equipped with a cryogen cooling spray. Primary objectives were to evaluate the effects of this non-ablative laser on Apligraf® (bioengineered skin-substitute) and to document its use as a model for non-ablative procedures. We also measured the effects of laser fluence levels on collagen and elastin expression. Study Design/Materials and Methods: Three sheets of Apligraf® were used for this study. Each received six separate laser applications at 4J, 6J, 8J, 10J, 12J, and 14J. The sheets were then incubated with 10% CO 2 at 37°C and samples were collected and analyzed 3 days later, using RT-PCR and immunofluorescent staining. Results: Collagen III expressions significantly increased in both mRNA and protein levels at approximately 12J. Conclusions: There appears to be a threshold effect where there is very little increased collagen III mRNA and protein expression until the laser fluence reaches around 12J.
Original language | English (US) |
---|---|
Pages (from-to) | 97-102 |
Number of pages | 6 |
Journal | Lasers in Surgery and Medicine |
Volume | 37 |
Issue number | 1 |
DOIs | |
State | Published - Jul 2005 |
Keywords
- Apligraf®
- Collagen expression
- Non-ablative laser
ASJC Scopus subject areas
- Surgery