Effect of Th2 type cytokines on hCLCA1 and mucus expression in cystic fibrosis airways

Hans Peter Hauber, Francois Lavigne, Hsiao Ling Hung, Roy C Levitt, Qutayba Hamid

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Correlations between expression of interleukin (IL)-9, the calcium-activated chloride channel hCLCA1 and mucus expression in cystic fibrosis (CF) airways have suggested a causal relationship. To verify this hypothesis mucosal tissue from upper airways of CF patients (N=5) was stimulated with the Th2 type cytokines IL-4, IL-9, or IL-13. Expression of hCLCA1 mRNA and protein as well as mucus and mucin (MUC5AC) gene expression was quantified using real time PCR, immunohistochemistry (hCLCA1) and PAS staining (mucus). Th2 type cytokines significantly increased hCLCA1 protein expression (P<0.05) whereas increase in hCLCA1 mRNA expression failed to reach statistical significance (P>0.05). Mucin protein and MUC5AC mRNA expression were not significantly changed (P>0.05). These data suggest that Th2 type cytokines may increase hCLCA1 expression in CF but may not have a significant effect on mucus expression. Therefore the role of hCLCA1 as a mediator of mucus overexpression in CF has to be questioned.

Original languageEnglish
Pages (from-to)277-279
Number of pages3
JournalJournal of Cystic Fibrosis
Volume9
Issue number4
DOIs
StatePublished - Jul 1 2010
Externally publishedYes

Fingerprint

Mucus
Cystic Fibrosis
Cytokines
Interleukin-9
Mucins
Messenger RNA
Chloride Channels
Proteins
Interleukin-13
Interleukin-4
Real-Time Polymerase Chain Reaction
Mucous Membrane
Immunohistochemistry
Staining and Labeling
Gene Expression

Keywords

  • Airway
  • Cystic fibrosis
  • HCLCA1
  • Mucosa
  • Mucus
  • Th2 type cytokine

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Pediatrics, Perinatology, and Child Health

Cite this

Effect of Th2 type cytokines on hCLCA1 and mucus expression in cystic fibrosis airways. / Hauber, Hans Peter; Lavigne, Francois; Hung, Hsiao Ling; Levitt, Roy C; Hamid, Qutayba.

In: Journal of Cystic Fibrosis, Vol. 9, No. 4, 01.07.2010, p. 277-279.

Research output: Contribution to journalArticle

Hauber, Hans Peter ; Lavigne, Francois ; Hung, Hsiao Ling ; Levitt, Roy C ; Hamid, Qutayba. / Effect of Th2 type cytokines on hCLCA1 and mucus expression in cystic fibrosis airways. In: Journal of Cystic Fibrosis. 2010 ; Vol. 9, No. 4. pp. 277-279.
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