Abstract
In human lung adenocarcinoma cells exposed to vinblastine (VLB) and stained with fast green, large tubulin crystals were seen in the cytoplasm. In contrast, cells exposed to taxol had prominent bundles of stabilized microtubules. Pre- or co- incubation of cells with taxol before exposure to VLB prevented formation of the crystals. However, VLB did not prevent formation of stabilized microtubules by taxol if added at the same time. Under a fluorescent microscope VLB induced crystals stained with secondary antibodies against anti-α- and anti-β- tubulin antibodies. Neither of the drugs altered binding of the antibodies to these crystals.
| Original language | English |
|---|---|
| Pages (from-to) | 873-877 |
| Number of pages | 5 |
| Journal | Anticancer Research |
| Volume | 13 |
| Issue number | 4 |
| State | Published - Sep 3 1993 |
| Externally published | Yes |
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Keywords
- Crystallization
- Taxol
- Vinblastine
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Effect of taxol on vinblastine sulfate-induced crystallization of tubulin. / Ohta, S.; Krishan, A.; Nishio, K.; Ohmori, T.; Kunikane, H.; Inomata, M.; Takahashi, T.; Saijo, N.
In: Anticancer Research, Vol. 13, No. 4, 03.09.1993, p. 873-877.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of taxol on vinblastine sulfate-induced crystallization of tubulin
AU - Ohta, S.
AU - Krishan, A.
AU - Nishio, K.
AU - Ohmori, T.
AU - Kunikane, H.
AU - Inomata, M.
AU - Takahashi, T.
AU - Saijo, N.
PY - 1993/9/3
Y1 - 1993/9/3
N2 - In human lung adenocarcinoma cells exposed to vinblastine (VLB) and stained with fast green, large tubulin crystals were seen in the cytoplasm. In contrast, cells exposed to taxol had prominent bundles of stabilized microtubules. Pre- or co- incubation of cells with taxol before exposure to VLB prevented formation of the crystals. However, VLB did not prevent formation of stabilized microtubules by taxol if added at the same time. Under a fluorescent microscope VLB induced crystals stained with secondary antibodies against anti-α- and anti-β- tubulin antibodies. Neither of the drugs altered binding of the antibodies to these crystals.
AB - In human lung adenocarcinoma cells exposed to vinblastine (VLB) and stained with fast green, large tubulin crystals were seen in the cytoplasm. In contrast, cells exposed to taxol had prominent bundles of stabilized microtubules. Pre- or co- incubation of cells with taxol before exposure to VLB prevented formation of the crystals. However, VLB did not prevent formation of stabilized microtubules by taxol if added at the same time. Under a fluorescent microscope VLB induced crystals stained with secondary antibodies against anti-α- and anti-β- tubulin antibodies. Neither of the drugs altered binding of the antibodies to these crystals.
KW - Crystallization
KW - Taxol
KW - Vinblastine
UR - http://www.scopus.com/inward/record.url?scp=0027313525&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027313525&partnerID=8YFLogxK
M3 - Article
C2 - 7688938
AN - SCOPUS:0027313525
VL - 13
SP - 873
EP - 877
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 4
ER -