Effect of Surface-Attached Heparin on the Response of Potassium-Selective Electrodes

Kelly A. Brooks, John R. Allen, Pamela W. Feldhoff, Leonidas G Bachas

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Heparin (or hydrolyzed heparin) was covalently attached on the surface of derivatized cellulose triacetate membranes, which were subsequently impregnated with the potassium-selective ionophore valinomycin. The resulting ion-selective electrodes presented near-Nernstian response to potassium and had selectivity coefficients of the same order of magnitude as those of conventional poly(vinyl chloride)-based electrodes. It was found that the heparin layer does not alter significantly the response characteristics of the electrodes. The biological activity of the immobilized heparin (or hydrolyzed heparin) was measured in terms of its inactivation of blood coagulation factor Xa. It was found that the covalently anchored hydrolyzed heparin was not biologically active, but the immobilized heparin was able to inactivate factor Xa. Therefore, by covalently attaching heparin on the surface of ion-selective electrodes, electrodes with improved blood compatibility characteristics may be prepared.

Original languageEnglish (US)
Pages (from-to)1439-1443
Number of pages5
JournalAnalytical Chemistry
Volume68
Issue number8
StatePublished - Apr 15 1996
Externally publishedYes

Fingerprint

Heparin
Potassium
Electrodes
Vinyl Chloride
Valinomycin
Blood Coagulation Factors
Ionophores
Bioactivity
Blood
Membranes

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Effect of Surface-Attached Heparin on the Response of Potassium-Selective Electrodes. / Brooks, Kelly A.; Allen, John R.; Feldhoff, Pamela W.; Bachas, Leonidas G.

In: Analytical Chemistry, Vol. 68, No. 8, 15.04.1996, p. 1439-1443.

Research output: Contribution to journalArticle

Brooks, KA, Allen, JR, Feldhoff, PW & Bachas, LG 1996, 'Effect of Surface-Attached Heparin on the Response of Potassium-Selective Electrodes', Analytical Chemistry, vol. 68, no. 8, pp. 1439-1443.
Brooks, Kelly A. ; Allen, John R. ; Feldhoff, Pamela W. ; Bachas, Leonidas G. / Effect of Surface-Attached Heparin on the Response of Potassium-Selective Electrodes. In: Analytical Chemistry. 1996 ; Vol. 68, No. 8. pp. 1439-1443.
@article{bbbcf400c73040d5a64c4749f8b30f61,
title = "Effect of Surface-Attached Heparin on the Response of Potassium-Selective Electrodes",
abstract = "Heparin (or hydrolyzed heparin) was covalently attached on the surface of derivatized cellulose triacetate membranes, which were subsequently impregnated with the potassium-selective ionophore valinomycin. The resulting ion-selective electrodes presented near-Nernstian response to potassium and had selectivity coefficients of the same order of magnitude as those of conventional poly(vinyl chloride)-based electrodes. It was found that the heparin layer does not alter significantly the response characteristics of the electrodes. The biological activity of the immobilized heparin (or hydrolyzed heparin) was measured in terms of its inactivation of blood coagulation factor Xa. It was found that the covalently anchored hydrolyzed heparin was not biologically active, but the immobilized heparin was able to inactivate factor Xa. Therefore, by covalently attaching heparin on the surface of ion-selective electrodes, electrodes with improved blood compatibility characteristics may be prepared.",
author = "Brooks, {Kelly A.} and Allen, {John R.} and Feldhoff, {Pamela W.} and Bachas, {Leonidas G}",
year = "1996",
month = "4",
day = "15",
language = "English (US)",
volume = "68",
pages = "1439--1443",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "8",

}

TY - JOUR

T1 - Effect of Surface-Attached Heparin on the Response of Potassium-Selective Electrodes

AU - Brooks, Kelly A.

AU - Allen, John R.

AU - Feldhoff, Pamela W.

AU - Bachas, Leonidas G

PY - 1996/4/15

Y1 - 1996/4/15

N2 - Heparin (or hydrolyzed heparin) was covalently attached on the surface of derivatized cellulose triacetate membranes, which were subsequently impregnated with the potassium-selective ionophore valinomycin. The resulting ion-selective electrodes presented near-Nernstian response to potassium and had selectivity coefficients of the same order of magnitude as those of conventional poly(vinyl chloride)-based electrodes. It was found that the heparin layer does not alter significantly the response characteristics of the electrodes. The biological activity of the immobilized heparin (or hydrolyzed heparin) was measured in terms of its inactivation of blood coagulation factor Xa. It was found that the covalently anchored hydrolyzed heparin was not biologically active, but the immobilized heparin was able to inactivate factor Xa. Therefore, by covalently attaching heparin on the surface of ion-selective electrodes, electrodes with improved blood compatibility characteristics may be prepared.

AB - Heparin (or hydrolyzed heparin) was covalently attached on the surface of derivatized cellulose triacetate membranes, which were subsequently impregnated with the potassium-selective ionophore valinomycin. The resulting ion-selective electrodes presented near-Nernstian response to potassium and had selectivity coefficients of the same order of magnitude as those of conventional poly(vinyl chloride)-based electrodes. It was found that the heparin layer does not alter significantly the response characteristics of the electrodes. The biological activity of the immobilized heparin (or hydrolyzed heparin) was measured in terms of its inactivation of blood coagulation factor Xa. It was found that the covalently anchored hydrolyzed heparin was not biologically active, but the immobilized heparin was able to inactivate factor Xa. Therefore, by covalently attaching heparin on the surface of ion-selective electrodes, electrodes with improved blood compatibility characteristics may be prepared.

UR - http://www.scopus.com/inward/record.url?scp=0030121864&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030121864&partnerID=8YFLogxK

M3 - Article

C2 - 8651503

AN - SCOPUS:0030121864

VL - 68

SP - 1439

EP - 1443

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 8

ER -