Effect of Storage on Sheep Erythrocytes Used in Complement Studies

B. J. Fogel, C. E. Shields, W. A. Hook, E. H. Fife

Research output: Contribution to journalArticlepeer-review


Sheep erythrocytes stored for periods up to 4 weeks in modified Alsever's solution exhibited significant and progressive increases in susceptibility to immune hemolysis in assays conducted with a constant source of human C′. Moreover, the increased susceptibility to immune hemolysis closely paralleled the changes of osmotic fragility and a tendency to undergo spontaneous lysis. The findings suggested an intrinsic relationship between immune hemolysis and osmotic fragility. It was noted that cells stored longer than 3 weeks in modified Alsever's solution could be unduly fragile and hypersensitive to immune lysis thus rendering them unsuitable for serologic tests.

Original languageEnglish (US)
Pages (from-to)186-189
Number of pages4
JournalProceedings of the Society for Experimental Biology and Medicine
Issue number1
StatePublished - Jan 1966

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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