Effect of sodium cyanate upon the function of normal human polymorphonuclear leukocytes

Kenneth R. Ratzan, Constancio Giraudo, Ceferina Amado, Isabel Lauredo, Glen Horowitz

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Sodium cyanate, a drug that prevents sickling of hemoglobin S by virtue of its irreversible carbamylation of the N terminal amino group of valine, was studied for its effect upon the function of normal human polymorphonuclear leukocytes. In concentrations of 500 and 100 μg/ml, sodium cyanate was found to inhibit killing by neutrophils of Staphylococcus epidermidis and Escherichia coli but not of Streptococcus faecalis. Viability of cells and phagocytosis were not affected by cyanate; however, production of [ 14C]carbon dioxide from [1 14C]glucose and the iodination of 125I during phagocytosis were significantly impaired. Cyanate is thought to inhibit the bactericidal activity of neutrophils by interfering with the oxidative metabolism of glucose via the hexose monophosphate shunt (thereby decreasing production of H 2O 2) and by inhibiting iodination of ingested bacteria (either by competing with iodide as the oxidizable cofactor or by inhibiting myeloperoxidase). Since these effects of cyanate were all reversible by washing the neutrophils free of the drug, it is unlikely that they are due to amino carbamylation.

Original languageEnglish (US)
Pages (from-to)73-80
Number of pages8
JournalUnknown Journal
Volume131
Issue numbersupmay
DOIs
StatePublished - 1975

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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