Abstract
The potentiometric response of several polymer membrane based ion‐selective electrodes prepared with hydrophobic vitamin B12 derivatives is described. The effect of different proteins on the properties of these electrodes is discussed. It is suggested that the observed changes in potential upon addition of proteins are due mainly to nonspecific interactions between the proteins and the polymer membrane. Reduction of the nonspecific binding effect is possible by using a polymer matrix that contains free hydroxyl groups. The observed results agree with a theoretical model, which predicts that the nonspecific binding effect is more evident when membranes loaded with relatively small amounts of ionophore are used. The implications of these observations for the development of biosensors are discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 177-182 |
| Number of pages | 6 |
| Journal | Electroanalysis |
| Volume | 3 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
- Electrochemistry
Cite this
Effect of proteins on the response of anion‐selective electrodes based on vitamin B12 derivatives. / Florido, Antonio; Daunert, Sylvia; Bachas, Leonidas G.
In: Electroanalysis, Vol. 3, No. 3, 1991, p. 177-182.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of proteins on the response of anion‐selective electrodes based on vitamin B12 derivatives
AU - Florido, Antonio
AU - Daunert, Sylvia
AU - Bachas, Leonidas G
PY - 1991
Y1 - 1991
N2 - The potentiometric response of several polymer membrane based ion‐selective electrodes prepared with hydrophobic vitamin B12 derivatives is described. The effect of different proteins on the properties of these electrodes is discussed. It is suggested that the observed changes in potential upon addition of proteins are due mainly to nonspecific interactions between the proteins and the polymer membrane. Reduction of the nonspecific binding effect is possible by using a polymer matrix that contains free hydroxyl groups. The observed results agree with a theoretical model, which predicts that the nonspecific binding effect is more evident when membranes loaded with relatively small amounts of ionophore are used. The implications of these observations for the development of biosensors are discussed.
AB - The potentiometric response of several polymer membrane based ion‐selective electrodes prepared with hydrophobic vitamin B12 derivatives is described. The effect of different proteins on the properties of these electrodes is discussed. It is suggested that the observed changes in potential upon addition of proteins are due mainly to nonspecific interactions between the proteins and the polymer membrane. Reduction of the nonspecific binding effect is possible by using a polymer matrix that contains free hydroxyl groups. The observed results agree with a theoretical model, which predicts that the nonspecific binding effect is more evident when membranes loaded with relatively small amounts of ionophore are used. The implications of these observations for the development of biosensors are discussed.
UR - http://www.scopus.com/inward/record.url?scp=84987592533&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84987592533&partnerID=8YFLogxK
U2 - 10.1002/elan.1140030307
DO - 10.1002/elan.1140030307
M3 - Article
AN - SCOPUS:84987592533
VL - 3
SP - 177
EP - 182
JO - Electroanalysis
JF - Electroanalysis
SN - 1040-0397
IS - 3
ER -