Effect of protein tyrosine phosphatase non-receptor type 12 (PTPN12) downregulation on the radiosensitivity of non-small cell lung cancer

Wenxia Hu, Zhikun Liu, Weili Zhang, Scott Welford, Jingwei Su

Research output: Contribution to journalArticle

Abstract

Objective: To identify the relationship between the expression of protein tyrosine phosphatase non-receptor type 12 (PTPN12) and radiotherapy effect in non-small cell lung cancer (NSCLC) tissues and to determine whether PTPN12 deficiency can sensitize lung cancer cells to irradiation. Methods: From September 2013 to October 2014, 92 NSCLC patients undergoing radiotherapy with or without platinum-based combination chemotherapy were analyzed retrospectively. Before the treatment, PTPN12 expression was detected through immunohistochemistry. After the completion of radiotherapy, the patients' responses were assessed and radiotherapeutic efficacy analyzed. The human NSCLC cell line H1299 was infected with shPTPN12 knockdown, and colony survival assay was analyzed after irradiation. Chi-square test was used to examine the correlation between PTPN12 expression and clinicopathological characteristics. Univariate analyses and Logistic regression test were used to analyze the relationship between clinicopathological characteristics and radiotherapeutic response. Results: Patients with low PTPN12 expression were more sensitive to radiotherapy than those with high PTPN12 expression (80.0% vs. 57.1%, P=0.018). Multivariate analysis showed that PTPN12 expression was the only independent predictor of radiotherapeutic response in NSCLC. The H1299-shPTPN12-knockdown cells were sensitive to irradiation.Conclusions: The results of the study indicated that downregulation of PTPN12 improved the radiosensitivity of NSCLC cells.

Original languageEnglish (US)
Pages (from-to)285-290
Number of pages6
JournalChinese Journal of Clinical Oncology
Volume43
Issue number7
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

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Non-Receptor Type 12 Protein Tyrosine Phosphatase
Radiation Tolerance
Non-Small Cell Lung Carcinoma
Down-Regulation
Radiotherapy
Chi-Square Distribution
Combination Drug Therapy
Platinum
Lung Neoplasms
Multivariate Analysis
Logistic Models
Immunohistochemistry

Keywords

  • H1299 cellline
  • Non-small cell lung cancer
  • PTPN12
  • Radiosensitivity
  • Radiotherapeutic efficacy

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Effect of protein tyrosine phosphatase non-receptor type 12 (PTPN12) downregulation on the radiosensitivity of non-small cell lung cancer. / Hu, Wenxia; Liu, Zhikun; Zhang, Weili; Welford, Scott; Su, Jingwei.

In: Chinese Journal of Clinical Oncology, Vol. 43, No. 7, 01.01.2016, p. 285-290.

Research output: Contribution to journalArticle

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AU - Su, Jingwei

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AB - Objective: To identify the relationship between the expression of protein tyrosine phosphatase non-receptor type 12 (PTPN12) and radiotherapy effect in non-small cell lung cancer (NSCLC) tissues and to determine whether PTPN12 deficiency can sensitize lung cancer cells to irradiation. Methods: From September 2013 to October 2014, 92 NSCLC patients undergoing radiotherapy with or without platinum-based combination chemotherapy were analyzed retrospectively. Before the treatment, PTPN12 expression was detected through immunohistochemistry. After the completion of radiotherapy, the patients' responses were assessed and radiotherapeutic efficacy analyzed. The human NSCLC cell line H1299 was infected with shPTPN12 knockdown, and colony survival assay was analyzed after irradiation. Chi-square test was used to examine the correlation between PTPN12 expression and clinicopathological characteristics. Univariate analyses and Logistic regression test were used to analyze the relationship between clinicopathological characteristics and radiotherapeutic response. Results: Patients with low PTPN12 expression were more sensitive to radiotherapy than those with high PTPN12 expression (80.0% vs. 57.1%, P=0.018). Multivariate analysis showed that PTPN12 expression was the only independent predictor of radiotherapeutic response in NSCLC. The H1299-shPTPN12-knockdown cells were sensitive to irradiation.Conclusions: The results of the study indicated that downregulation of PTPN12 improved the radiosensitivity of NSCLC cells.

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