Effect of pretreatment under various cationic conditions on acetylcholine content and choline transport in rat whole brain synaptosomes

M. H. Weiler, Richard S Jope, D. J. Jenden

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Abstract

The effects of different ionic environments were measured on the concentration of acetylcholine (ACh) from synaptosomes and their effect on subsequent high affinity choline (Ch) transport and ACh synthesis after resuspension of the synaptosomes in the normal Krebs medium. KCl (40mM) was used to induce ACh release and reduce synaptosomal ACh content. The effects of Na+ omission, Ca2+ omission, and high Mg2+ on spontaneous (KCl: 4.75 mM) and potassium induced (KCl:40 mM) ACh release and other cholinergic parameters are presented. The high affinity transport of Ch was more highly correlated with the reciprocal of the ACh level (r=0.934, P=9.7 x 10-4) than with the ACh release rate during preincubation (r=0.792, P=3.4 x 10-2). The results are consistent with the view that the consequences of the various ionic conditions on Ch transport and ACh synthesis are dependent on their effects on intrasynaptosomal ACh levels and only secondarily on synaptosomal ACh release.

Original languageEnglish
Pages (from-to)789-796
Number of pages8
JournalJournal of Neurochemistry
Volume31
Issue number4
StatePublished - Dec 1 1978
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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