Effect of platelet-activating factor on the process of cellular differentiation of Herpetomonas muscarum muscarum

Angela H.C.S. Lopes, Patricia M.L. Dutra, Claudia O. Rodrigues, Maurilio J. Soares, Jayme Angluster, Renato S.B. Cordeiro

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

The effects of platelet-activating factor (PAF), at doses ranging from 10-6 M to 10-10 M, on cell growth and on cell differentiation of Herpetomonas muscarum were investigated. Cell differentiation was evaluated by both light and electron microscopy. At the concentrations used, PAF did not interfere with the protozoan growth. However, parasites grown in the presence of PAF (10-6 M) were significantly more differentiated than those grown in the absence of PAF, since the first day of culture. On the first two days of culture, PAF doses ranging from 10-10 M to 10-7 M, did not significantly interfere with the differentiation of these parasites, although after the third day of culture, all PAF doses used significantly increased the protozoan differentiation. Specific PAF receptor antagonists totally abrogated (WEB 2086 and WEB 2170) or significantly decreased (BN 52021) PAF effect on cell differentiation. These findings indicate PAF triggers the process of cell differentiation in Herpetomonas muscarum and suggest these parasites have rcceptors for PAF.

Original languageEnglish (US)
Pages (from-to)321-325
Number of pages5
JournalJournal of Eukaryotic Microbiology
Volume44
Issue number4
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

Keywords

  • Cell receptors
  • PAF receptor antagonists
  • Trypanosomatids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Agricultural and Biological Sciences (miscellaneous)
  • Microbiology

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