Effect of platelet-activating factor on cell differentiation of Trypanosoma cruzi

Claudia O. Rodrigues; Patrícia M.L. Dutra; Thais Souto-Padrón; Renato S.B. Cordeiro; Angela H.C.S. Lopes

doi:10.1006/bbrc.1996.0965

Effect of platelet-activating factor on cell differentiation of Trypanosoma cruzi

Claudia O. Rodrigues, Patrícia M.L. Dutra, Thais Souto-Padrón, Renato S.B. Cordeiro, Angela H.C.S. Lopes

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The effects of platelet-activating factor (PAF), at 10^-6 M and 10^-9 M, on cell growth and cell differentiation of Trypanosoma cruzi were investigated. Cell differentiation was evaluated by both light and electron microscopy. At the concentrations used, PAF slightly interfered with the protozoan growth. However, parasites growth in the presence of PAF were significantly more differentiated than those grown in the absence of PAF, beginning on the fourth day of culture. A specific PAF receptor antagonist (WEB 2086) totally abrogated PAF effect on cell differentiation. These findings indicate that PAF triggers the process of cell differentiation in T. cruzi and suggest that these parasites have receptors for PAF.

Original language	English (US)
Pages (from-to)	735-740
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	223
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1996.0965
State	Published - Jun 25 1996
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Effect of platelet-activating factor on cell differentiation of Trypanosoma cruzi",

abstract = "The effects of platelet-activating factor (PAF), at 10-6 M and 10-9 M, on cell growth and cell differentiation of Trypanosoma cruzi were investigated. Cell differentiation was evaluated by both light and electron microscopy. At the concentrations used, PAF slightly interfered with the protozoan growth. However, parasites growth in the presence of PAF were significantly more differentiated than those grown in the absence of PAF, beginning on the fourth day of culture. A specific PAF receptor antagonist (WEB 2086) totally abrogated PAF effect on cell differentiation. These findings indicate that PAF triggers the process of cell differentiation in T. cruzi and suggest that these parasites have receptors for PAF.",

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AU - Rodrigues, Claudia O.

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AU - Cordeiro, Renato S.B.

AU - Lopes, Angela H.C.S.

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