TY - JOUR
T1 - Effect of platelet-activating factor on cell differentiation of Trypanosoma cruzi
AU - Rodrigues, Claudia O.
AU - Dutra, Patrícia M.L.
AU - Souto-Padrón, Thais
AU - Cordeiro, Renato S.B.
AU - Lopes, Angela H.C.S.
N1 - Funding Information:
1This work was supported by FINEP, CNPq, CEPG-UFRJ and CAPES 2To whom correspondence should be addressed. Fax #270-8793; email:immgahc@microbio.ufrj.br Abbreviations: PAF, platelet-activating factor; BHI, brain heart infusion; TAUP, triatomine artificial
Funding Information:
We thank Dr. Fernando P. Câmara for the statistical analysis, Dr. José M. C. Ribeiro for revising this manuscript, Dr. Jayme Angluster for helpful discussions, and Mr. Luciano A. M. Grillo for technical assistance. This research was supported by FINEP, CNPq, CAPES, and CEPG-UFRJ.
PY - 1996/6/25
Y1 - 1996/6/25
N2 - The effects of platelet-activating factor (PAF), at 10-6 M and 10-9 M, on cell growth and cell differentiation of Trypanosoma cruzi were investigated. Cell differentiation was evaluated by both light and electron microscopy. At the concentrations used, PAF slightly interfered with the protozoan growth. However, parasites growth in the presence of PAF were significantly more differentiated than those grown in the absence of PAF, beginning on the fourth day of culture. A specific PAF receptor antagonist (WEB 2086) totally abrogated PAF effect on cell differentiation. These findings indicate that PAF triggers the process of cell differentiation in T. cruzi and suggest that these parasites have receptors for PAF.
AB - The effects of platelet-activating factor (PAF), at 10-6 M and 10-9 M, on cell growth and cell differentiation of Trypanosoma cruzi were investigated. Cell differentiation was evaluated by both light and electron microscopy. At the concentrations used, PAF slightly interfered with the protozoan growth. However, parasites growth in the presence of PAF were significantly more differentiated than those grown in the absence of PAF, beginning on the fourth day of culture. A specific PAF receptor antagonist (WEB 2086) totally abrogated PAF effect on cell differentiation. These findings indicate that PAF triggers the process of cell differentiation in T. cruzi and suggest that these parasites have receptors for PAF.
UR - http://www.scopus.com/inward/record.url?scp=0030601084&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030601084&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.0965
DO - 10.1006/bbrc.1996.0965
M3 - Article
C2 - 8687466
AN - SCOPUS:0030601084
VL - 223
SP - 735
EP - 740
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -