Effect of pesticides on microbial communities in container aquatic habitats

Ephantus J. Muturi, Ravi Kiran Donthu, Christopher J. Fields, Imelda Moise, Chang Hyun Kim

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Container aquatic habitats support a specialized community of macroinvertebrates (e.g. mosquitoes) that feed on microbial communities associated with decaying organic matter. These aquatic habitats are often embedded within and around agricultural lands and are frequently exposed to pesticides. We used a microcosm approach to examine the single and combined effects of two herbicides (atrazine, glyphosate), and three insecticides (malathion, carbaryl, permethrin) on microbial communities of container aquatic habitats. MiSeq sequencing of the V4 region of both bacterial and archaeal 16S rRNA gene was used to characterize the microbial communities of indoor microcosms that were either exposed to each pesticide alone, a mix of herbicides, a mix of insecticides, or a mix of all five insecticides. Individual insecticides but not herbicides reduced the microbial diversity and richness and two insecticides, carbaryl and permethrin, also altered the microbial community structure. A mixture of herbicides had no effect on microbial diversity or structure but a mixture of insecticides or all five pesticides reduced microbial diversity and altered the community structure. These findings suggest that exposure of aquatic ecosystems to individual pesticides or their mixtures can disrupt aquatic microbial communities and there is need to decipher how these changes affect resident macroinvertebrate communities.

Original languageEnglish (US)
Article number44565
JournalScientific Reports
Volume7
DOIs
StatePublished - Mar 16 2017

Fingerprint

aquatic habitat
microbial communities
containers
insecticides
pesticides
herbicides
carbaryl
permethrin
macroinvertebrates
community structure
malathion
atrazine
glyphosate
agricultural land
Culicidae
ribosomal RNA
organic matter
genes

ASJC Scopus subject areas

  • General

Cite this

Effect of pesticides on microbial communities in container aquatic habitats. / Muturi, Ephantus J.; Donthu, Ravi Kiran; Fields, Christopher J.; Moise, Imelda; Kim, Chang Hyun.

In: Scientific Reports, Vol. 7, 44565, 16.03.2017.

Research output: Contribution to journalArticle

Muturi, Ephantus J. ; Donthu, Ravi Kiran ; Fields, Christopher J. ; Moise, Imelda ; Kim, Chang Hyun. / Effect of pesticides on microbial communities in container aquatic habitats. In: Scientific Reports. 2017 ; Vol. 7.
@article{3051488aca6c45f7bf751604614e60ba,
title = "Effect of pesticides on microbial communities in container aquatic habitats",
abstract = "Container aquatic habitats support a specialized community of macroinvertebrates (e.g. mosquitoes) that feed on microbial communities associated with decaying organic matter. These aquatic habitats are often embedded within and around agricultural lands and are frequently exposed to pesticides. We used a microcosm approach to examine the single and combined effects of two herbicides (atrazine, glyphosate), and three insecticides (malathion, carbaryl, permethrin) on microbial communities of container aquatic habitats. MiSeq sequencing of the V4 region of both bacterial and archaeal 16S rRNA gene was used to characterize the microbial communities of indoor microcosms that were either exposed to each pesticide alone, a mix of herbicides, a mix of insecticides, or a mix of all five insecticides. Individual insecticides but not herbicides reduced the microbial diversity and richness and two insecticides, carbaryl and permethrin, also altered the microbial community structure. A mixture of herbicides had no effect on microbial diversity or structure but a mixture of insecticides or all five pesticides reduced microbial diversity and altered the community structure. These findings suggest that exposure of aquatic ecosystems to individual pesticides or their mixtures can disrupt aquatic microbial communities and there is need to decipher how these changes affect resident macroinvertebrate communities.",
author = "Muturi, {Ephantus J.} and Donthu, {Ravi Kiran} and Fields, {Christopher J.} and Imelda Moise and Kim, {Chang Hyun}",
year = "2017",
month = "3",
day = "16",
doi = "10.1038/srep44565",
language = "English (US)",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Effect of pesticides on microbial communities in container aquatic habitats

AU - Muturi, Ephantus J.

AU - Donthu, Ravi Kiran

AU - Fields, Christopher J.

AU - Moise, Imelda

AU - Kim, Chang Hyun

PY - 2017/3/16

Y1 - 2017/3/16

N2 - Container aquatic habitats support a specialized community of macroinvertebrates (e.g. mosquitoes) that feed on microbial communities associated with decaying organic matter. These aquatic habitats are often embedded within and around agricultural lands and are frequently exposed to pesticides. We used a microcosm approach to examine the single and combined effects of two herbicides (atrazine, glyphosate), and three insecticides (malathion, carbaryl, permethrin) on microbial communities of container aquatic habitats. MiSeq sequencing of the V4 region of both bacterial and archaeal 16S rRNA gene was used to characterize the microbial communities of indoor microcosms that were either exposed to each pesticide alone, a mix of herbicides, a mix of insecticides, or a mix of all five insecticides. Individual insecticides but not herbicides reduced the microbial diversity and richness and two insecticides, carbaryl and permethrin, also altered the microbial community structure. A mixture of herbicides had no effect on microbial diversity or structure but a mixture of insecticides or all five pesticides reduced microbial diversity and altered the community structure. These findings suggest that exposure of aquatic ecosystems to individual pesticides or their mixtures can disrupt aquatic microbial communities and there is need to decipher how these changes affect resident macroinvertebrate communities.

AB - Container aquatic habitats support a specialized community of macroinvertebrates (e.g. mosquitoes) that feed on microbial communities associated with decaying organic matter. These aquatic habitats are often embedded within and around agricultural lands and are frequently exposed to pesticides. We used a microcosm approach to examine the single and combined effects of two herbicides (atrazine, glyphosate), and three insecticides (malathion, carbaryl, permethrin) on microbial communities of container aquatic habitats. MiSeq sequencing of the V4 region of both bacterial and archaeal 16S rRNA gene was used to characterize the microbial communities of indoor microcosms that were either exposed to each pesticide alone, a mix of herbicides, a mix of insecticides, or a mix of all five insecticides. Individual insecticides but not herbicides reduced the microbial diversity and richness and two insecticides, carbaryl and permethrin, also altered the microbial community structure. A mixture of herbicides had no effect on microbial diversity or structure but a mixture of insecticides or all five pesticides reduced microbial diversity and altered the community structure. These findings suggest that exposure of aquatic ecosystems to individual pesticides or their mixtures can disrupt aquatic microbial communities and there is need to decipher how these changes affect resident macroinvertebrate communities.

UR - http://www.scopus.com/inward/record.url?scp=85015646728&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85015646728&partnerID=8YFLogxK

U2 - 10.1038/srep44565

DO - 10.1038/srep44565

M3 - Article

C2 - 28300212

AN - SCOPUS:85015646728

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 44565

ER -