Effect of PCBs on mouse lung tumorigenesis induced by 1-nitropyrene: A preliminary report

Y. Nakanishi, F. Bai, K. Takayama, X. H. Pei, K. Inoue, S. I. Osaki, M. Izumi, Y. Takaki, N. Hara, H. Tokiwa, Y. Masuda

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4 Scopus citations

Abstract

We have analyzed the effect of polychlorinated biphenyls (PCB, Kanechlor 400) on 1-nitropyrene (1-NP) induced lung tumor. Male A/J mice (6 weeks old) were used for the experiment. A total of 2.5 mg/kg PCB was administered intraperitoneally (PCB group), a total of 0.38 mmol/kg 1-NP was administered intraperitoneally for 17 times (1-NP group), PCB was administered followed by ip injection of 1-NP (PCB + 1-NP group), and only vehicle was administered (control group). The lung lesions induced were examined 18 weeks after the final treatment with 1-NP or vehicle. In control group, no neoplastic lesion in the lung was induced. In PCB group, only one lesion with adenoma was induced. In 1-NP group, various kinds of lung neoplastic lesions including hyperplasia, adenoma and adenocarcinoma were induced. In PCB + 1-NP group, both the number and size of tumors induced were significantly more than those in 1-NP group. In addition, the number of adenocarcinoma formed was more in PCB + 1-NP group than in 1-NP group. Each lesion was microdissected to collect and analyze DNA of the targeted tissue. K-ras gene mutation was detected in part of adenoma lesions and all the carcinoma lesions. The mutation was found in either 1-NP or PCB + 1-NP group, but not in control and PCB group. The pattern of K-ras mutation was CAA to CGA in codon 61 or GGT to GAT in codon 12. There was no difference in the pattern of K-ras mutation despite of the pretreatment with PCB. Although the present data are from small sample size, it was suggested that PCB may promote (but not initiate) 1-NP induced lung tumorigenesis, and may not induce K-ras mutation directly in the experimental system.

Original languageEnglish (US)
Pages (from-to)231-237
Number of pages7
JournalFukuoka Acta Medica
Volume90
Issue number5
StatePublished - Sep 3 1999
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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    Nakanishi, Y., Bai, F., Takayama, K., Pei, X. H., Inoue, K., Osaki, S. I., Izumi, M., Takaki, Y., Hara, N., Tokiwa, H., & Masuda, Y. (1999). Effect of PCBs on mouse lung tumorigenesis induced by 1-nitropyrene: A preliminary report. Fukuoka Acta Medica, 90(5), 231-237.