Effect of ozone on the postnatal development of lamb mucociliary apparatus

A. T. Mariassy, W. M. Abraham, R. J. Phipps, M. W. Sielczak, Adam Wanner

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We determined whether exposure to O3 early in the postnatal period impairs the normal development of the mucociliary apparatus in lambs and whether such changes lead to prolonged abnormalities in mucociliary function. Lambs were exposed to air (controls) or to 1 ppm O3 for 4 h/day for 5 days during the 1st wk of life. Tracheal mucus velocity (TMV), a marker of lung mucociliary clearance, was measured in vivo at birth (0 wk) and up to 24 wk later, and tracheal secretory function was measured (in vitro) and the morphology of the tracheal mucosa was determined at 0 and 2 wk in both groups. In the control group, TMV increased 94% from 0 to 2 wk (P < 0.05), continued to increase until reaching a plateau at 8 wk, and then remained constant from 8 to 24 wk. In contrast, O3-exposed lambs showed a 24% decrease in TMV from 0 to 2 wk (P < 0.05 vs. control), and throughout the remaining time TMV remained below (P < 0.05) that observed in control lambs. O3 exposure partially prevented the age-dependent decrease in basal secretion of tracheal macromolecules normally observed between 0 and 2 wk. These changes in secretory function were associated with a significant increase in tissue conductance (37%, P < 0.05 vs. 0 wk), predominantly the results of active chloride secretion. The functional changes induced by O3 were associated with a retardation of the normal morphological development of the tracheal epithelium. In control lambs mucus cell populations declined from 21% at birth to 16% at 2 wk (P < 0.05) and ciliated cell populations rose from 34 to 38% (P < 0.05). In the O3-exposed lambs, however, mucus and ciliated cell percentages remained at newborn levels (22 and 33%, respectively, both P < 0.05 vs. control). These findings indicate that O3 exposure during the 1st wk of life retards the normal development of the mucociliary system in sheep and that such changes may lead to prolonged impairment of lung mucociliary clearance.

Original languageEnglish
Pages (from-to)2504-2510
Number of pages7
JournalJournal of Applied Physiology
Volume68
Issue number6
StatePublished - Jan 1 1990

Fingerprint

Ozone
Mucus
Mucociliary Clearance
Parturition
Lung
Population
Chlorides
Sheep
Mucous Membrane
Epithelium
Air
Control Groups

Keywords

  • morphology
  • mucus secretion
  • newborn
  • pollutant
  • tracheal mucus velocity

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Mariassy, A. T., Abraham, W. M., Phipps, R. J., Sielczak, M. W., & Wanner, A. (1990). Effect of ozone on the postnatal development of lamb mucociliary apparatus. Journal of Applied Physiology, 68(6), 2504-2510.

Effect of ozone on the postnatal development of lamb mucociliary apparatus. / Mariassy, A. T.; Abraham, W. M.; Phipps, R. J.; Sielczak, M. W.; Wanner, Adam.

In: Journal of Applied Physiology, Vol. 68, No. 6, 01.01.1990, p. 2504-2510.

Research output: Contribution to journalArticle

Mariassy, AT, Abraham, WM, Phipps, RJ, Sielczak, MW & Wanner, A 1990, 'Effect of ozone on the postnatal development of lamb mucociliary apparatus', Journal of Applied Physiology, vol. 68, no. 6, pp. 2504-2510.
Mariassy AT, Abraham WM, Phipps RJ, Sielczak MW, Wanner A. Effect of ozone on the postnatal development of lamb mucociliary apparatus. Journal of Applied Physiology. 1990 Jan 1;68(6):2504-2510.
Mariassy, A. T. ; Abraham, W. M. ; Phipps, R. J. ; Sielczak, M. W. ; Wanner, Adam. / Effect of ozone on the postnatal development of lamb mucociliary apparatus. In: Journal of Applied Physiology. 1990 ; Vol. 68, No. 6. pp. 2504-2510.
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abstract = "We determined whether exposure to O3 early in the postnatal period impairs the normal development of the mucociliary apparatus in lambs and whether such changes lead to prolonged abnormalities in mucociliary function. Lambs were exposed to air (controls) or to 1 ppm O3 for 4 h/day for 5 days during the 1st wk of life. Tracheal mucus velocity (TMV), a marker of lung mucociliary clearance, was measured in vivo at birth (0 wk) and up to 24 wk later, and tracheal secretory function was measured (in vitro) and the morphology of the tracheal mucosa was determined at 0 and 2 wk in both groups. In the control group, TMV increased 94{\%} from 0 to 2 wk (P < 0.05), continued to increase until reaching a plateau at 8 wk, and then remained constant from 8 to 24 wk. In contrast, O3-exposed lambs showed a 24{\%} decrease in TMV from 0 to 2 wk (P < 0.05 vs. control), and throughout the remaining time TMV remained below (P < 0.05) that observed in control lambs. O3 exposure partially prevented the age-dependent decrease in basal secretion of tracheal macromolecules normally observed between 0 and 2 wk. These changes in secretory function were associated with a significant increase in tissue conductance (37{\%}, P < 0.05 vs. 0 wk), predominantly the results of active chloride secretion. The functional changes induced by O3 were associated with a retardation of the normal morphological development of the tracheal epithelium. In control lambs mucus cell populations declined from 21{\%} at birth to 16{\%} at 2 wk (P < 0.05) and ciliated cell populations rose from 34 to 38{\%} (P < 0.05). In the O3-exposed lambs, however, mucus and ciliated cell percentages remained at newborn levels (22 and 33{\%}, respectively, both P < 0.05 vs. control). These findings indicate that O3 exposure during the 1st wk of life retards the normal development of the mucociliary system in sheep and that such changes may lead to prolonged impairment of lung mucociliary clearance.",
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