Effect of oxygen concentration on the growth and drug sensitivity of human melanoma cells in soft-agar clonogenic assay

V. Gupta, A. Krishan

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Tumor cells from a human melanoma xenograft show better colony growth and plating efficiency in soft agar when incubated in 5% O2 atmosphere than at a higher concentration of 20% O2. Exposure to low drug concentrations under a 20% (but not 5%) O2 atmosphere stimulated plating efficiency. Enhanced sensitivity (4- to 20-fold) to a variety of chemotherapeutic agents was seen in cells incubated under low but not the higher O2 concentration. These studies suggest the important role of O2 concentration in tumor colony growth, plating efficiency, and drug cytotoxicity for human solid tumors in vitro.

Original languageEnglish
Pages (from-to)1005-1007
Number of pages3
JournalCancer Research
Volume42
Issue number3
StatePublished - Jan 1 1982
Externally publishedYes

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Agar
Melanoma
Oxygen
Atmosphere
Growth
Pharmaceutical Preparations
Neoplasms
Heterografts
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Effect of oxygen concentration on the growth and drug sensitivity of human melanoma cells in soft-agar clonogenic assay. / Gupta, V.; Krishan, A.

In: Cancer Research, Vol. 42, No. 3, 01.01.1982, p. 1005-1007.

Research output: Contribution to journalArticle

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