Effect of osmolytes on the binding of EGR1 transcription factor to DNA

David C. Mikles, Vikas Bhat, Brett J. Schuchardt, Caleb B. McDonald, Amjad Farooq

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Osmolytes play a key role in maintaining protein stability and mediating macromolecular interactions within the intracellular environment of the cell. Herein, we show that osmolytes such as glycerol, sucrose, and polyethylene glycol 400 (PEG400) mitigate the binding of early growth response (protein) 1 (EGR1) transcription factor to DNA in a differential manner. Thus, while physiological concentrations of glycerol only moderately reduce the binding affinity, addition of sucrose and PEG400 is concomitant with a loss in the binding affinity by an order of magnitude. This salient observation suggests that EGR1 is most likely subject to conformational equilibrium and that the osmolytes exert their effect via favorable interactions with the unliganded conformation. Consistent with this notion, our analysis reveals that while EGR1 displays rather high structural stability in complex with DNA, the unliganded conformation becomes significantly destabilized in solution. In particular, while liganded EGR1 adopts a well-defined arc-like architecture, the unliganded protein samples a comparatively large conformational space between two distinct states that periodically interconvert between an elongated rod-like shape and an arc-like conformation on a submicrosecond time scale.

Original languageEnglish (US)
Pages (from-to)74-87
Number of pages14
JournalBiopolymers
Volume103
Issue number2
DOIs
StatePublished - Feb 1 2015

Fingerprint

Early Growth Response Protein 1
Transcription factors
Glycerol
Sucrose
Conformations
DNA
Sugar (sucrose)
Proteins
Nucleic Acid Conformation
Polyethylene glycols
Protein Stability
Observation
polyethylene glycol 400

Keywords

  • conformational
  • equilibrium
  • molecular dynamics
  • osmotic stress
  • protein-DNA thermodynamics

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biomaterials
  • Organic Chemistry

Cite this

Mikles, D. C., Bhat, V., Schuchardt, B. J., McDonald, C. B., & Farooq, A. (2015). Effect of osmolytes on the binding of EGR1 transcription factor to DNA. Biopolymers, 103(2), 74-87. https://doi.org/10.1002/bip.22556

Effect of osmolytes on the binding of EGR1 transcription factor to DNA. / Mikles, David C.; Bhat, Vikas; Schuchardt, Brett J.; McDonald, Caleb B.; Farooq, Amjad.

In: Biopolymers, Vol. 103, No. 2, 01.02.2015, p. 74-87.

Research output: Contribution to journalArticle

Mikles, DC, Bhat, V, Schuchardt, BJ, McDonald, CB & Farooq, A 2015, 'Effect of osmolytes on the binding of EGR1 transcription factor to DNA', Biopolymers, vol. 103, no. 2, pp. 74-87. https://doi.org/10.1002/bip.22556
Mikles, David C. ; Bhat, Vikas ; Schuchardt, Brett J. ; McDonald, Caleb B. ; Farooq, Amjad. / Effect of osmolytes on the binding of EGR1 transcription factor to DNA. In: Biopolymers. 2015 ; Vol. 103, No. 2. pp. 74-87.
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