Effect of nucleotides and related compounds on glutamine synthetase activity in chick embryo retina

A biochemical and immunohistochemical study

K. Dutt, Michael D Norenberg, L. Reif-Lehrer

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The effect of a number of nucleotides and related compounds on glutamine synthetase (GS) induction in retina from 12-day chick embryo was studied with both biochemical and immunohistochemical techniques. A number of these compounds gave rise to GS activity comparable to that induced by treatment with cortisol, which is known to give rise to precocious induction of the enzyme in this system. Of the cyclic nucleotides examined, cAMP (0.5 - 1.2 x 10-3 M) gave essentially no increase in GS activity. In contrast, dibutyryl cAMP (0.8 x 10-3 M) had a more significant effect on GS activity, as did 8-bromo-cAMP and cGMP at the same concentration. The activity elicited by these nucleotides was generally half that obtained by treatment with 2.8 x 10-7 M-cortisol for the same length of time. 8-Bromo-cGMP (0.8 x 10-3 M) had an effect comparable to the aforementioned concentration of cortisol. Since phosphodiesterase activity is minimal in the chick retina at 12 days of development, addition of isobutylmethylxanthine (1 x 10-5 M) to this system had, as would be expected, little effect on GS activity. Of the noncyclic compounds, 8-bromoguanosine often gave rise to GS activity comparable to that obtained with cortisol. The other compounds (8-bromo-5'-GMP, guanosine, adenosine, and 5'-AMP) generally had less of an effect on GS. In general, the degree of staining in the immunohistochemical localization of GS corresponded well with the biochemical results and showed the enzyme to be present in regions consistent with the distribution of Muller cells and their processes. Thin-layer chromatography and radioimmunoassay of the nucleotides did not show any steroid impurity in any of the compounds used in the study, even when determinations were carried out at five times the concentration of nucleotide used in the experiments.

Original languageEnglish
Pages (from-to)1239-1244
Number of pages6
JournalJournal of Neurochemistry
Volume36
Issue number3
StatePublished - Jan 1 1981
Externally publishedYes

Fingerprint

Glutamate-Ammonia Ligase
Chick Embryo
Retina
Nucleotides
Hydrocortisone
Guanosine Monophosphate
Ependymoglial Cells
8-Bromo Cyclic Adenosine Monophosphate
Thin layer chromatography
Enzyme Induction
Guanosine
Cyclic Nucleotides
Phosphoric Diester Hydrolases
Enzymes
Adenosine Monophosphate
Thin Layer Chromatography
Adenosine
Radioimmunoassay
Steroids
Impurities

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

@article{57e0e0a4d14c4c9b84a176483f6f9347,
title = "Effect of nucleotides and related compounds on glutamine synthetase activity in chick embryo retina: A biochemical and immunohistochemical study",
abstract = "The effect of a number of nucleotides and related compounds on glutamine synthetase (GS) induction in retina from 12-day chick embryo was studied with both biochemical and immunohistochemical techniques. A number of these compounds gave rise to GS activity comparable to that induced by treatment with cortisol, which is known to give rise to precocious induction of the enzyme in this system. Of the cyclic nucleotides examined, cAMP (0.5 - 1.2 x 10-3 M) gave essentially no increase in GS activity. In contrast, dibutyryl cAMP (0.8 x 10-3 M) had a more significant effect on GS activity, as did 8-bromo-cAMP and cGMP at the same concentration. The activity elicited by these nucleotides was generally half that obtained by treatment with 2.8 x 10-7 M-cortisol for the same length of time. 8-Bromo-cGMP (0.8 x 10-3 M) had an effect comparable to the aforementioned concentration of cortisol. Since phosphodiesterase activity is minimal in the chick retina at 12 days of development, addition of isobutylmethylxanthine (1 x 10-5 M) to this system had, as would be expected, little effect on GS activity. Of the noncyclic compounds, 8-bromoguanosine often gave rise to GS activity comparable to that obtained with cortisol. The other compounds (8-bromo-5'-GMP, guanosine, adenosine, and 5'-AMP) generally had less of an effect on GS. In general, the degree of staining in the immunohistochemical localization of GS corresponded well with the biochemical results and showed the enzyme to be present in regions consistent with the distribution of Muller cells and their processes. Thin-layer chromatography and radioimmunoassay of the nucleotides did not show any steroid impurity in any of the compounds used in the study, even when determinations were carried out at five times the concentration of nucleotide used in the experiments.",
author = "K. Dutt and Norenberg, {Michael D} and L. Reif-Lehrer",
year = "1981",
month = "1",
day = "1",
language = "English",
volume = "36",
pages = "1239--1244",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Effect of nucleotides and related compounds on glutamine synthetase activity in chick embryo retina

T2 - A biochemical and immunohistochemical study

AU - Dutt, K.

AU - Norenberg, Michael D

AU - Reif-Lehrer, L.

PY - 1981/1/1

Y1 - 1981/1/1

N2 - The effect of a number of nucleotides and related compounds on glutamine synthetase (GS) induction in retina from 12-day chick embryo was studied with both biochemical and immunohistochemical techniques. A number of these compounds gave rise to GS activity comparable to that induced by treatment with cortisol, which is known to give rise to precocious induction of the enzyme in this system. Of the cyclic nucleotides examined, cAMP (0.5 - 1.2 x 10-3 M) gave essentially no increase in GS activity. In contrast, dibutyryl cAMP (0.8 x 10-3 M) had a more significant effect on GS activity, as did 8-bromo-cAMP and cGMP at the same concentration. The activity elicited by these nucleotides was generally half that obtained by treatment with 2.8 x 10-7 M-cortisol for the same length of time. 8-Bromo-cGMP (0.8 x 10-3 M) had an effect comparable to the aforementioned concentration of cortisol. Since phosphodiesterase activity is minimal in the chick retina at 12 days of development, addition of isobutylmethylxanthine (1 x 10-5 M) to this system had, as would be expected, little effect on GS activity. Of the noncyclic compounds, 8-bromoguanosine often gave rise to GS activity comparable to that obtained with cortisol. The other compounds (8-bromo-5'-GMP, guanosine, adenosine, and 5'-AMP) generally had less of an effect on GS. In general, the degree of staining in the immunohistochemical localization of GS corresponded well with the biochemical results and showed the enzyme to be present in regions consistent with the distribution of Muller cells and their processes. Thin-layer chromatography and radioimmunoassay of the nucleotides did not show any steroid impurity in any of the compounds used in the study, even when determinations were carried out at five times the concentration of nucleotide used in the experiments.

AB - The effect of a number of nucleotides and related compounds on glutamine synthetase (GS) induction in retina from 12-day chick embryo was studied with both biochemical and immunohistochemical techniques. A number of these compounds gave rise to GS activity comparable to that induced by treatment with cortisol, which is known to give rise to precocious induction of the enzyme in this system. Of the cyclic nucleotides examined, cAMP (0.5 - 1.2 x 10-3 M) gave essentially no increase in GS activity. In contrast, dibutyryl cAMP (0.8 x 10-3 M) had a more significant effect on GS activity, as did 8-bromo-cAMP and cGMP at the same concentration. The activity elicited by these nucleotides was generally half that obtained by treatment with 2.8 x 10-7 M-cortisol for the same length of time. 8-Bromo-cGMP (0.8 x 10-3 M) had an effect comparable to the aforementioned concentration of cortisol. Since phosphodiesterase activity is minimal in the chick retina at 12 days of development, addition of isobutylmethylxanthine (1 x 10-5 M) to this system had, as would be expected, little effect on GS activity. Of the noncyclic compounds, 8-bromoguanosine often gave rise to GS activity comparable to that obtained with cortisol. The other compounds (8-bromo-5'-GMP, guanosine, adenosine, and 5'-AMP) generally had less of an effect on GS. In general, the degree of staining in the immunohistochemical localization of GS corresponded well with the biochemical results and showed the enzyme to be present in regions consistent with the distribution of Muller cells and their processes. Thin-layer chromatography and radioimmunoassay of the nucleotides did not show any steroid impurity in any of the compounds used in the study, even when determinations were carried out at five times the concentration of nucleotide used in the experiments.

UR - http://www.scopus.com/inward/record.url?scp=0019503278&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019503278&partnerID=8YFLogxK

M3 - Article

VL - 36

SP - 1239

EP - 1244

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 3

ER -