Efeito do nitrofen na fase final do desenvolvimento da musculatura do diafragma em ratos

Translated title of the contribution: Effect of nitrofen in the final stages of development of the diaphragm muscle in rats

Frances Lilian Lanhellas Gonçalves, Fábio Santana Oliveira, Augusto Frederico Schmidt, Luís Antônio Violin Dias Pereira, Rodrigo Melo Gallindo, Rebeca Lopes Figueira, Lourenço Sbragia

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


PURPOSE: To evaluate the expression of myosin in muscle fibers of the diaphragm in experimental congenital diaphragmatic hernia (CDH). METHODS: Fetuses of pregnant rats were divided into four groups: External Control (EC), composed of non-manipulated rats; Nitrofen, composed of pregnant rats that received 100 mg of nitrofen (2,4-dichloro-4'nitrodiphenyl ether) diluted in olive oil on gestational day (GD) 9.5, whose fetuses developed CDH (N+) or not (N-), and Olive Oil Placebo (OO), composed of pregnant rats that received the oil on the same GD. The fetuses were collected on GD 18.5, 19.5, 20.5 and 21.5 (term = 22 days). We obtained body weight (BW) and photographed the diaphragm area (DA), hernia area (HA) and subsequent calculated the HA/DA ratio in N+ group. Samples of Diaphragm muscle were processed for histological staining with H/E and immunohistochemistry (IHQ) for myosin. RESULTS: The fetuses of N- and N+ groups had decreased BW and DA compared to EC and OO groups (p <0.001). HA was decreased on GD 18.5 compared to 21.5 (p <0.001) and the HA/DA ratio showed no difference. IHQ showed decreased expression of myosin in nitrofen groups. CONCLUSION: CDH induced by nitrofen model contributes to the understanding of muscularization in the formation of the diaphragm where the myosin expression is decreased.

Translated title of the contributionEffect of nitrofen in the final stages of development of the diaphragm muscle in rats
Original languagePortuguese
Pages (from-to)13-18
Number of pages6
JournalActa Cirurgica Brasileira
Issue numberSUPPL.1
StatePublished - 2013
Externally publishedYes


  • Abnormalities
  • Diaphragm
  • Diaphragmatic
  • Drug-induced
  • Hernia
  • Myosins
  • Rats

ASJC Scopus subject areas

  • Surgery


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