Effect of methotrexate on glial fibrillary acidic protein content of astrocytes in primary culture

Jocelyn H. Bruce-Gregoros, Michael D. Norenberg

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We have recently reported that methotrexate (MTX) causes degenerative as well as reactive-like astroglial changes and alters the cell cycle kinetics of astrocytes in vitro. To further characterize the nature of the reactive-like changes that were noted by light and electron microscopy following MTX exposure, the glial fibrillary acidic protein (GFAP) content of astrocytes in culture was investigated by enzyme-linked immunosorbent assay, flow cytometry and double-immunofluorescent staining. An increase in GFAP content which did not correlate with drug dosage or DNA synthesis was noted in the MTX-treated cultures. It is postulated that this increase in GFAP content of astrocytes reflects an adaptive response to MTX-induced injury and partly explains the gliosis that is seen in methotrexate encephalopathy.

Original languageEnglish (US)
Pages (from-to)118-125
Number of pages8
JournalJournal of Neuropathology and Experimental Neurology
Volume50
Issue number2
DOIs
StatePublished - Mar 1991

Keywords

  • Astrocytes
  • Glial fibrillary acidic protein
  • Methotrexate

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Effect of methotrexate on glial fibrillary acidic protein content of astrocytes in primary culture'. Together they form a unique fingerprint.

  • Cite this