Effect of media and growth promotors on in vitro cultivation of normal murine urinary bladder epithelium

I. Nissenkorn, D. D. Mickey, M. S. Soloway

Research output: Contribution to journalArticle

Abstract

Growth potential of normal murine epithelium was investigated by means of 3 culture media: RPMI 1640 with fetal calf serum Dulbecco's with fetal calf serum and McCoy's with horse serum. The effect of 3 growth promotors was tested separately with each of the 3 media. A total of 886 explants were cultured from 129 murine bladders and epithelial outgrowth was obtained in 35.2 per cent. The growth medium 1640 with 20 per cent fetal calf serum and epithelial growth factor produced the most abundant outgrowth of explants. McCoy's medium containing 15 per cent horse serum produced significantly lower outgrowth compared to the medium containing fetal calf serum (p<0.001). Epidermal growth factor has a stimulating effect and horse serum has an inhibitory effect on growth of normal murine epithelial cells.

Original languageEnglish (US)
Pages (from-to)176-179
Number of pages4
JournalJournal of Urology
Volume128
Issue number1
DOIs
StatePublished - Jan 1 1982

ASJC Scopus subject areas

  • Urology

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