Effect of macrophage secretory products on elaboration of virulence factors by planktonic and biofilm cells of Pseudomonas aeruginosa

Rahul Mittal, Saroj Sharma, Sanjay Chhibber, Kusum Harjai

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Macrophages, which constitute the first line of defense, pour their secretions in the mileu following stimulation with pathogens. These secretory products, referred to as macrophage secretory products (MSPs), can influence ultimate outcome of an infection. In the present investigation, it was observed that different strains of Pseudomonas aeruginosa vary in their ability to stimulate macrophages leading to variability in generation of macrophage secretory products. Cytokine levels, reactive nitrogen intermediates and protein content of macrophage secretory products generated with biofilm cells of P. aeruginosa was found to be more as compared to their planktonic counterparts. The effect of macrophage secretory products produced in response to interaction of macrophages with P. aeruginosa on elaboration of virulence factors produced by planktonic and biofilm cell forms of this pathogen was assessed. Significant enhancement in growth and elaboration of all the virulence determinants by both the cell forms was observed when P. aeruginosa was grown in presence of supernatants with macrophage secretory products. Implications of these findings in relation to urinary tract infections induced by P. aeruginosa have been discussed.

Original languageEnglish (US)
Pages (from-to)12-26
Number of pages15
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume29
Issue number1
DOIs
StatePublished - Jan 2006
Externally publishedYes

Keywords

  • Biofilm cells
  • Macrophage secretory products
  • Pseudomonas aeruginosa
  • Virulence factors

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Immunology
  • veterinary(all)
  • Infectious Diseases

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