Effect of long-term zidovudine exposure on salvage and de novo purine and pyrimidine nucleotide syntheses

Ram P. Agarwal; Jun He; Meena Bansal; Vishal Gupta

doi:10.1016/0167-4889(95)00018-N

Effect of long-term zidovudine exposure on salvage and de novo purine and pyrimidine nucleotide syntheses

Ram P. Agarwal, Jun He, Meena Bansal, Vishal Gupta

Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Salvage and de novo purine and pyrimidine nucleotide syntheses were studied in H⁹ (a human lymphoid cell line) and H_9-AZT cells (chronically zidovudine-exposed H₉ cells). H_9-AZT cells incorporated 18% and 27% more hypoxanthine and uridine, respectively, than H₉ cells. The incorporation of the formate and bicarbonate was similar in both cell lines. Purine and pyrimidine de novo synthesis was inhibited by hypoxanthine and uridine, respectively. Hypoxanthine and uridine salvage pathways, however, were not affected by formate or bicarbonate. Short-term AZT exposure of cells had no effect on nucleotide synthesis. Some of the problems encountered in the studies of purine and pyrimidine synthesis are also discussed.

Original language	English (US)
Pages (from-to)	223-228
Number of pages	6
Journal	BBA - Molecular Cell Research
Volume	1266
Issue number	3
DOIs	https://doi.org/10.1016/0167-4889(95)00018-N
State	Published - May 12 1995

Keywords

Antiviral agent
Purine nucleotide synthesis
Pyrimidine nucleotide synthesis
Salvage and de novo nucleotide synthesis
Zidovudine

ASJC Scopus subject areas

Cell Biology
Molecular Biology
Biophysics

Access to Document

10.1016/0167-4889(95)00018-N

Fingerprint Dive into the research topics of 'Effect of long-term zidovudine exposure on salvage and de novo purine and pyrimidine nucleotide syntheses'. Together they form a unique fingerprint.

Cite this

@article{63b45d883d824aba84f810de9716f8bf,

title = "Effect of long-term zidovudine exposure on salvage and de novo purine and pyrimidine nucleotide syntheses",

abstract = "Salvage and de novo purine and pyrimidine nucleotide syntheses were studied in H9 (a human lymphoid cell line) and H9-AZT cells (chronically zidovudine-exposed H9 cells). H9-AZT cells incorporated 18% and 27% more hypoxanthine and uridine, respectively, than H9 cells. The incorporation of the formate and bicarbonate was similar in both cell lines. Purine and pyrimidine de novo synthesis was inhibited by hypoxanthine and uridine, respectively. Hypoxanthine and uridine salvage pathways, however, were not affected by formate or bicarbonate. Short-term AZT exposure of cells had no effect on nucleotide synthesis. Some of the problems encountered in the studies of purine and pyrimidine synthesis are also discussed.",

keywords = "Antiviral agent, Purine nucleotide synthesis, Pyrimidine nucleotide synthesis, Salvage and de novo nucleotide synthesis, Zidovudine",

author = "Agarwal, {Ram P.} and Jun He and Meena Bansal and Vishal Gupta",

note = "Funding Information: We thank Ms. J. Padmanabhan for technical assistance. We are grateful to Burroughs Wellcome for the gift of zidovudine. This work was supported by the Department of Health and Human Services Grants NIAID AI 29155 and AI 30211.",

year = "1995",

month = may,

day = "12",

doi = "10.1016/0167-4889(95)00018-N",

language = "English (US)",

volume = "1266",

pages = "223--228",

journal = "Biochimica et Biophysica Acta - Molecular Cell Research",

issn = "0167-4889",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Effect of long-term zidovudine exposure on salvage and de novo purine and pyrimidine nucleotide syntheses

AU - Agarwal, Ram P.

AU - He, Jun

AU - Bansal, Meena

AU - Gupta, Vishal

N1 - Funding Information: We thank Ms. J. Padmanabhan for technical assistance. We are grateful to Burroughs Wellcome for the gift of zidovudine. This work was supported by the Department of Health and Human Services Grants NIAID AI 29155 and AI 30211.

PY - 1995/5/12

Y1 - 1995/5/12

N2 - Salvage and de novo purine and pyrimidine nucleotide syntheses were studied in H9 (a human lymphoid cell line) and H9-AZT cells (chronically zidovudine-exposed H9 cells). H9-AZT cells incorporated 18% and 27% more hypoxanthine and uridine, respectively, than H9 cells. The incorporation of the formate and bicarbonate was similar in both cell lines. Purine and pyrimidine de novo synthesis was inhibited by hypoxanthine and uridine, respectively. Hypoxanthine and uridine salvage pathways, however, were not affected by formate or bicarbonate. Short-term AZT exposure of cells had no effect on nucleotide synthesis. Some of the problems encountered in the studies of purine and pyrimidine synthesis are also discussed.

AB - Salvage and de novo purine and pyrimidine nucleotide syntheses were studied in H9 (a human lymphoid cell line) and H9-AZT cells (chronically zidovudine-exposed H9 cells). H9-AZT cells incorporated 18% and 27% more hypoxanthine and uridine, respectively, than H9 cells. The incorporation of the formate and bicarbonate was similar in both cell lines. Purine and pyrimidine de novo synthesis was inhibited by hypoxanthine and uridine, respectively. Hypoxanthine and uridine salvage pathways, however, were not affected by formate or bicarbonate. Short-term AZT exposure of cells had no effect on nucleotide synthesis. Some of the problems encountered in the studies of purine and pyrimidine synthesis are also discussed.

KW - Antiviral agent

KW - Purine nucleotide synthesis

KW - Pyrimidine nucleotide synthesis

KW - Salvage and de novo nucleotide synthesis

KW - Zidovudine

UR - http://www.scopus.com/inward/record.url?scp=0029065249&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029065249&partnerID=8YFLogxK

U2 - 10.1016/0167-4889(95)00018-N

DO - 10.1016/0167-4889(95)00018-N

M3 - Article

C2 - 7766707

AN - SCOPUS:0029065249

VL - 1266

SP - 223

EP - 228

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

SN - 0167-4889

IS - 3

ER -