Effect of long-term zidovudine exposure on salvage and de novo purine and pyrimidine nucleotide syntheses

Ram P. Agarwal, Jun He, Meena Bansal, Vishal Gupta

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Salvage and de novo purine and pyrimidine nucleotide syntheses were studied in H9 (a human lymphoid cell line) and H9-AZT cells (chronically zidovudine-exposed H9 cells). H9-AZT cells incorporated 18% and 27% more hypoxanthine and uridine, respectively, than H9 cells. The incorporation of the formate and bicarbonate was similar in both cell lines. Purine and pyrimidine de novo synthesis was inhibited by hypoxanthine and uridine, respectively. Hypoxanthine and uridine salvage pathways, however, were not affected by formate or bicarbonate. Short-term AZT exposure of cells had no effect on nucleotide synthesis. Some of the problems encountered in the studies of purine and pyrimidine synthesis are also discussed.

Original languageEnglish (US)
Pages (from-to)223-228
Number of pages6
JournalBBA - Molecular Cell Research
Volume1266
Issue number3
DOIs
StatePublished - May 12 1995

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Keywords

  • Antiviral agent
  • Purine nucleotide synthesis
  • Pyrimidine nucleotide synthesis
  • Salvage and de novo nucleotide synthesis
  • Zidovudine

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics

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