Effect of long-term zidovudine exposure on salvage and de novo purine and pyrimidine nucleotide syntheses

Ram P. Agarwal, Jun He, Meena Bansal, Vishal Gupta

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Salvage and de novo purine and pyrimidine nucleotide syntheses were studied in H9 (a human lymphoid cell line) and H9-AZT cells (chronically zidovudine-exposed H9 cells). H9-AZT cells incorporated 18% and 27% more hypoxanthine and uridine, respectively, than H9 cells. The incorporation of the formate and bicarbonate was similar in both cell lines. Purine and pyrimidine de novo synthesis was inhibited by hypoxanthine and uridine, respectively. Hypoxanthine and uridine salvage pathways, however, were not affected by formate or bicarbonate. Short-term AZT exposure of cells had no effect on nucleotide synthesis. Some of the problems encountered in the studies of purine and pyrimidine synthesis are also discussed.

Original languageEnglish
Pages (from-to)223-228
Number of pages6
JournalBBA - Molecular Cell Research
Volume1266
Issue number3
DOIs
StatePublished - May 12 1995
Externally publishedYes

Fingerprint

Pyrimidine Nucleotides
Purine Nucleotides
Zidovudine
formic acid
Hypoxanthine
Uridine
Bicarbonates
Cell Line
Nucleotides
Lymphocytes

Keywords

  • Antiviral agent
  • Purine nucleotide synthesis
  • Pyrimidine nucleotide synthesis
  • Salvage and de novo nucleotide synthesis
  • Zidovudine

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics

Cite this

Effect of long-term zidovudine exposure on salvage and de novo purine and pyrimidine nucleotide syntheses. / Agarwal, Ram P.; He, Jun; Bansal, Meena; Gupta, Vishal.

In: BBA - Molecular Cell Research, Vol. 1266, No. 3, 12.05.1995, p. 223-228.

Research output: Contribution to journalArticle

Agarwal, Ram P. ; He, Jun ; Bansal, Meena ; Gupta, Vishal. / Effect of long-term zidovudine exposure on salvage and de novo purine and pyrimidine nucleotide syntheses. In: BBA - Molecular Cell Research. 1995 ; Vol. 1266, No. 3. pp. 223-228.
@article{63b45d883d824aba84f810de9716f8bf,
title = "Effect of long-term zidovudine exposure on salvage and de novo purine and pyrimidine nucleotide syntheses",
abstract = "Salvage and de novo purine and pyrimidine nucleotide syntheses were studied in H9 (a human lymphoid cell line) and H9-AZT cells (chronically zidovudine-exposed H9 cells). H9-AZT cells incorporated 18{\%} and 27{\%} more hypoxanthine and uridine, respectively, than H9 cells. The incorporation of the formate and bicarbonate was similar in both cell lines. Purine and pyrimidine de novo synthesis was inhibited by hypoxanthine and uridine, respectively. Hypoxanthine and uridine salvage pathways, however, were not affected by formate or bicarbonate. Short-term AZT exposure of cells had no effect on nucleotide synthesis. Some of the problems encountered in the studies of purine and pyrimidine synthesis are also discussed.",
keywords = "Antiviral agent, Purine nucleotide synthesis, Pyrimidine nucleotide synthesis, Salvage and de novo nucleotide synthesis, Zidovudine",
author = "Agarwal, {Ram P.} and Jun He and Meena Bansal and Vishal Gupta",
year = "1995",
month = "5",
day = "12",
doi = "10.1016/0167-4889(95)00018-N",
language = "English",
volume = "1266",
pages = "223--228",
journal = "Biochimica et Biophysica Acta - Molecular Cell Research",
issn = "0167-4889",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Effect of long-term zidovudine exposure on salvage and de novo purine and pyrimidine nucleotide syntheses

AU - Agarwal, Ram P.

AU - He, Jun

AU - Bansal, Meena

AU - Gupta, Vishal

PY - 1995/5/12

Y1 - 1995/5/12

N2 - Salvage and de novo purine and pyrimidine nucleotide syntheses were studied in H9 (a human lymphoid cell line) and H9-AZT cells (chronically zidovudine-exposed H9 cells). H9-AZT cells incorporated 18% and 27% more hypoxanthine and uridine, respectively, than H9 cells. The incorporation of the formate and bicarbonate was similar in both cell lines. Purine and pyrimidine de novo synthesis was inhibited by hypoxanthine and uridine, respectively. Hypoxanthine and uridine salvage pathways, however, were not affected by formate or bicarbonate. Short-term AZT exposure of cells had no effect on nucleotide synthesis. Some of the problems encountered in the studies of purine and pyrimidine synthesis are also discussed.

AB - Salvage and de novo purine and pyrimidine nucleotide syntheses were studied in H9 (a human lymphoid cell line) and H9-AZT cells (chronically zidovudine-exposed H9 cells). H9-AZT cells incorporated 18% and 27% more hypoxanthine and uridine, respectively, than H9 cells. The incorporation of the formate and bicarbonate was similar in both cell lines. Purine and pyrimidine de novo synthesis was inhibited by hypoxanthine and uridine, respectively. Hypoxanthine and uridine salvage pathways, however, were not affected by formate or bicarbonate. Short-term AZT exposure of cells had no effect on nucleotide synthesis. Some of the problems encountered in the studies of purine and pyrimidine synthesis are also discussed.

KW - Antiviral agent

KW - Purine nucleotide synthesis

KW - Pyrimidine nucleotide synthesis

KW - Salvage and de novo nucleotide synthesis

KW - Zidovudine

UR - http://www.scopus.com/inward/record.url?scp=0029065249&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029065249&partnerID=8YFLogxK

U2 - 10.1016/0167-4889(95)00018-N

DO - 10.1016/0167-4889(95)00018-N

M3 - Article

VL - 1266

SP - 223

EP - 228

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

SN - 0167-4889

IS - 3

ER -