Abstract
Salvage and de novo purine and pyrimidine nucleotide syntheses were studied in H9 (a human lymphoid cell line) and H9-AZT cells (chronically zidovudine-exposed H9 cells). H9-AZT cells incorporated 18% and 27% more hypoxanthine and uridine, respectively, than H9 cells. The incorporation of the formate and bicarbonate was similar in both cell lines. Purine and pyrimidine de novo synthesis was inhibited by hypoxanthine and uridine, respectively. Hypoxanthine and uridine salvage pathways, however, were not affected by formate or bicarbonate. Short-term AZT exposure of cells had no effect on nucleotide synthesis. Some of the problems encountered in the studies of purine and pyrimidine synthesis are also discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 223-228 |
| Number of pages | 6 |
| Journal | BBA - Molecular Cell Research |
| Volume | 1266 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 12 1995 |
| Externally published | Yes |
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Keywords
- Antiviral agent
- Purine nucleotide synthesis
- Pyrimidine nucleotide synthesis
- Salvage and de novo nucleotide synthesis
- Zidovudine
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Biophysics
Cite this
Effect of long-term zidovudine exposure on salvage and de novo purine and pyrimidine nucleotide syntheses. / Agarwal, Ram P.; He, Jun; Bansal, Meena; Gupta, Vishal.
In: BBA - Molecular Cell Research, Vol. 1266, No. 3, 12.05.1995, p. 223-228.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of long-term zidovudine exposure on salvage and de novo purine and pyrimidine nucleotide syntheses
AU - Agarwal, Ram P.
AU - He, Jun
AU - Bansal, Meena
AU - Gupta, Vishal
PY - 1995/5/12
Y1 - 1995/5/12
N2 - Salvage and de novo purine and pyrimidine nucleotide syntheses were studied in H9 (a human lymphoid cell line) and H9-AZT cells (chronically zidovudine-exposed H9 cells). H9-AZT cells incorporated 18% and 27% more hypoxanthine and uridine, respectively, than H9 cells. The incorporation of the formate and bicarbonate was similar in both cell lines. Purine and pyrimidine de novo synthesis was inhibited by hypoxanthine and uridine, respectively. Hypoxanthine and uridine salvage pathways, however, were not affected by formate or bicarbonate. Short-term AZT exposure of cells had no effect on nucleotide synthesis. Some of the problems encountered in the studies of purine and pyrimidine synthesis are also discussed.
AB - Salvage and de novo purine and pyrimidine nucleotide syntheses were studied in H9 (a human lymphoid cell line) and H9-AZT cells (chronically zidovudine-exposed H9 cells). H9-AZT cells incorporated 18% and 27% more hypoxanthine and uridine, respectively, than H9 cells. The incorporation of the formate and bicarbonate was similar in both cell lines. Purine and pyrimidine de novo synthesis was inhibited by hypoxanthine and uridine, respectively. Hypoxanthine and uridine salvage pathways, however, were not affected by formate or bicarbonate. Short-term AZT exposure of cells had no effect on nucleotide synthesis. Some of the problems encountered in the studies of purine and pyrimidine synthesis are also discussed.
KW - Antiviral agent
KW - Purine nucleotide synthesis
KW - Pyrimidine nucleotide synthesis
KW - Salvage and de novo nucleotide synthesis
KW - Zidovudine
UR - http://www.scopus.com/inward/record.url?scp=0029065249&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029065249&partnerID=8YFLogxK
U2 - 10.1016/0167-4889(95)00018-N
DO - 10.1016/0167-4889(95)00018-N
M3 - Article
VL - 1266
SP - 223
EP - 228
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
SN - 0167-4889
IS - 3
ER -