TY - JOUR
T1 - Effect of long-term zidovudine exposure on salvage and de novo purine and pyrimidine nucleotide syntheses
AU - Agarwal, Ram P.
AU - He, Jun
AU - Bansal, Meena
AU - Gupta, Vishal
N1 - Funding Information:
We thank Ms. J. Padmanabhan for technical assistance. We are grateful to Burroughs Wellcome for the gift of zidovudine. This work was supported by the Department of Health and Human Services Grants NIAID AI 29155 and AI 30211.
PY - 1995/5/12
Y1 - 1995/5/12
N2 - Salvage and de novo purine and pyrimidine nucleotide syntheses were studied in H9 (a human lymphoid cell line) and H9-AZT cells (chronically zidovudine-exposed H9 cells). H9-AZT cells incorporated 18% and 27% more hypoxanthine and uridine, respectively, than H9 cells. The incorporation of the formate and bicarbonate was similar in both cell lines. Purine and pyrimidine de novo synthesis was inhibited by hypoxanthine and uridine, respectively. Hypoxanthine and uridine salvage pathways, however, were not affected by formate or bicarbonate. Short-term AZT exposure of cells had no effect on nucleotide synthesis. Some of the problems encountered in the studies of purine and pyrimidine synthesis are also discussed.
AB - Salvage and de novo purine and pyrimidine nucleotide syntheses were studied in H9 (a human lymphoid cell line) and H9-AZT cells (chronically zidovudine-exposed H9 cells). H9-AZT cells incorporated 18% and 27% more hypoxanthine and uridine, respectively, than H9 cells. The incorporation of the formate and bicarbonate was similar in both cell lines. Purine and pyrimidine de novo synthesis was inhibited by hypoxanthine and uridine, respectively. Hypoxanthine and uridine salvage pathways, however, were not affected by formate or bicarbonate. Short-term AZT exposure of cells had no effect on nucleotide synthesis. Some of the problems encountered in the studies of purine and pyrimidine synthesis are also discussed.
KW - Antiviral agent
KW - Purine nucleotide synthesis
KW - Pyrimidine nucleotide synthesis
KW - Salvage and de novo nucleotide synthesis
KW - Zidovudine
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U2 - 10.1016/0167-4889(95)00018-N
DO - 10.1016/0167-4889(95)00018-N
M3 - Article
C2 - 7766707
AN - SCOPUS:0029065249
VL - 1266
SP - 223
EP - 228
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
SN - 0167-4889
IS - 3
ER -