Effect of ionic strength and temperature on the protonation of oxidized glutathione

Pasquale Crea, Concetta De Stefano, Mutsa Kambarami, Frank J. Millero, Virender K. Sharma

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The protonation constants for oxidized glutathione, Hi-1L (4-i+1)-, KiH =[HiL (4-i)-]/[Hi-1L(4-i+1)-][H+] i=1,2,...,6 have been measured at 5, 25 and 45°C as a function of the ionic strength (0.1 to 5.4 mol·[kg(H2O)]-1) in NaCl solutions. The effect of ionic strength on the measured protonation constants has been used to determine the thermodynamic values (KiH0 ) and the enthalpy (ΔHi ) for the dissociation reaction using the SIT model and Pitzer equations. The SIT (ε) and Pitzer parameters (β(0), β(1) and C) for the dissociation products (L4-, HL3-, H2L2-, H 3L-, H4L, H5L+, H 6L2+) have been determined as a function of temperature. These results can be used to examine the effect of ionic strength and temperature on glutathione in aqueous solutions with NaCl as the major component (body fluids, seawater and brines).

Original languageEnglish (US)
Pages (from-to)1245-1259
Number of pages15
JournalJournal of Solution Chemistry
Issue number9
StatePublished - Sep 2008


  • Ionic strength
  • Oxidized glutathione
  • Pitzer coefficients
  • Protonation constants
  • SIT model
  • Sodium chloride

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Physical and Theoretical Chemistry


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