Effect of ionic strength and temperature on the protonation of oxidized glutathione

Pasquale Crea; Concetta De Stefano; Mutsa Kambarami; Frank J. Millero; Virender K. Sharma

doi:10.1007/s10953-008-9310-2

Effect of ionic strength and temperature on the protonation of oxidized glutathione

Pasquale Crea, Concetta De Stefano, Mutsa Kambarami, Frank J. Millero, Virender K. Sharma

Ocean Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The protonation constants for oxidized glutathione, H_i-1L ^(4-i+1)-, K_i^H =[H_iL ^(4-i)-]/[H_i-1L^(4-i+1)-][H⁺] i=1,2,...,6 have been measured at 5, 25 and 45°C as a function of the ionic strength (0.1 to 5.4 mol·[kg(H₂O)]^-1) in NaCl solutions. The effect of ionic strength on the measured protonation constants has been used to determine the thermodynamic values (K_i^H0 ) and the enthalpy (ΔH_i ) for the dissociation reaction using the SIT model and Pitzer equations. The SIT (ε) and Pitzer parameters (β⁽⁰⁾, β⁽¹⁾ and C) for the dissociation products (L^4-, HL^3-, H₂L^2-, H ₃L^-, H₄L, H₅L⁺, H ₆L²⁺) have been determined as a function of temperature. These results can be used to examine the effect of ionic strength and temperature on glutathione in aqueous solutions with NaCl as the major component (body fluids, seawater and brines).

Original language	English (US)
Pages (from-to)	1245-1259
Number of pages	15
Journal	Journal of Solution Chemistry
Volume	37
Issue number	9
DOIs	https://doi.org/10.1007/s10953-008-9310-2
State	Published - Sep 1 2008

Keywords

Ionic strength
Oxidized glutathione
Pitzer coefficients
Protonation constants
SIT model
Sodium chloride

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Physical and Theoretical Chemistry

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title = "Effect of ionic strength and temperature on the protonation of oxidized glutathione",

abstract = "The protonation constants for oxidized glutathione, Hi-1L (4-i+1)-, KiH =[HiL (4-i)-]/[Hi-1L(4-i+1)-][H+] i=1,2,...,6 have been measured at 5, 25 and 45°C as a function of the ionic strength (0.1 to 5.4 mol·[kg(H2O)]-1) in NaCl solutions. The effect of ionic strength on the measured protonation constants has been used to determine the thermodynamic values (KiH0 ) and the enthalpy (ΔHi ) for the dissociation reaction using the SIT model and Pitzer equations. The SIT (ε) and Pitzer parameters (β(0), β(1) and C) for the dissociation products (L4-, HL3-, H2L2-, H 3L-, H4L, H5L+, H 6L2+) have been determined as a function of temperature. These results can be used to examine the effect of ionic strength and temperature on glutathione in aqueous solutions with NaCl as the major component (body fluids, seawater and brines).",

keywords = "Ionic strength, Oxidized glutathione, Pitzer coefficients, Protonation constants, SIT model, Sodium chloride",

author = "Pasquale Crea and {De Stefano}, Concetta and Mutsa Kambarami and Millero, {Frank J.} and Sharma, {Virender K.}",

year = "2008",

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doi = "10.1007/s10953-008-9310-2",

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T1 - Effect of ionic strength and temperature on the protonation of oxidized glutathione

AU - Crea, Pasquale

AU - De Stefano, Concetta

AU - Kambarami, Mutsa

AU - Millero, Frank J.

AU - Sharma, Virender K.

PY - 2008/9/1

Y1 - 2008/9/1

N2 - The protonation constants for oxidized glutathione, Hi-1L (4-i+1)-, KiH =[HiL (4-i)-]/[Hi-1L(4-i+1)-][H+] i=1,2,...,6 have been measured at 5, 25 and 45°C as a function of the ionic strength (0.1 to 5.4 mol·[kg(H2O)]-1) in NaCl solutions. The effect of ionic strength on the measured protonation constants has been used to determine the thermodynamic values (KiH0 ) and the enthalpy (ΔHi ) for the dissociation reaction using the SIT model and Pitzer equations. The SIT (ε) and Pitzer parameters (β(0), β(1) and C) for the dissociation products (L4-, HL3-, H2L2-, H 3L-, H4L, H5L+, H 6L2+) have been determined as a function of temperature. These results can be used to examine the effect of ionic strength and temperature on glutathione in aqueous solutions with NaCl as the major component (body fluids, seawater and brines).

AB - The protonation constants for oxidized glutathione, Hi-1L (4-i+1)-, KiH =[HiL (4-i)-]/[Hi-1L(4-i+1)-][H+] i=1,2,...,6 have been measured at 5, 25 and 45°C as a function of the ionic strength (0.1 to 5.4 mol·[kg(H2O)]-1) in NaCl solutions. The effect of ionic strength on the measured protonation constants has been used to determine the thermodynamic values (KiH0 ) and the enthalpy (ΔHi ) for the dissociation reaction using the SIT model and Pitzer equations. The SIT (ε) and Pitzer parameters (β(0), β(1) and C) for the dissociation products (L4-, HL3-, H2L2-, H 3L-, H4L, H5L+, H 6L2+) have been determined as a function of temperature. These results can be used to examine the effect of ionic strength and temperature on glutathione in aqueous solutions with NaCl as the major component (body fluids, seawater and brines).

KW - Ionic strength

KW - Oxidized glutathione

KW - Pitzer coefficients

KW - Protonation constants

KW - SIT model

KW - Sodium chloride

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