Effect of interleukin-2 and interleukin-4 on lymphocytes from peribronchial lymphatic tissue

Reuven Or, Izidore S. Lossos, Esther Hirschfeld, Raphael Breuer

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Lymphocyte activation in spleen and peribronchial lymphatic tissue (PBLT) following stimulation with T-cell mitogens and lymphokines was investigated in the hamster. Optimal mitogen-induced cell proliferation was achieved after culturing for 6 days in vitro. Interleukin-2 (IL-2) and interleukin-4 (IL-4) did not induce DNA synthesis in resting T-cells from either spleen or PBLT. IL-2 or IL-4 in combination with concanavalin A promoted splenic T-cell proliferation. In contrast, in PBLT, IL-2-but not IL-4-enhanced cell proliferation (p < .001). The findings indicate that PBLT represents an independent compartment of the immune system. Furthermore, in the hamster PBLT cells consist predominantly of IL-2-responsive cells, i.e., are of the Th1 type. Immunological pathogenesis of lung injury can therefore be studied by functional analysis of PBLT lymphocytes.

Original languageEnglish (US)
Pages (from-to)245-253
Number of pages9
JournalExperimental Lung Research
Volume22
Issue number2
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Keywords

  • hamster
  • immune system activation
  • lymphocytes
  • peribronchial lymphatic tissue
  • T-cells

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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