Effect of interleukin-2 and interleukin-4 on lymphocytes from peribronchial lymphatic tissue

Reuven Or, Izidore Lossos, Esther Hirschfeld, Raphael Breuer

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Lymphocyte activation in spleen and peribronchial lymphatic tissue (PBLT) following stimulation with T-cell mitogens and lymphokines was investigated in the hamster. Optimal mitogen-induced cell proliferation was achieved after culturing for 6 days in vitro. Interleukin-2 (IL-2) and interleukin-4 (IL-4) did not induce DNA synthesis in resting T-cells from either spleen or PBLT. IL-2 or IL-4 in combination with concanavalin A promoted splenic T-cell proliferation. In contrast, in PBLT, IL-2-but not IL-4-enhanced cell proliferation (p < .001). The findings indicate that PBLT represents an independent compartment of the immune system. Furthermore, in the hamster PBLT cells consist predominantly of IL-2-responsive cells, i.e., are of the Th1 type. Immunological pathogenesis of lung injury can therefore be studied by functional analysis of PBLT lymphocytes.

Original languageEnglish
Pages (from-to)245-253
Number of pages9
JournalExperimental Lung Research
Volume22
Issue number2
StatePublished - Apr 11 1996
Externally publishedYes

Fingerprint

Lymphocytes
Lymphoid Tissue
Interleukin-4
Interleukin-2
Tissue
T-cells
Cell proliferation
Cell Proliferation
T-Lymphocytes
Mitogens
Cricetinae
Spleen
Functional analysis
Th1 Cells
Lymphokines
Immune system
Lung Injury
Lymphocyte Activation
Concanavalin A
Immune System

Keywords

  • hamster
  • immune system activation
  • lymphocytes
  • peribronchial lymphatic tissue
  • T-cells

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Effect of interleukin-2 and interleukin-4 on lymphocytes from peribronchial lymphatic tissue. / Or, Reuven; Lossos, Izidore; Hirschfeld, Esther; Breuer, Raphael.

In: Experimental Lung Research, Vol. 22, No. 2, 11.04.1996, p. 245-253.

Research output: Contribution to journalArticle

Or, Reuven ; Lossos, Izidore ; Hirschfeld, Esther ; Breuer, Raphael. / Effect of interleukin-2 and interleukin-4 on lymphocytes from peribronchial lymphatic tissue. In: Experimental Lung Research. 1996 ; Vol. 22, No. 2. pp. 245-253.
@article{046262632ebf4e2fb84d2c7416c4818e,
title = "Effect of interleukin-2 and interleukin-4 on lymphocytes from peribronchial lymphatic tissue",
abstract = "Lymphocyte activation in spleen and peribronchial lymphatic tissue (PBLT) following stimulation with T-cell mitogens and lymphokines was investigated in the hamster. Optimal mitogen-induced cell proliferation was achieved after culturing for 6 days in vitro. Interleukin-2 (IL-2) and interleukin-4 (IL-4) did not induce DNA synthesis in resting T-cells from either spleen or PBLT. IL-2 or IL-4 in combination with concanavalin A promoted splenic T-cell proliferation. In contrast, in PBLT, IL-2-but not IL-4-enhanced cell proliferation (p < .001). The findings indicate that PBLT represents an independent compartment of the immune system. Furthermore, in the hamster PBLT cells consist predominantly of IL-2-responsive cells, i.e., are of the Th1 type. Immunological pathogenesis of lung injury can therefore be studied by functional analysis of PBLT lymphocytes.",
keywords = "hamster, immune system activation, lymphocytes, peribronchial lymphatic tissue, T-cells",
author = "Reuven Or and Izidore Lossos and Esther Hirschfeld and Raphael Breuer",
year = "1996",
month = "4",
day = "11",
language = "English",
volume = "22",
pages = "245--253",
journal = "Experimental Lung Research",
issn = "0190-2148",
publisher = "Informa Healthcare",
number = "2",

}

TY - JOUR

T1 - Effect of interleukin-2 and interleukin-4 on lymphocytes from peribronchial lymphatic tissue

AU - Or, Reuven

AU - Lossos, Izidore

AU - Hirschfeld, Esther

AU - Breuer, Raphael

PY - 1996/4/11

Y1 - 1996/4/11

N2 - Lymphocyte activation in spleen and peribronchial lymphatic tissue (PBLT) following stimulation with T-cell mitogens and lymphokines was investigated in the hamster. Optimal mitogen-induced cell proliferation was achieved after culturing for 6 days in vitro. Interleukin-2 (IL-2) and interleukin-4 (IL-4) did not induce DNA synthesis in resting T-cells from either spleen or PBLT. IL-2 or IL-4 in combination with concanavalin A promoted splenic T-cell proliferation. In contrast, in PBLT, IL-2-but not IL-4-enhanced cell proliferation (p < .001). The findings indicate that PBLT represents an independent compartment of the immune system. Furthermore, in the hamster PBLT cells consist predominantly of IL-2-responsive cells, i.e., are of the Th1 type. Immunological pathogenesis of lung injury can therefore be studied by functional analysis of PBLT lymphocytes.

AB - Lymphocyte activation in spleen and peribronchial lymphatic tissue (PBLT) following stimulation with T-cell mitogens and lymphokines was investigated in the hamster. Optimal mitogen-induced cell proliferation was achieved after culturing for 6 days in vitro. Interleukin-2 (IL-2) and interleukin-4 (IL-4) did not induce DNA synthesis in resting T-cells from either spleen or PBLT. IL-2 or IL-4 in combination with concanavalin A promoted splenic T-cell proliferation. In contrast, in PBLT, IL-2-but not IL-4-enhanced cell proliferation (p < .001). The findings indicate that PBLT represents an independent compartment of the immune system. Furthermore, in the hamster PBLT cells consist predominantly of IL-2-responsive cells, i.e., are of the Th1 type. Immunological pathogenesis of lung injury can therefore be studied by functional analysis of PBLT lymphocytes.

KW - hamster

KW - immune system activation

KW - lymphocytes

KW - peribronchial lymphatic tissue

KW - T-cells

UR - http://www.scopus.com/inward/record.url?scp=0029921877&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029921877&partnerID=8YFLogxK

M3 - Article

C2 - 8706639

AN - SCOPUS:0029921877

VL - 22

SP - 245

EP - 253

JO - Experimental Lung Research

JF - Experimental Lung Research

SN - 0190-2148

IS - 2

ER -