Effect of hypothalamic proline-rich peptide (PRP-1) on neuronal and bone marrow cell apoptosis

Armen A. Galoyan, Josef Krieglstein, Susanne Klumpp, Kristina E. Danielian, Karine A. Galoian, Wolfram Kremers, Kristina B. Bezirganyan, Tigran K. Davtyan

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The AGAPEPAEPAQPGVY proline-rich peptide (PRP-1) was isolated from neurosecretory granules of the bovine neurohypophysis; it is produced by N. supraopticus and N. paraventricularis. It has been shown that PRP-1 has many potentially beneficial biological effects including immunoregulatory, hematopoietic, antimicrobial and anti-neurodegenerative properties. Here we investigated the influence of PRP-1 on staurosporine-induced apoptosis of postnatal hippocampal cells and on doxorubicin-induced bone marrow granulocyte- and monocyte apoptosis. The intention was to further characterize the effect of PRP-1 on the survival rate of neurons and in context with myelopoiesis. We demonstrate that PRP-1 significantly reduced apoptosis of postnatal hippocampal cells induced by staurosporine. The protective effect of PRP-1 against apoptotic cell death was shown to be both time- and dose-dependent. Neuroprotection was more pronounced after prolonged pretreatment of the cells with PRP-1 before the induction of apoptosis with staurosporine. The related peptide [arg 8]vasopressin did not reveal neuroprotection. PRP-1 also significantly reduced apoptosis of bone marrow monocytes and granulocytes induced by doxorubicin. This protective effect lasted for 2-4 h and was not detectable anymore after 24 h when PRP-1 and doxorubicin were added simultaneously. Previously obtained data and results of the current studies suggested that the hypothalamic PRP-1 possibly represents an endogenous peptide whose primary functions are to regulate myelopoiesis and neuron survival as we provide evidence that PRP can differentially reduce both staurosporine- and doxorubicin-induced hippocampal and bone marrow cell apoptosis.

Original languageEnglish (US)
Pages (from-to)1898-1905
Number of pages8
JournalNeurochemical Research
Volume32
Issue number11
DOIs
StatePublished - Nov 2007

Keywords

  • Bone marrow granulocytes
  • Hypothalamic proline-rich polypeptide
  • Postnatal hippocampal cells
  • Staurosporine- and doxorubicin-induced apoptosis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

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