Effect of human immunodeficiency virus type-1 envelope glycoprotein gp160 on cytokine production from cord-blood T cells

Soe Than, Naoki Oyaizu, Rajendra Pahwa, Vaniambadi S. Kalyanaraman, Savita G Pahwa

Research output: Contribution to journalArticle

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Abstract

We have recently demonstrated that the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein gp160 enhances the in vitro differentiation of hematopoietic myeloid progenitor cells derived from cord blood by inducing secretion of colony-stimulating factor(s) (CSF) in T cells, presumably through the interaction of gp160 with CD4 molecules. In this study, we investigated the gp160-induced humoral CSFs in cord blood by enzyme-linked immunosorbent assay (ELISA) and by polymerase chain reaction on reverse- transcribed mRNA (RT-PCR). We demonstrate that gp160 can induce interleukin (IL)-3, IL-6, and granulocyte-macrophage CSF (GM-CSF) protein secretion only in purified cord-blood T cells (CB-T) and not in detectable amounts in whole cord blood cells (WCB); cytokine mRNA induction occurred in purified CB-T and WCB, but was significantly greater in the former. Treatment of gp160 with soluble CD4 (sCD4) abolished the secretion of all three cytokines in CB-T cells, which suggests that interaction of gp160 with CD4 molecules is required for the secretion of these cytokines from CB-T cells. However, in WCB cells, sCD4 treatment of gp160 resulted in inhibition of only IL-3 and GM-CSF mRNA, whereas IL-6 secretion was enhanced. Purified cord-blood monocytes secreted only IL-6 in response to gp160, and the gp160-induced IL- 6 secretion by monocytes was also further increased by gp160 + sCD4 complex. Furthermore, monocyte culture supernatants suppressed gp160-induced IL-3 secretion from CB-T cells. These findings indicate that (1) CB-T cells are a potent source of gp160-induced hematopoietic cytokines, and (2) that different mechanisms are involved in the induction of IL-6 by gp160 in the T- and non-T-cell fractions of cord blood. The ability of HIV gp160 to induce hematopoietic CSFs in cord blood may be important in HIV pathogenesis.

Original languageEnglish
Pages (from-to)184-188
Number of pages5
JournalBlood
Volume84
Issue number1
StatePublished - Jul 1 1994
Externally publishedYes

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T-cells
Fetal Blood
Viruses
HIV-1
Blood Cells
Glycoproteins
Blood
Cytokines
T-Lymphocytes
Cells
Interleukin-6
Interleukin-3
Monocytes
CD4 Antigens
Macrophages
Granulocytes
Messenger RNA
HIV Envelope Protein gp160
Myeloid Progenitor Cells
Colony-Stimulating Factors

ASJC Scopus subject areas

  • Hematology

Cite this

Effect of human immunodeficiency virus type-1 envelope glycoprotein gp160 on cytokine production from cord-blood T cells. / Than, Soe; Oyaizu, Naoki; Pahwa, Rajendra; Kalyanaraman, Vaniambadi S.; Pahwa, Savita G.

In: Blood, Vol. 84, No. 1, 01.07.1994, p. 184-188.

Research output: Contribution to journalArticle

Than, Soe ; Oyaizu, Naoki ; Pahwa, Rajendra ; Kalyanaraman, Vaniambadi S. ; Pahwa, Savita G. / Effect of human immunodeficiency virus type-1 envelope glycoprotein gp160 on cytokine production from cord-blood T cells. In: Blood. 1994 ; Vol. 84, No. 1. pp. 184-188.
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