Effect of human immunodeficiency virus-1 envelope glycoprotein on in vitro hematopoiesis of umbilical cord blood

Kikuya Sugiura, Naoki Oyaizu, Rajendra Pahwa, Vaniambadi S. Kalyanaraman, Savita G Pahwa

Research output: Contribution to journalArticle

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Abstract

Although hypercellularity is a common bone marrow finding in patients with human immunodeficiency virus type 1 (HIV-1) infection, the effect of HIV-1 on the hematopoietic system, which has been investigated in in vitro studies, is still controversial. In this study, we have investigated the effects of HIV-1 envelope glycoprotein, gp160, on the differentiation of hematopoietic progenitor cells derived from cord blood. Culture of cord blood mononuclear cells with gp160 resulted in enhancement of the in vitro growth of myeloid hematopoietic progenitors. To investigate the mechanism of the enhancement, adherent cells, T cells, or CD34-bearing hematopoietic progenitors were isolated and cultivated with gp160 in a variety of culture conditions. We have shown that gp160 had no direct effect on highly purified hematopoietic progenitors but exerted its enhancing effect indirectly via T cells, by induction of a humoral colony-stimulating factor(s). The activity of gp160 on T cells was abrogated by preincubation of gp160 with recombinant CD4 molecule and goat anti-gp120 antibody. These data provide evidence for a novel biologic activity of HIV envelope glycoprotein, that of T-cell-mediated stimulation of myelopoiesis. Binding of gp160 with the cell surface CD4 molecule appears to be necessary for secretion of the colony-stimulating factor(s).

Original languageEnglish
Pages (from-to)1463-1469
Number of pages7
JournalBlood
Volume80
Issue number6
StatePublished - Sep 15 1992
Externally publishedYes

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T-cells
Hematopoiesis
Fetal Blood
Viruses
HIV-1
Glycoproteins
Blood
T-Lymphocytes
Colony-Stimulating Factors
CD4 Antigens
Bearings (structural)
Myelopoiesis
Hematopoietic System
Virus Diseases
Hematopoietic Stem Cells
Goats
Anti-Idiotypic Antibodies
Blood Cells
Bone
Bone Marrow

ASJC Scopus subject areas

  • Hematology

Cite this

Effect of human immunodeficiency virus-1 envelope glycoprotein on in vitro hematopoiesis of umbilical cord blood. / Sugiura, Kikuya; Oyaizu, Naoki; Pahwa, Rajendra; Kalyanaraman, Vaniambadi S.; Pahwa, Savita G.

In: Blood, Vol. 80, No. 6, 15.09.1992, p. 1463-1469.

Research output: Contribution to journalArticle

Sugiura, K, Oyaizu, N, Pahwa, R, Kalyanaraman, VS & Pahwa, SG 1992, 'Effect of human immunodeficiency virus-1 envelope glycoprotein on in vitro hematopoiesis of umbilical cord blood', Blood, vol. 80, no. 6, pp. 1463-1469.
Sugiura, Kikuya ; Oyaizu, Naoki ; Pahwa, Rajendra ; Kalyanaraman, Vaniambadi S. ; Pahwa, Savita G. / Effect of human immunodeficiency virus-1 envelope glycoprotein on in vitro hematopoiesis of umbilical cord blood. In: Blood. 1992 ; Vol. 80, No. 6. pp. 1463-1469.
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