Effect of high dose per pulse flattening filter-free beams on cancer cell survival

Ines Lohse, Stephanie Lang, Jan Hrbacek, Stephan Scheidegger, Stephan Bodis, Nadia S. MacEdo, Jianhua Feng, Urs M. Lütolf, Kathrin Zaugg

Research output: Contribution to journalArticlepeer-review

66 Scopus citations


Purpose: To investigate if there is a statistically significant difference in cancer cell survival using a high dose per pulse flattening filter-free (FFF) beam compared to a standard flattened beam. Material and methods: To validate the radiobiological effect of the flattened and FFF beam, two glioblastoma cell lines were treated with either 5 or 10 Gy using different dose rates. Dose verification was performed and colony formation assays were carried out. To compare the predictability of our data, radiobiological models were included. Results: The results presented here demonstrate that irradiation of glioblastoma cell lines using the FFF beam is more efficient in reducing clonogenic cell survival than the standard flattened beam, an effect which becomes more significant the higher the single dose. Interestingly, in our experimental setting, the radiobiological effect of the FFF beam is dependent on dose per pulse rather than on delivery time. The used radiobiological models are able to describe the observed dose rate dependency between 6 and 24 Gy/min. Conclusion: The results presented here show that dose per pulse might become a crucial factor which influences cancer cell survival. Using high dose rates, currently used radiobiological models as well as molecular mechanisms involved urgently need to be re-examined.

Original languageEnglish (US)
Pages (from-to)226-232
Number of pages7
JournalRadiotherapy and Oncology
Issue number1
StatePublished - Oct 2011
Externally publishedYes


  • Cancer cell survival
  • Dose rate
  • Flattening filter-free
  • Ionizing radiation

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Radiology Nuclear Medicine and imaging


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