Wirkung verschiedener wundspüllösungen auf MRSA-biofilme in wunden im tiermodell (schwein)

Translated title of the contribution: Effect of different wound rinsing solutions on MRSA biofilm in a porcine wound model

Roberto Perez, Stephen C. Davis, Kurt Kaehn

Research output: Contribution to journalArticle

Abstract

Background: The impact of biofilm for hard to heal wounds is more and more perceived and discussed. Methicilline resistant Staphylococcus aureus (MRSA) is a competent biofilm producer and often isolated from chronic wounds. Removal of biofilm from wounds can improve healing and additionally contains transmission of MRSA. Using a porcine wound model the effectiveness of a routine wound care procedure - wound cleansing by rinsing - was tested for biofilm removal from skin wounds. Method: Partial thickness wounds on the back of pigs were spiked with MRSA and covered with polyurethane dressings for 24 hours to allow growths of biofilm. The wounds were then assigned to four groups. In three groups the wounds were cleansed twice a day by rinsing using i) a special wound rinsing solution containing betaine and polyhexanide, ii) Ringer's solution, and iii) sterile saline. The wounds in the control group were not rinsed. Four wounds from each group were cultured at 48 and 72 hours respectively. Results: Means of MRSA counts at 48 and 72 hours were significantly reduced (p < 0.05) in group i) compared to group ii) and iii). Conclusion: Removal of MRSA biofilm was only demonstrated using the betaine and polyhexanide containing wound rinsing solution; both salt solutions failed to reduce MRSA counts.

Original languageGerman
Pages (from-to)464-468
Number of pages5
JournalHygiene + Medizin
Volume35
Issue number12
StatePublished - Dec 1 2010

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Keywords

  • Betaine
  • Biofilm
  • Polyhexanide
  • Porcine wound model
  • Wound rinsing

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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