Effect of Different Binding Proteins on the Detection Limits and Sensitivity of Assays Based on Biotinylated Adenosine Deaminase

Minas S. Barbarakis, Sylvia Daunert, Leonidas G. Bachas

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The properties of binding proteins that control the nature and magnitude of inhibition of the enzyme-ligand conjugates in homogeneous enzyme-linked competitive binding assays were investigated. An assay for biotin that employed adenosine deaminase as the enzyme-label was used as a model system because of the availability of several biotin-specific binders with different characteristics. It was found that the association constant between the ligand and the binding protein, as well as the depth of the binding pocket, affect the response characteristics of the assay. In addition, the reported data suggest that steric-hindrance effects also contribute toward the sensitivity and detection-limit capabilities of the assay.

Original languageEnglish (US)
Pages (from-to)225-229
Number of pages5
JournalBioconjugate Chemistry
Volume3
Issue number3
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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