Effect of cyclic AMP on ammonia-induced alterations in primary astrocyte cultures

Jocelyn B. Gregorios, Lee W. Mozes, Luz Oliva B. Norenberg, Michael D. Norenberg

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Current evidence suggests that astrocytes may be the target of ammonia toxicity. Consistent with this view are recent investigations which have shown morphologic alterations in primary astrocyte cultures following exposure to ammonia. In the present study, these alterations became severely aggravated when the cultures were not grown or maintained in dibutyryl cyclic adenosine monophosphate (AMP). Cyclic AMP analogues and agents that increase intracellular cyclic AMP levels significantly inhibited the toxic effects of ammonia. The exact mechanism responsible for this apparent protective effect of cyclic AMP on ammonia-treated astrocytes is not known. The possible means by which cyclic AMP may serve to ameliorate ammonia-induced toxicity are discussed.

Original languageEnglish (US)
Pages (from-to)396-407
Number of pages12
JournalJournal of Neuropathology and Experimental Neurology
Issue number4
StatePublished - Jul 1986


  • Ammonia
  • Astrocytes
  • Cyclic adenosine monophosphate
  • Tissue culture

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience


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