Effect of chronic, low-pressure, sterile vesicoureteral reflux on renal growth and function in a porcine model: A radiologic and pathologic study

H. J. Paltiel, R. V. Mulkern, A. Perez-Atayde, L. P. Connolly, D. Zurakowski, S. Ted Treves, A. Atala

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

PURPOSE: To study the effects of chronic, low-pressure, sterile vesicoureteral reflux (VUR) on renal growth and function in a porcine model. MATERIALS AND METHODS: Unilateral VUR was created in five pigs, with the contralateral kidney serving as a control. Preoperatively, and 1 year later, ultrasonography, technetium 99m-dimercaptosuccinic acid (DMSA) scintigraphy, contrast material-enhanced computed tomography (CT), and contrast-enhanced magnetic resonance (MR) imaging were performed. Morphologic abnormalities and relative uptake of 99mTc-DMSA were recorded. The postcontrast enhancement ratios for parenchymal regions of interest at CT and MR imaging were determined. Ruthenium 103-labeled microspheres were used to determine regional blood flow. After the pigs were sacrificed, the kidneys were excised, weighed, and analyzed pathologically. RESULTS: Two of five refluxing kidneys had less than 45% function at scintigraphy. One of these two kidneys was small at postmortem examination. There were no other imaging or gross pathologic abnormalities. There was no significant difference in regional blood flow between the refluxing and nonrefluxing kidneys. In all of the operated on kidneys, histologic examination showed focal chronic inflammation and fibrosis. CONCLUSION: Low-pressure sterile reflux into previously normal kidneys led to mild, focal, chronic interstitial inflammation and fibrosis after 1 year. Imaging findings were normal apart from a subtle decrease in tubular function in two refluxing kidneys.

Original languageEnglish
Pages (from-to)507-515
Number of pages9
JournalRadiology
Volume217
Issue number2
StatePublished - Nov 13 2000
Externally publishedYes

Fingerprint

Vesico-Ureteral Reflux
Swine
Kidney
Pressure
Growth
Technetium Tc 99m Dimercaptosuccinic Acid
Regional Blood Flow
Radionuclide Imaging
Fibrosis
Tomography
Magnetic Resonance Imaging
Inflammation
Ruthenium
Microspheres
Contrast Media
Autopsy
Ultrasonography

Keywords

  • Animals
  • Kidney, CT
  • Kidney, function
  • Kidney, MR
  • Kidney, perfusion
  • Kidney, radionuclide studies
  • Kidney, US
  • Ureter, reflux
  • Voiding cystourethrography

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Paltiel, H. J., Mulkern, R. V., Perez-Atayde, A., Connolly, L. P., Zurakowski, D., Ted Treves, S., & Atala, A. (2000). Effect of chronic, low-pressure, sterile vesicoureteral reflux on renal growth and function in a porcine model: A radiologic and pathologic study. Radiology, 217(2), 507-515.

Effect of chronic, low-pressure, sterile vesicoureteral reflux on renal growth and function in a porcine model : A radiologic and pathologic study. / Paltiel, H. J.; Mulkern, R. V.; Perez-Atayde, A.; Connolly, L. P.; Zurakowski, D.; Ted Treves, S.; Atala, A.

In: Radiology, Vol. 217, No. 2, 13.11.2000, p. 507-515.

Research output: Contribution to journalArticle

Paltiel, HJ, Mulkern, RV, Perez-Atayde, A, Connolly, LP, Zurakowski, D, Ted Treves, S & Atala, A 2000, 'Effect of chronic, low-pressure, sterile vesicoureteral reflux on renal growth and function in a porcine model: A radiologic and pathologic study', Radiology, vol. 217, no. 2, pp. 507-515.
Paltiel HJ, Mulkern RV, Perez-Atayde A, Connolly LP, Zurakowski D, Ted Treves S et al. Effect of chronic, low-pressure, sterile vesicoureteral reflux on renal growth and function in a porcine model: A radiologic and pathologic study. Radiology. 2000 Nov 13;217(2):507-515.
Paltiel, H. J. ; Mulkern, R. V. ; Perez-Atayde, A. ; Connolly, L. P. ; Zurakowski, D. ; Ted Treves, S. ; Atala, A. / Effect of chronic, low-pressure, sterile vesicoureteral reflux on renal growth and function in a porcine model : A radiologic and pathologic study. In: Radiology. 2000 ; Vol. 217, No. 2. pp. 507-515.
@article{d4ac58e6df6d422bb26f390067933a9c,
title = "Effect of chronic, low-pressure, sterile vesicoureteral reflux on renal growth and function in a porcine model: A radiologic and pathologic study",
abstract = "PURPOSE: To study the effects of chronic, low-pressure, sterile vesicoureteral reflux (VUR) on renal growth and function in a porcine model. MATERIALS AND METHODS: Unilateral VUR was created in five pigs, with the contralateral kidney serving as a control. Preoperatively, and 1 year later, ultrasonography, technetium 99m-dimercaptosuccinic acid (DMSA) scintigraphy, contrast material-enhanced computed tomography (CT), and contrast-enhanced magnetic resonance (MR) imaging were performed. Morphologic abnormalities and relative uptake of 99mTc-DMSA were recorded. The postcontrast enhancement ratios for parenchymal regions of interest at CT and MR imaging were determined. Ruthenium 103-labeled microspheres were used to determine regional blood flow. After the pigs were sacrificed, the kidneys were excised, weighed, and analyzed pathologically. RESULTS: Two of five refluxing kidneys had less than 45{\%} function at scintigraphy. One of these two kidneys was small at postmortem examination. There were no other imaging or gross pathologic abnormalities. There was no significant difference in regional blood flow between the refluxing and nonrefluxing kidneys. In all of the operated on kidneys, histologic examination showed focal chronic inflammation and fibrosis. CONCLUSION: Low-pressure sterile reflux into previously normal kidneys led to mild, focal, chronic interstitial inflammation and fibrosis after 1 year. Imaging findings were normal apart from a subtle decrease in tubular function in two refluxing kidneys.",
keywords = "Animals, Kidney, CT, Kidney, function, Kidney, MR, Kidney, perfusion, Kidney, radionuclide studies, Kidney, US, Ureter, reflux, Voiding cystourethrography",
author = "Paltiel, {H. J.} and Mulkern, {R. V.} and A. Perez-Atayde and Connolly, {L. P.} and D. Zurakowski and {Ted Treves}, S. and A. Atala",
year = "2000",
month = "11",
day = "13",
language = "English",
volume = "217",
pages = "507--515",
journal = "Radiology",
issn = "0033-8419",
publisher = "Radiological Society of North America Inc.",
number = "2",

}

TY - JOUR

T1 - Effect of chronic, low-pressure, sterile vesicoureteral reflux on renal growth and function in a porcine model

T2 - A radiologic and pathologic study

AU - Paltiel, H. J.

AU - Mulkern, R. V.

AU - Perez-Atayde, A.

AU - Connolly, L. P.

AU - Zurakowski, D.

AU - Ted Treves, S.

AU - Atala, A.

PY - 2000/11/13

Y1 - 2000/11/13

N2 - PURPOSE: To study the effects of chronic, low-pressure, sterile vesicoureteral reflux (VUR) on renal growth and function in a porcine model. MATERIALS AND METHODS: Unilateral VUR was created in five pigs, with the contralateral kidney serving as a control. Preoperatively, and 1 year later, ultrasonography, technetium 99m-dimercaptosuccinic acid (DMSA) scintigraphy, contrast material-enhanced computed tomography (CT), and contrast-enhanced magnetic resonance (MR) imaging were performed. Morphologic abnormalities and relative uptake of 99mTc-DMSA were recorded. The postcontrast enhancement ratios for parenchymal regions of interest at CT and MR imaging were determined. Ruthenium 103-labeled microspheres were used to determine regional blood flow. After the pigs were sacrificed, the kidneys were excised, weighed, and analyzed pathologically. RESULTS: Two of five refluxing kidneys had less than 45% function at scintigraphy. One of these two kidneys was small at postmortem examination. There were no other imaging or gross pathologic abnormalities. There was no significant difference in regional blood flow between the refluxing and nonrefluxing kidneys. In all of the operated on kidneys, histologic examination showed focal chronic inflammation and fibrosis. CONCLUSION: Low-pressure sterile reflux into previously normal kidneys led to mild, focal, chronic interstitial inflammation and fibrosis after 1 year. Imaging findings were normal apart from a subtle decrease in tubular function in two refluxing kidneys.

AB - PURPOSE: To study the effects of chronic, low-pressure, sterile vesicoureteral reflux (VUR) on renal growth and function in a porcine model. MATERIALS AND METHODS: Unilateral VUR was created in five pigs, with the contralateral kidney serving as a control. Preoperatively, and 1 year later, ultrasonography, technetium 99m-dimercaptosuccinic acid (DMSA) scintigraphy, contrast material-enhanced computed tomography (CT), and contrast-enhanced magnetic resonance (MR) imaging were performed. Morphologic abnormalities and relative uptake of 99mTc-DMSA were recorded. The postcontrast enhancement ratios for parenchymal regions of interest at CT and MR imaging were determined. Ruthenium 103-labeled microspheres were used to determine regional blood flow. After the pigs were sacrificed, the kidneys were excised, weighed, and analyzed pathologically. RESULTS: Two of five refluxing kidneys had less than 45% function at scintigraphy. One of these two kidneys was small at postmortem examination. There were no other imaging or gross pathologic abnormalities. There was no significant difference in regional blood flow between the refluxing and nonrefluxing kidneys. In all of the operated on kidneys, histologic examination showed focal chronic inflammation and fibrosis. CONCLUSION: Low-pressure sterile reflux into previously normal kidneys led to mild, focal, chronic interstitial inflammation and fibrosis after 1 year. Imaging findings were normal apart from a subtle decrease in tubular function in two refluxing kidneys.

KW - Animals

KW - Kidney, CT

KW - Kidney, function

KW - Kidney, MR

KW - Kidney, perfusion

KW - Kidney, radionuclide studies

KW - Kidney, US

KW - Ureter, reflux

KW - Voiding cystourethrography

UR - http://www.scopus.com/inward/record.url?scp=0033735485&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033735485&partnerID=8YFLogxK

M3 - Article

C2 - 11058653

AN - SCOPUS:0033735485

VL - 217

SP - 507

EP - 515

JO - Radiology

JF - Radiology

SN - 0033-8419

IS - 2

ER -