Effect of antibiotic supplementation of Balanced Salt Solution (BSS) infusion during cataract surgery - An experimental model

K. B. Kim, T. K. Chan, G. J. Osterhout, Terrence O'Brien

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose. To study the effect of vancomycin (20 μg/ml) and gentamicin (8 μg/ml) supplemented Balanced Salt Solution (BSS) infusion in reducing bacterial colonization during cataract surgery in an experimental eye model. Methods. An experimental in-vitro eye model using sterilized MARTY Surgical Simulators (Iatrotech, Del Mar, CA) was used in this experiment. In this model, treatment eyes were inoculated with 107 logarithmic growth phase organisms of either Staphylococcus aureus or Pseudomonas aeruginosa into the anterior chamber prior to cataract surgery. Fellow eyes using BSS infusion without antibiotic supplementation during cataract surgery served as controls. Phacoemulsification was performed using the Storz Premiere unit (Storz Instrument Company, St. Louis, MO). The contents of the anterior chamber were aspirated at the end of simulated surgery and inoculated onto appropriate culture media. The culture media were incubated at 37°C for 24 hours and subsequently quantitative bacteriological analysis performed. Results. There was no statistically significant (p > 0.05) difference between eyes which received antibiotic supplemented BSS infusion compared to eyes which received non-antibiotic supplemented BSS infusion in terms of bacterial colonization. Conclusion. This experimental in-vitro model suggests that supplementation of BSS infusion with vancomycin (20 μg/ml) and gentamicin (8 μg/ml) during cataract surgery has no appreciable effect in decreasing colonization of the ocular cavity by Staphylococcus aureus or Pseudomonas aeruginosa.

Original languageEnglish
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

Fingerprint

Cataract
Theoretical Models
Salts
Anti-Bacterial Agents
Anterior Chamber
Vancomycin
Gentamicins
Pseudomonas aeruginosa
Culture Media
Staphylococcus aureus
Mars
Phacoemulsification
Growth

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Effect of antibiotic supplementation of Balanced Salt Solution (BSS) infusion during cataract surgery - An experimental model. / Kim, K. B.; Chan, T. K.; Osterhout, G. J.; O'Brien, Terrence.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

@article{8b0dddae9f4c4d0281d7738c78f6925b,
title = "Effect of antibiotic supplementation of Balanced Salt Solution (BSS) infusion during cataract surgery - An experimental model",
abstract = "Purpose. To study the effect of vancomycin (20 μg/ml) and gentamicin (8 μg/ml) supplemented Balanced Salt Solution (BSS) infusion in reducing bacterial colonization during cataract surgery in an experimental eye model. Methods. An experimental in-vitro eye model using sterilized MARTY Surgical Simulators (Iatrotech, Del Mar, CA) was used in this experiment. In this model, treatment eyes were inoculated with 107 logarithmic growth phase organisms of either Staphylococcus aureus or Pseudomonas aeruginosa into the anterior chamber prior to cataract surgery. Fellow eyes using BSS infusion without antibiotic supplementation during cataract surgery served as controls. Phacoemulsification was performed using the Storz Premiere unit (Storz Instrument Company, St. Louis, MO). The contents of the anterior chamber were aspirated at the end of simulated surgery and inoculated onto appropriate culture media. The culture media were incubated at 37°C for 24 hours and subsequently quantitative bacteriological analysis performed. Results. There was no statistically significant (p > 0.05) difference between eyes which received antibiotic supplemented BSS infusion compared to eyes which received non-antibiotic supplemented BSS infusion in terms of bacterial colonization. Conclusion. This experimental in-vitro model suggests that supplementation of BSS infusion with vancomycin (20 μg/ml) and gentamicin (8 μg/ml) during cataract surgery has no appreciable effect in decreasing colonization of the ocular cavity by Staphylococcus aureus or Pseudomonas aeruginosa.",
author = "Kim, {K. B.} and Chan, {T. K.} and Osterhout, {G. J.} and Terrence O'Brien",
year = "1996",
month = "2",
day = "15",
language = "English",
volume = "37",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "3",

}

TY - JOUR

T1 - Effect of antibiotic supplementation of Balanced Salt Solution (BSS) infusion during cataract surgery - An experimental model

AU - Kim, K. B.

AU - Chan, T. K.

AU - Osterhout, G. J.

AU - O'Brien, Terrence

PY - 1996/2/15

Y1 - 1996/2/15

N2 - Purpose. To study the effect of vancomycin (20 μg/ml) and gentamicin (8 μg/ml) supplemented Balanced Salt Solution (BSS) infusion in reducing bacterial colonization during cataract surgery in an experimental eye model. Methods. An experimental in-vitro eye model using sterilized MARTY Surgical Simulators (Iatrotech, Del Mar, CA) was used in this experiment. In this model, treatment eyes were inoculated with 107 logarithmic growth phase organisms of either Staphylococcus aureus or Pseudomonas aeruginosa into the anterior chamber prior to cataract surgery. Fellow eyes using BSS infusion without antibiotic supplementation during cataract surgery served as controls. Phacoemulsification was performed using the Storz Premiere unit (Storz Instrument Company, St. Louis, MO). The contents of the anterior chamber were aspirated at the end of simulated surgery and inoculated onto appropriate culture media. The culture media were incubated at 37°C for 24 hours and subsequently quantitative bacteriological analysis performed. Results. There was no statistically significant (p > 0.05) difference between eyes which received antibiotic supplemented BSS infusion compared to eyes which received non-antibiotic supplemented BSS infusion in terms of bacterial colonization. Conclusion. This experimental in-vitro model suggests that supplementation of BSS infusion with vancomycin (20 μg/ml) and gentamicin (8 μg/ml) during cataract surgery has no appreciable effect in decreasing colonization of the ocular cavity by Staphylococcus aureus or Pseudomonas aeruginosa.

AB - Purpose. To study the effect of vancomycin (20 μg/ml) and gentamicin (8 μg/ml) supplemented Balanced Salt Solution (BSS) infusion in reducing bacterial colonization during cataract surgery in an experimental eye model. Methods. An experimental in-vitro eye model using sterilized MARTY Surgical Simulators (Iatrotech, Del Mar, CA) was used in this experiment. In this model, treatment eyes were inoculated with 107 logarithmic growth phase organisms of either Staphylococcus aureus or Pseudomonas aeruginosa into the anterior chamber prior to cataract surgery. Fellow eyes using BSS infusion without antibiotic supplementation during cataract surgery served as controls. Phacoemulsification was performed using the Storz Premiere unit (Storz Instrument Company, St. Louis, MO). The contents of the anterior chamber were aspirated at the end of simulated surgery and inoculated onto appropriate culture media. The culture media were incubated at 37°C for 24 hours and subsequently quantitative bacteriological analysis performed. Results. There was no statistically significant (p > 0.05) difference between eyes which received antibiotic supplemented BSS infusion compared to eyes which received non-antibiotic supplemented BSS infusion in terms of bacterial colonization. Conclusion. This experimental in-vitro model suggests that supplementation of BSS infusion with vancomycin (20 μg/ml) and gentamicin (8 μg/ml) during cataract surgery has no appreciable effect in decreasing colonization of the ocular cavity by Staphylococcus aureus or Pseudomonas aeruginosa.

UR - http://www.scopus.com/inward/record.url?scp=0006403174&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0006403174&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0006403174

VL - 37

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 3

ER -