Purpose. To study the effect of vancomycin (20 μg/ml) and gentamicin (8 μg/ml) supplemented Balanced Salt Solution (BSS) infusion in reducing bacterial colonization during cataract surgery in an experimental eye model. Methods. An experimental in-vitro eye model using sterilized MARTY Surgical Simulators (Iatrotech, Del Mar, CA) was used in this experiment. In this model, treatment eyes were inoculated with 107 logarithmic growth phase organisms of either Staphylococcus aureus or Pseudomonas aeruginosa into the anterior chamber prior to cataract surgery. Fellow eyes using BSS infusion without antibiotic supplementation during cataract surgery served as controls. Phacoemulsification was performed using the Storz Premiere unit (Storz Instrument Company, St. Louis, MO). The contents of the anterior chamber were aspirated at the end of simulated surgery and inoculated onto appropriate culture media. The culture media were incubated at 37°C for 24 hours and subsequently quantitative bacteriological analysis performed. Results. There was no statistically significant (p > 0.05) difference between eyes which received antibiotic supplemented BSS infusion compared to eyes which received non-antibiotic supplemented BSS infusion in terms of bacterial colonization. Conclusion. This experimental in-vitro model suggests that supplementation of BSS infusion with vancomycin (20 μg/ml) and gentamicin (8 μg/ml) during cataract surgery has no appreciable effect in decreasing colonization of the ocular cavity by Staphylococcus aureus or Pseudomonas aeruginosa.
|Original language||English (US)|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - Feb 15 1996|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience