Abstract
We have carried out studies on the effect of ammonium chloride on the astrocyte benzodiazepine receptor. Scatchard analysis of the binding of [3H]Ro-5-4864 to homogenates prepared from primary astrocyte cultures showed a significant decrease in Kd (27% with 2 mM NH4Cl; 32% with 5 mM NH4Cl; 25% with 10 mM NH4Cl) and Bmax (14% with 10 mM NH4Cl). These findings indicate that ammonium chloride can affect the astrocyte benzodiazepine receptor, and that such receptor changes may contribute to ammonia-induced encephalopathy.
| Original language | English |
|---|---|
| Pages (from-to) | 362-365 |
| Number of pages | 4 |
| Journal | Brain Research |
| Volume | 493 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 31 1989 |
Fingerprint
Keywords
- Ammonia
- Astrocyte
- Benzodiazepine receptor
- Hepatic encephalopathy
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
Effect of ammonium chloride on the astrocyte benzodiazepine receptor. / Ducis, Ilze; Norenberg, Luz Oliva B; Norenberg, Michael D.
In: Brain Research, Vol. 493, No. 2, 31.07.1989, p. 362-365.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of ammonium chloride on the astrocyte benzodiazepine receptor
AU - Ducis, Ilze
AU - Norenberg, Luz Oliva B
AU - Norenberg, Michael D
PY - 1989/7/31
Y1 - 1989/7/31
N2 - We have carried out studies on the effect of ammonium chloride on the astrocyte benzodiazepine receptor. Scatchard analysis of the binding of [3H]Ro-5-4864 to homogenates prepared from primary astrocyte cultures showed a significant decrease in Kd (27% with 2 mM NH4Cl; 32% with 5 mM NH4Cl; 25% with 10 mM NH4Cl) and Bmax (14% with 10 mM NH4Cl). These findings indicate that ammonium chloride can affect the astrocyte benzodiazepine receptor, and that such receptor changes may contribute to ammonia-induced encephalopathy.
AB - We have carried out studies on the effect of ammonium chloride on the astrocyte benzodiazepine receptor. Scatchard analysis of the binding of [3H]Ro-5-4864 to homogenates prepared from primary astrocyte cultures showed a significant decrease in Kd (27% with 2 mM NH4Cl; 32% with 5 mM NH4Cl; 25% with 10 mM NH4Cl) and Bmax (14% with 10 mM NH4Cl). These findings indicate that ammonium chloride can affect the astrocyte benzodiazepine receptor, and that such receptor changes may contribute to ammonia-induced encephalopathy.
KW - Ammonia
KW - Astrocyte
KW - Benzodiazepine receptor
KW - Hepatic encephalopathy
UR - http://www.scopus.com/inward/record.url?scp=0024324665&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024324665&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(89)91171-2
DO - 10.1016/0006-8993(89)91171-2
M3 - Article
C2 - 2548671
AN - SCOPUS:0024324665
VL - 493
SP - 362
EP - 365
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 2
ER -