Effect of ammonium chloride on the astrocyte benzodiazepine receptor

Ilze Ducis, Luz Oliva B Norenberg, Michael D Norenberg

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We have carried out studies on the effect of ammonium chloride on the astrocyte benzodiazepine receptor. Scatchard analysis of the binding of [3H]Ro-5-4864 to homogenates prepared from primary astrocyte cultures showed a significant decrease in Kd (27% with 2 mM NH4Cl; 32% with 5 mM NH4Cl; 25% with 10 mM NH4Cl) and Bmax (14% with 10 mM NH4Cl). These findings indicate that ammonium chloride can affect the astrocyte benzodiazepine receptor, and that such receptor changes may contribute to ammonia-induced encephalopathy.

Original languageEnglish
Pages (from-to)362-365
Number of pages4
JournalBrain Research
Volume493
Issue number2
DOIs
StatePublished - Jul 31 1989

Fingerprint

Ammonium Chloride
GABA-A Receptors
Astrocytes
Brain Diseases
Ammonia

Keywords

  • Ammonia
  • Astrocyte
  • Benzodiazepine receptor
  • Hepatic encephalopathy

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Effect of ammonium chloride on the astrocyte benzodiazepine receptor. / Ducis, Ilze; Norenberg, Luz Oliva B; Norenberg, Michael D.

In: Brain Research, Vol. 493, No. 2, 31.07.1989, p. 362-365.

Research output: Contribution to journalArticle

Ducis, Ilze ; Norenberg, Luz Oliva B ; Norenberg, Michael D. / Effect of ammonium chloride on the astrocyte benzodiazepine receptor. In: Brain Research. 1989 ; Vol. 493, No. 2. pp. 362-365.
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