Effect of ammonium chloride on the astrocyte benzodiazepine receptor

Ilze Ducis; Luz Oliva B. Norenberg; Michael D. Norenberg

doi:10.1016/0006-8993(89)91171-2

Effect of ammonium chloride on the astrocyte benzodiazepine receptor

Ilze Ducis, Luz Oliva B. Norenberg, Michael D. Norenberg

Pathology

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

We have carried out studies on the effect of ammonium chloride on the astrocyte benzodiazepine receptor. Scatchard analysis of the binding of [³H]Ro-5-4864 to homogenates prepared from primary astrocyte cultures showed a significant decrease in K_d (27% with 2 mM NH₄Cl; 32% with 5 mM NH₄Cl; 25% with 10 mM NH₄Cl) and B_max (14% with 10 mM NH₄Cl). These findings indicate that ammonium chloride can affect the astrocyte benzodiazepine receptor, and that such receptor changes may contribute to ammonia-induced encephalopathy.

Original language	English (US)
Pages (from-to)	362-365
Number of pages	4
Journal	Brain research
Volume	493
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(89)91171-2
State	Published - Jul 31 1989

Keywords

Ammonia
Astrocyte
Benzodiazepine receptor
Hepatic encephalopathy

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
Neuroscience(all)

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10.1016/0006-8993(89)91171-2

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title = "Effect of ammonium chloride on the astrocyte benzodiazepine receptor",

abstract = "We have carried out studies on the effect of ammonium chloride on the astrocyte benzodiazepine receptor. Scatchard analysis of the binding of [3H]Ro-5-4864 to homogenates prepared from primary astrocyte cultures showed a significant decrease in Kd (27% with 2 mM NH4Cl; 32% with 5 mM NH4Cl; 25% with 10 mM NH4Cl) and Bmax (14% with 10 mM NH4Cl). These findings indicate that ammonium chloride can affect the astrocyte benzodiazepine receptor, and that such receptor changes may contribute to ammonia-induced encephalopathy.",

keywords = "Ammonia, Astrocyte, Benzodiazepine receptor, Hepatic encephalopathy",

author = "Ilze Ducis and Norenberg, {Luz Oliva B.} and Norenberg, {Michael D.}",

note = "Funding Information: This work has been supported by NIH Grant RO1 DK38153-02 and by the Veterans Administration. We thank Dr. Joseph T. Neary for the thoughtful reading of the manuscript and Celeste Bowen for its preparation. We also thank Edward Forster and Carmen Sabria for their technical assistance.",

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T1 - Effect of ammonium chloride on the astrocyte benzodiazepine receptor

AU - Ducis, Ilze

AU - Norenberg, Luz Oliva B.

AU - Norenberg, Michael D.

N1 - Funding Information: This work has been supported by NIH Grant RO1 DK38153-02 and by the Veterans Administration. We thank Dr. Joseph T. Neary for the thoughtful reading of the manuscript and Celeste Bowen for its preparation. We also thank Edward Forster and Carmen Sabria for their technical assistance.

PY - 1989/7/31

Y1 - 1989/7/31

N2 - We have carried out studies on the effect of ammonium chloride on the astrocyte benzodiazepine receptor. Scatchard analysis of the binding of [3H]Ro-5-4864 to homogenates prepared from primary astrocyte cultures showed a significant decrease in Kd (27% with 2 mM NH4Cl; 32% with 5 mM NH4Cl; 25% with 10 mM NH4Cl) and Bmax (14% with 10 mM NH4Cl). These findings indicate that ammonium chloride can affect the astrocyte benzodiazepine receptor, and that such receptor changes may contribute to ammonia-induced encephalopathy.

AB - We have carried out studies on the effect of ammonium chloride on the astrocyte benzodiazepine receptor. Scatchard analysis of the binding of [3H]Ro-5-4864 to homogenates prepared from primary astrocyte cultures showed a significant decrease in Kd (27% with 2 mM NH4Cl; 32% with 5 mM NH4Cl; 25% with 10 mM NH4Cl) and Bmax (14% with 10 mM NH4Cl). These findings indicate that ammonium chloride can affect the astrocyte benzodiazepine receptor, and that such receptor changes may contribute to ammonia-induced encephalopathy.

KW - Ammonia

KW - Astrocyte

KW - Benzodiazepine receptor

KW - Hepatic encephalopathy

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Effect of ammonium chloride on the astrocyte benzodiazepine receptor

Abstract

Keywords

ASJC Scopus subject areas

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